The Root Meristem Growth Factor Positively Regulates Chinese Cabbage to Infection of Clubroot Disease Caused by

Overview

Journal Hortic Res

Publisher Oxford University Press

Date 2023 Mar 20

PMID 36938571

Authors

Wenjie Ge

Jing Zhang

Hui Feng

Yilian Wang

Ruiqin Ji

Affiliations

Soon will be listed here.

Abstract

Chinese cabbage has a high annual demand in China. However, clubroot disease caused by the infection of seriously affects its yield. Transcriptome analysis identified a root meristem growth factor 6 () as significantly up-regulated in Chinese cabbage roots infected with . Quantitative reverse-transcription polymerase chain reaction and hybridization analysis showed higher expression in susceptible materials than in resistant materials. After infection, expression was significantly up-regulated, especially in susceptible materials. Gene function analysis showed that the roots of mutant grew faster than the wild-type, and delayed the infection progress of . A Protein, nuclear transcription factor Y subunit C (BrNF-YC), was screened from yeast two-hybrid library of Chinese cabbage induced by , and verified to interact with BrRGF6 by yeast two-hybrid co-transfer. Yeast one-hybrid and β-Glucuronidase activity analysis showed that BrNF-YC could directly bind to and strongly activate the promoter of . Transgenic verification showed that or silenced Chinese cabbage significantly decreased the expression of , accelerated root development, and reduced incidence of clubroot disease. However, after overexpression of or , the phenotype showed a reverse trend. Therefore, silencing accelerated root growth and enhanced resistance to clubroot disease, which was regulated by BrNF-YC.

References

Chu M, Song T, Falk K, Zhang X, Liu X, Chang A . Fine mapping of Rcr1 and analyses of its effect on transcriptome patterns during infection by Plasmodiophora brassicae. BMC Genomics. 2014; 15:1166. PMC: 4326500. DOI: 10.1186/1471-2164-15-1166. View

Ma X, Yu T, Li X, Cao X, Ma J, Chen J . Overexpression of GmNFYA5 confers drought tolerance to transgenic Arabidopsis and soybean plants. BMC Plant Biol. 2020; 20(1):123. PMC: 7082914. DOI: 10.1186/s12870-020-02337-z. View

Waadt R, Schmidt L, Lohse M, Hashimoto K, Bock R, Kudla J . Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta. Plant J. 2008; 56(3):505-16. DOI: 10.1111/j.1365-313X.2008.03612.x. View

Hackenberg D, Keetman U, Grimm B . Homologous NF-YC2 subunit from Arabidopsis and tobacco is activated by photooxidative stress and induces flowering. Int J Mol Sci. 2012; 13(3):3458-3477. PMC: 3317722. DOI: 10.3390/ijms13033458. View

Meschini E, Blanco F, Zanetti M, Beker M, Kuster H, Puhler A . Host genes involved in nodulation preference in common bean (Phaseolus vulgaris)-rhizobium etli symbiosis revealed by suppressive subtractive hybridization. Mol Plant Microbe Interact. 2008; 21(4):459-68. DOI: 10.1094/MPMI-21-4-0459. View

Lv M, Liu Y, Wu Y, Zhang J, Liu X, Ji R . An Improved Technique for Isolation and Characterization of Single-Spore Isolates of . Plant Dis. 2021; 105(12):3932-3938. DOI: 10.1094/PDIS-03-21-0480-RE. View

Dolfini D, Zambelli F, Pavesi G, Mantovani R . A perspective of promoter architecture from the CCAAT box. Cell Cycle. 2009; 8(24):4127-37. DOI: 10.4161/cc.8.24.10240. View

Verma V, Ravindran P, Kumar P . Plant hormone-mediated regulation of stress responses. BMC Plant Biol. 2016; 16:86. PMC: 4831116. DOI: 10.1186/s12870-016-0771-y. View

Meng L, Buchanan B, Feldman L, Luan S . CLE-like (CLEL) peptides control the pattern of root growth and lateral root development in Arabidopsis. Proc Natl Acad Sci U S A. 2012; 109(5):1760-5. PMC: 3277184. DOI: 10.1073/pnas.1119864109. View

10.

