Investigation of Taurine and Aqueous Garlic Extract Diet Supplementation Effect on the Healing of Rat Osteoporotic Fractures

Overview

Journal Turk J Med Sci

Specialty General Medicine

Date 2023 Mar 22

PMID 36945935

Authors

Mesut Misirlioglu

Izzet Bingol

Coskun Genc

Aylin Akbulut

Mert Ocak

Neziha Yagmur Diker

Ahmet Ozmeric

Gokhan Koca

Melike Bahcecitapar

Mehmet Senes

Aysegul Firat

Fevziye Figen Kaymaz

Hakan Hamdi Celik

Iffet Irem Cankaya

Meliha Korkmaz

Affiliations

Soon will be listed here.

Abstract

Background: We have evaluated the effects of taurine and aqueous garlic extract (AGE) as a dietary supplement on osteoporotic fracture (OPF) healing in the ovariectomized rat femur fracture model.

Methods: In this experimental animal study,twenty-four osteoporosis-remodeled female Wistar albino rats were randomly divided into 3 groups (n: 8) according to their supplemented diet; control, taurine, and AGE groups. Unilateral femur middiaphysis mini-open osteotomy was stabilized with Kirschner wires. Six weeks after osteotomy, the rats were sacrificed before the femurs were harvested and OPF healing was evaluated with biochemical, histologic, microcomputed-tomography, and scintigraphic methods.

Results: As an indicator of the antiosteoporotic effect, the calcium levels of the taurine group were significantly lower than the AGE and control groups in biochemical analyzes (p < 0.01). In histological studies, the new bone diameter and new bone volume values of the taurine group were significantly higher than the control group (p = 0.002 and p = 0.032, respectively), while higher trabecular-compact callus was observed in the taurine and AGE groups, respectively, compared to the control group. In morphological analyses, taurine and AGE groups had significantly higher bone volume/tissue volume, trabecular number, bone surface density, and lower trabecular separation than the control group (p < 0.05). The scintigraphic imaging showed a significant increase in osteoblastic activity of the taurine group compared to the control group (p = 0.005).

Discussion: Taurine and AGE have positive anabolic effects, respectively, on the healing of OPFs, demonstrated by biochemical, histological, morphological, and scintigraphic methods.

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