An Evaluation of the Thermoregulatory Potential of in Ovo Delivered Bioactive Substances (probiotic, Folic Acid, and Essential Oil) in Broiler Chickens

Overview

Journal Poult Sci

Publisher Elsevier

Specialty Veterinary Medicine

Date 2023 Mar 16

PMID 36924590

Authors

Samson Oladokun

Sami Dridi

Deborah Adewole

Affiliations

Soon will be listed here.

Abstract

Mitigating the negative effects of heat stress (HS) is a critical challenge for the global poultry industry. This study evaluated the thermoregulatory potential of 3 in ovo delivered bioactive substances using selected gut health parameters. Eggs were incubated and allotted to 5 groups, and respective bioactive substances delivered. These groups included-the noninjected, in ovo saline, in ovo folic acid (FA), in ovo probiotics (P), and in ovo essential oil (EO). At hatch, chicks were assigned to 5 new posthatch treatment combinations, including A) Negative control (NC)-noninjected eggs offered a basal corn-wheat-soybean diet, B) Antibiotics-NC + 0.05% bacitracin methylene disalicylate, C) In ovo FA-eggs injected with FA + NC diet, D) In ovo probiotics-eggs injected with probiotics + NC diet, E) In ovo + in-water EO-eggs injected with EO and supplied EO via drinking water + NC diet. Birds were raised for 28 d in 8 replicate cages/treatment (6 birds/cage) and exposed to either a thermoneutral (24°C ± 0.2) or HS challenge (31°C) condition from d 21 to d 28. The in ovo delivered FA and EO treatments reduced (P < 0.001) hatchability by at least 26% compared to NC. Induced HS reduced (P < 0.001) total plasma protein, total antioxidant capacity, and villus width in the duodenum and jejunum. Independent of HS and compared to NC, the in ovo + in-water EO treatment resulted in (P < 0.05) at least a 15% increase in villus height: crypt depth across the 3 gut sections. The in ovo + in-water EO treatment also increased the relative mRNA expression of intestinal barrier-related genes (Claudin1,3,4, Occludin, Zona occludens-2, and Mucin 2). Under HS, the in ovo + in-water EO treatment recorded a 3.5-fold upregulation of amino acid transporter gene (SLC1A1), compared to NC. Subject to further hatchability optimization, the in ovo + in-water delivery of EO show potential to afford broiler chicken thermotolerance.

Citing Articles

Progress on the prevention of poultry Salmonella with natural medicines.

Zhang Y, Liu J, Pan Y, Shi K, Mai P, Li X Poult Sci. 2024; 104(1):104603.

PMID: 39631274 PMC: 11652915. DOI: 10.1016/j.psj.2024.104603.

References

Oladokun S, Koehler A, MacIsaac J, Ibeagha-Awemu E, Adewole D . Bacillus subtilis delivery route: effect on growth performance, intestinal morphology, cecal short-chain fatty acid concentration, and cecal microbiota in broiler chickens. Poult Sci. 2021; 100(3):100809. PMC: 7936168. DOI: 10.1016/j.psj.2020.10.063. View

Wen C, Chen Y, Leng Z, Ding L, Wang T, Zhou Y . Dietary betaine improves meat quality and oxidative status of broilers under heat stress. J Sci Food Agric. 2018; 99(2):620-623. DOI: 10.1002/jsfa.9223. View

Mohyuddin S, Qamar A, Hu C, Li Y, Chen S, Wen J . Terpinen4-ol inhibits heat stress induced inflammation in colonic tissue by Activating Occludin, Claudin-2 and TLR4/NF-κB signaling pathway. Int Immunopharmacol. 2021; 99:107727. DOI: 10.1016/j.intimp.2021.107727. View

Park I, Lee Y, Goo D, Zimmerman N, Smith A, Rehberger T . The effects of dietary Bacillus subtilis supplementation, as an alternative to antibiotics, on growth performance, intestinal immunity, and epithelial barrier integrity in broiler chickens infected with Eimeria maxima. Poult Sci. 2020; 99(2):725-733. PMC: 7587808. DOI: 10.1016/j.psj.2019.12.002. View

Ghazi S, Amjadian T, Norouzi S . Single and combined effects of vitamin C and oregano essential oil in diet, on growth performance, and blood parameters of broiler chicks reared under heat stress condition. Int J Biometeorol. 2014; 59(8):1019-24. DOI: 10.1007/s00484-014-0915-4. View

