Antimicrobial Effect Against of Advanced and Injectable Platelet-rich Fibrin from Patients with Periodontal Diseases Versus Periodontally Healthy Subjects

Overview

Journal J Oral Biol Craniofac Res

Publisher Elsevier

Date 2023 Mar 20

PMID 36937558

Authors

Thuy Anh Vu Pham

Thao Thi Phuong Tran

Affiliations

Soon will be listed here.

Abstract

Objectives: Advanced platelet-rich fibrin (A-PRF+) and injectable platelet-rich fibrin (i-PRF) have recently been developed and used in periodontal therapy. Few studies have contrasted the antibacterial effectiveness of these autologous materials derived from individuals with healthy gums, gingivitis, and periodontitis. This study aimed to compare the antimicrobial effects of these PRF materials against the periodontal pathogenic bacterium () in patients with different periodontal conditions.

Methods: Blood samples were collected from periodontally healthy individuals, patients with gingivitis, or patients with periodontitis to prepare A-PRF+ and i-PRF. The antibacterial capacity of these materials was evaluated through antibiofilm formation, biofilm susceptibility, and the time-kill assay over a 48-h period.

Results: A-PRF+ and i-PRF from each patient groups interfered with 's ability to form biofilm on the test tube surface, and the effect of i-PRF was significantly different among the patient groups. In contrast, these plasma preparation had a weak impact on mature biofilm. For products from the gingivitis and periodontitis groups, these effects were significantly stronger for i-PRF than A-PRF+ (p = 0.012 and p = 0.004, respectively). All plasma preparations inhibited growth in the first 12 h after application, and i-PRF exhibited a significantly greater antimicrobial effect than A-PRF + at each time point.

Conclusion: A-PRF+ and i-PRF in all three patient groups could inhibit the growth of Aa , and i-PRF from patients with periodontitis exhibited a more significant effect than PRF from the other groups.

Citing Articles

Platelet-rich fibrin obtained from different protocols affects the formation of the multispecies subgingival biofilm associated with periodontitis.

Uyeda F, Quilles Vargas G, Matias Malavazi L, Macedo T, Gomes A, Bueno M J Oral Microbiol. 2025; 17(1):2445598.

PMID: 39801747 PMC: 11722119. DOI: 10.1080/20002297.2024.2445598.

L-PRF in extra-oral wound care.

Pinto N, Yu J, Koirala S, Mourao C, Andrade C, Rescigno E Periodontol 2000. 2024; 97(1):342-362.

PMID: 39305000 PMC: 11808448. DOI: 10.1111/prd.12605.

A yeast-malic acid crosslinker/polyacrylic acid hydrogel containing doxycycline for the treatment of periodontitis.

Qaiser M, Asmatullah M, Shahwar D, Aqeel M, Ameer N, Mahmood K RSC Adv. 2024; 14(35):25174-25189.

PMID: 39139228 PMC: 11318522. DOI: 10.1039/d4ra02638a.

Socket Preservation Using Platelet-Rich Fibrin and Free Gingival Grafts.

Afifi H, Nasr S, BinShabaib M, Alharthi S, Shoeib M Int Dent J. 2024; 75(1):345-351.

PMID: 38987050 PMC: 11806327. DOI: 10.1016/j.identj.2024.06.005.

How to explain the beneficial effects of leukocyte- and platelet-rich fibrin.

Blanco J, Garcia A, Garcia Alonso A, Hermida-Nogueira L, Castro A Periodontol 2000. 2024; 97(1):74-94.

PMID: 38923566 PMC: 11808445. DOI: 10.1111/prd.12570.

References

Ghanaati S, Booms P, Orlowska A, Kubesch A, Lorenz J, Rutkowski J . Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol. 2014; 40(6):679-89. DOI: 10.1563/aaid-joi-D-14-00138. View

Monteiro A, Jardini M, Alves S, Giampaoli V, Aubin E, Figueiredo Neto A . Cardiovascular disease parameters in periodontitis. J Periodontol. 2009; 80(3):378-88. DOI: 10.1902/jop.2009.080431. View

DAiuto F, Parkar M, Andreou G, Suvan J, Brett P, Ready D . Periodontitis and systemic inflammation: control of the local infection is associated with a reduction in serum inflammatory markers. J Dent Res. 2004; 83(2):156-60. DOI: 10.1177/154405910408300214. View

Rodriguez Sanchez F, Verspecht T, Castro A, Pauwels M, Andres C, Quirynen M . Antimicrobial Mechanisms of Leucocyte- and Platelet Rich Fibrin Exudate Against Planktonic and Within Multi-Species Biofilm: A Pilot Study. Front Cell Infect Microbiol. 2021; 11:722499. PMC: 8548765. DOI: 10.3389/fcimb.2021.722499. View

Papapanou P, Sanz M, Buduneli N, Dietrich T, Feres M, Fine D . Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol. 2018; 89 Suppl 1:S173-S182. DOI: 10.1002/JPER.17-0721. View