Fernandez A, Drozdzecki A, Hoogewijs K, Nguyen A, Beeckman T, Madder A . Transcriptional and functional classification of the GOLVEN/ROOT GROWTH FACTOR/CLE-like signaling peptides reveals their role in lateral root and hair formation. Plant Physiol. 2013; 161(2):954-70. PMC: 3561032. DOI: 10.1104/pp.112.206029. View

11.

Yang Y, Ahammed G, Wu C, Fan S, Zhou Y . Crosstalk among Jasmonate, Salicylate and Ethylene Signaling Pathways in Plant Disease and Immune Responses. Curr Protein Pept Sci. 2015; 16(5):450-61. DOI: 10.2174/1389203716666150330141638. View

12.

Matsuzaki Y, Ogawa-Ohnishi M, Mori A, Matsubayashi Y . Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis. Science. 2010; 329(5995):1065-7. DOI: 10.1126/science.1191132. View

13.

Yuan Y, Qin L, Su H, Yang S, Wei X, Wang Z . Transcriptome and Coexpression Network Analyses Reveal Hub Genes in Chinese Cabbage ( L. ssp. ) During Different Stages of Infection. Front Plant Sci. 2021; 12:650252. PMC: 8383047. DOI: 10.3389/fpls.2021.650252. View

14.

Ripodas C, Castaingts M, Clua J, Villafane J, Blanco F, Zanetti M . The PvNF-YA1 and PvNF-YB7 Subunits of the Heterotrimeric NF-Y Transcription Factor Influence Strain Preference in the Symbiosis. Front Plant Sci. 2019; 10:221. PMC: 6403126. DOI: 10.3389/fpls.2019.00221. View

15.

Mitsuda N, Ikeda M, Takada S, Takiguchi Y, Kondou Y, Yoshizumi T . Efficient yeast one-/two-hybrid screening using a library composed only of transcription factors in Arabidopsis thaliana. Plant Cell Physiol. 2010; 51(12):2145-51. DOI: 10.1093/pcp/pcq161. View

16.

Pflieger S, Blanchet S, Camborde L, Drugeon G, Rousseau A, Noizet M . Efficient virus-induced gene silencing in Arabidopsis using a 'one-step' TYMV-derived vector. Plant J. 2008; 56(4):678-90. DOI: 10.1111/j.1365-313X.2008.03620.x. View

17.

Laloum T, Baudin M, Frances L, Lepage A, Billault-Penneteau B, Cerri M . Two CCAAT-box-binding transcription factors redundantly regulate early steps of the legume-rhizobia endosymbiosis. Plant J. 2014; 79(5):757-68. DOI: 10.1111/tpj.12587. View

18.

Su H, Cao Y, Ku L, Yao W, Cao Y, Ren Z . Dual functions of ZmNF-YA3 in photoperiod-dependent flowering and abiotic stress responses in maize. J Exp Bot. 2018; 69(21):5177-5189. DOI: 10.1093/jxb/ery299. View

19.

Galindo-Gonzalez L, Manolii V, Hwang S, Strelkov S . Response of to Involves Salicylic Acid-Mediated Immunity: An RNA-Seq-Based Study. Front Plant Sci. 2020; 11:1025. PMC: 7367028. DOI: 10.3389/fpls.2020.01025. View

20.

Ghorbani S, Hoogewijs K, Pecenkova T, Fernandez A, Inze A, Eeckhout D . The SBT6.1 subtilase processes the GOLVEN1 peptide controlling cell elongation. J Exp Bot. 2016; 67(16):4877-87. PMC: 4983112. DOI: 10.1093/jxb/erw241. View