Salem H, Alqhtani A, Swelum A, Babalghith A, Melebary S, Soliman S . Heat stress in poultry with particular reference to the role of probiotics in its amelioration: An updated review. J Therm Biol. 2022; 108:103302. DOI: 10.1016/j.jtherbio.2022.103302. View

Capcarova M, Weiss J, Hrncar C, Kolesarova A, Pal G . Effect of Lactobacillus fermentum and Enterococcus faecium strains on internal milieu, antioxidant status and body weight of broiler chickens. J Anim Physiol Anim Nutr (Berl). 2010; 94(5):e215-24. DOI: 10.1111/j.1439-0396.2010.01010.x. View

Quinteiro-Filho W, Ribeiro A, Ferraz-de-Paula V, Pinheiro M, Sakai M, Sa L . Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens. Poult Sci. 2010; 89(9):1905-14. DOI: 10.3382/ps.2010-00812. View

Khalid A, Yasoob T, Zhang Z, Zhu X, Hang S . Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits. Environ Sci Pollut Res Int. 2022; 29(53):80952-80967. DOI: 10.1007/s11356-022-20737-6. View

10.

Song Z, Cheng K, Zhang L, Wang T . Dietary supplementation of enzymatically treated Artemisia annua could alleviate the intestinal inflammatory response in heat-stressed broilers. J Therm Biol. 2017; 69:184-190. DOI: 10.1016/j.jtherbio.2017.07.015. View

11.

Oladokun S, MacIsaac J, Rathgeber B, Adewole D . Essential Oil Delivery Route: Effect on Broiler Chicken's Growth Performance, Blood Biochemistry, Intestinal Morphology, Immune, and Antioxidant Status. Animals (Basel). 2021; 11(12). PMC: 8697888. DOI: 10.3390/ani11123386. View

12.

Gadde U, Kim W, Oh S, Lillehoj H . Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry: a review. Anim Health Res Rev. 2017; 18(1):26-45. DOI: 10.1017/S1466252316000207. View

13.

Tang S, Yin B, Xu J, Bao E . Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens. Oxid Med Cell Longev. 2018; 2018:7014126. PMC: 6218721. DOI: 10.1155/2018/7014126. View

14.

Song J, Xiao K, Ke Y, Jiao L, Hu C, Diao Q . Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Poult Sci. 2014; 93(3):581-8. DOI: 10.3382/ps.2013-03455. View

15.

Safdari-Rostamabad M, Hosseini-Vashan S, Perai A, Sarir H . Nanoselenium Supplementation of Heat-Stressed Broilers: Effects on Performance, Carcass Characteristics, Blood Metabolites, Immune Response, Antioxidant Status, and Jejunal Morphology. Biol Trace Elem Res. 2016; 178(1):105-116. DOI: 10.1007/s12011-016-0899-5. View

16.

Manafi M, Khalaji S, Hedayati M, Pirany N . Efficacy of Bacillus subtilis and bacitracin methylene disalicylate on growth performance, digestibility, blood metabolites, immunity, and intestinal microbiota after intramuscular inoculation with Escherichia coli in broilers. Poult Sci. 2017; 96(5):1174-1183. DOI: 10.3382/ps/pew347. View

17.

Chuang W, Liu C, Tsai C, Lin W, Chang S, Shih H . Evaluation of Waste Mushroom Compost as a Feed Supplement and Its Effects on the Fat Metabolism and Antioxidant Capacity of Broilers. Animals (Basel). 2020; 10(3). PMC: 7143042. DOI: 10.3390/ani10030445. View

18.

Xiong Y, Yin Q, Li J, He S . Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes. Animals (Basel). 2020; 10(3). PMC: 7142828. DOI: 10.3390/ani10030384. View

19.

Gouda A, Tolba S, Mahrose K . Influences of vitamin A, L-carnitine, and folic acid feeding on embryo and hatchling characteristics and general health status in ducks. Anim Biotechnol. 2021; 33(1):150-158. DOI: 10.1080/10495398.2020.1864389. View

20.

Li J, Xiao Y, Fan Q, Yang H, Yang C, Zhang G . Dietary Bacitracin Methylene Disalicylate Improves Growth Performance by Mediating the Gut Microbiota in Broilers. Antibiotics (Basel). 2022; 11(6). PMC: 9219630. DOI: 10.3390/antibiotics11060818. View