Karde P, Sethi K, Mahale S, Khedkar S, Patil A, Joshi C . Comparative evaluation of platelet count and antimicrobial efficacy of injectable platelet-rich fibrin with other platelet concentrates: An study. J Indian Soc Periodontol. 2018; 21(2):97-101. PMC: 5771122. DOI: 10.4103/jisp.jisp_201_17. View

Fine D, Goncharoff P, Schreiner H, Chang K, Furgang D, Figurski D . Colonization and persistence of rough and smooth colony variants of Actinobacillus actinomycetemcomitans in the mouths of rats. Arch Oral Biol. 2001; 46(11):1065-78. DOI: 10.1016/s0003-9969(01)00067-x. View

Jasmine S, A T, Janarthanan K, Krishnamoorthy R, Alshatwi A . Antimicrobial and antibiofilm potential of injectable platelet rich fibrin-a second-generation platelet concentrate-against biofilm producing oral staphylococcus isolates. Saudi J Biol Sci. 2020; 27(1):41-46. PMC: 6933211. DOI: 10.1016/j.sjbs.2019.04.012. View

Anitua E, Alonso R, Girbau C, Aguirre J, Muruzabal F, Orive G . Antibacterial effect of plasma rich in growth factors (PRGF®-Endoret®) against Staphylococcus aureus and Staphylococcus epidermidis strains. Clin Exp Dermatol. 2012; 37(6):652-7. DOI: 10.1111/j.1365-2230.2011.04303.x. View

10.

Yin W, Xu S, Wang Y, Zhang Y, Chou S, Galperin M . Ways to control harmful biofilms: prevention, inhibition, and eradication. Crit Rev Microbiol. 2020; 47(1):57-78. PMC: 7954276. DOI: 10.1080/1040841X.2020.1842325. View

11.

Egle K, Salma I, Dubnika A . From Blood to Regenerative Tissue: How Autologous Platelet-Rich Fibrin Can Be Combined with Other Materials to Ensure Controlled Drug and Growth Factor Release. Int J Mol Sci. 2021; 22(21). PMC: 8583771. DOI: 10.3390/ijms222111553. View

12.

Shah R, M G T, Thomas R, Mehta D . An Update on the Protocols and Biologic Actions of Platelet Rich Fibrin in Dentistry. Eur J Prosthodont Restor Dent. 2017; 25(2):64-72. DOI: 10.1922/EJPRD_01690Shah09. View

13.

Buhlin K, Gustafsson A, Pockley A, Frostegard J, Klinge B . Risk factors for cardiovascular disease in patients with periodontitis. Eur Heart J. 2003; 24(23):2099-107. DOI: 10.1016/j.ehj.2003.09.016. View

14.

Fujioka-Kobayashi M, Miron R, Hernandez M, Kandalam U, Zhang Y, Choukroun J . Optimized Platelet-Rich Fibrin With the Low-Speed Concept: Growth Factor Release, Biocompatibility, and Cellular Response. J Periodontol. 2016; 88(1):112-121. DOI: 10.1902/jop.2016.160443. View

15.

Lasserre J, Brecx M, Toma S . Oral Microbes, Biofilms and Their Role in Periodontal and Peri-Implant Diseases. Materials (Basel). 2018; 11(10). PMC: 6213094. DOI: 10.3390/ma11101802. View

16.

Kour P, Pudakalkatti P, Vas A, Das S, Padmanabhan S . Comparative Evaluation of Antimicrobial Efficacy of Platelet-rich Plasma, Platelet-rich Fibrin, and Injectable Platelet-rich Fibrin on the Standard Strains of and . Contemp Clin Dent. 2018; 9(Suppl 2):S325-S330. PMC: 6169270. DOI: 10.4103/ccd.ccd_367_18. View

17.

Paharik A, Horswill A . The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response. Microbiol Spectr. 2016; 4(2). PMC: 4887152. DOI: 10.1128/microbiolspec.VMBF-0022-2015. View

18.

Rozalski M, Micota B, Sadowska B, Paszkiewicz M, Wieckowska-Szakiel M, Rozalska B . Antimicrobial/anti-biofilm activity of expired blood platelets and their released products. Postepy Hig Med Dosw (Online). 2013; 67:321-5. DOI: 10.5604/17322693.1046009. View

19.

Henderson B, Nair S, Ward J, Wilson M . Molecular pathogenicity of the oral opportunistic pathogen Actinobacillus actinomycetemcomitans. Annu Rev Microbiol. 2003; 57:29-55. DOI: 10.1146/annurev.micro.57.030502.090908. View

20.

Yang L, Hu S, Yan M, Yang J, Tsou S, Lin Y . Antimicrobial activity of platelet-rich plasma and other plasma preparations against periodontal pathogens. J Periodontol. 2014; 86(2):310-8. DOI: 10.1902/jop.2014.140373. View