Immunohistochemical Features of MMP-9 and PSTAT1 in Granuloma Annulare and Sarcoidosis: A Comparative Study of 62 Cases

Overview

Journal J Immunol Res

Publisher Wiley

Specialty Allergy & Immunology

Date 2023 Mar 24

PMID 36959923

Authors

Ze Wu

Linghui Li

Hui Qu

Rui-Qun Qi

Jun Niu

Affiliations

Soon will be listed here.

Abstract

Background: Granuloma annulare (GA) and sarcoidosis are granulomatous inflammatory diseases that share similarities.

Objective: To identify the histological and immunohistochemical (IHC) features of GA and sarcoidosis.

Methods: A retrospective review of 36 patients with GA and 26 with sarcoidosis was performed. Results from hematoxylin and eosin (H&E) staining and IHC staining of MMP-9 and pSTAT1 within the skin lesions of GA and sarcoidosis were analyzed, and random forest was applied for developing a predictive model.

Results: Significantly greater expressions of MMP-9 (especially in elastic fibers, EFs, < 0.0001) and pSTAT1 ( = 0.0003) were observed in lesion samples of GA versus sarcoidosis patients. In GA patients, MMP-9 was significantly upregulated in the interstitial type ( = 0.0222), while staining of pSTAT1 was positively correlated with the area of mucinous collagen in palisading GA ( = 0.5356, = 0.0484). In sarcoidosis patients, MMP-9 ( = -0.7127, = 0.0009) and pSTAT1 ( = -0.5604, = 0.0067) were found to show stronger expressions in lesions with less lymphocyte infiltration. The predictive model demonstrated an AUC of 0.9675.

Conclusion: These results indicate that MMP-9 and pSTAT1 might exert roles in granulomatous inflammation in different modes, and the presence of more robust MMP-9 staining in EFs appears to be more suggestive of GA.

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