Employing Non-canonical Amino Acids Towards the Immobilization of a Hyperthermophilic Enzyme to Increase Protein Stability

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2023 Mar 17

PMID 36926306

Authors

Hannah J Switzer

Christina A Howard

John F Halonski

Emily M Peairs

Nolan Smith

Maddy P Zamecnik

Sanjana Verma

Douglas D Young

Affiliations

Soon will be listed here.

Abstract

A carboxylesterase derived from P1 was immobilized onto an epoxy-activated Sepharose resin non-canonical amino acids. The immobilized enzyme exhibited heightened performance in organic solvents, recyclability, and stability at room temperature for over two years. The incorporation of a non-canonical amino acid afforded a high degree of control over the bioorthogonal immobilization reaction. These results indicate that the specificity conferred by genetic code expansion produces advantages in protein immobilization and broadens the utility of such proteins to non-biological settings.

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