Solar-powered Multi-organism Symbiont Mimic System for Beyond Natural Synthesis of Polypeptides from CO and N

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2023 Mar 15

PMID 36921057

Authors

Wen Yu

Yue Zeng

Zenghao Wang

Shengpeng Xia

Zhiwen Yang

Weijian Chen

Yiming Huang

Fengting Lv

Haotian Bai

Shu Wang

Affiliations

Soon will be listed here.

Abstract

Developing artificial symbionts beyond natural synthesis limitations would bring revolutionary contributions to agriculture, medicine, environment, etc. Here, we initiated a solar-driven multi-organism symbiont, which was assembled by the CO fixation module of sp., N fixation module of , biofunctional polypeptides synthesis module of , and the electron transfer module of conductive cationic poly(fluorene--phenylene) derivative. The modular design broke the pathway to synthesize γ-polyglutamic acid (γ-PGA) using CO and N, attributing to the artificially constructed direct interspecific substance and electron transfer. So, the intracellular ATP and NADPH were enhanced by 69 and 30%, respectively, and the produced γ-PGA was enhanced by 104%. The strategy was further extended to produce a commercial antibiotic of bacitracin A. These achievements improve the selectivity and yield of functional polypeptides with one click by CO and N, and also provide an innovative strategy for creating photosynthetic systems on demand.

Citing Articles

Photosynthesis by nonphotosynthetic microorganisms via semiconductor photocatalysis.

Wang B, Shi L, Lu A mLife. 2025; 3(4):532-536.

PMID: 39744087 PMC: 11685834. DOI: 10.1002/mlf2.12156.

Organic Semiconducting Polymers for Augmenting Biosynthesis and Bioconversion.

Chen W, Lin H, Yu W, Huang Y, Lv F, Bai H JACS Au. 2024; 4(1):3-19.

PMID: 38274265 PMC: 10806880. DOI: 10.1021/jacsau.3c00576.

Open-endedness in synthetic biology: A route to continual innovation for biological design.

Stock M, Gorochowski T Sci Adv. 2024; 10(3):eadi3621.

PMID: 38241375 PMC: 11809665. DOI: 10.1126/sciadv.adi3621.

CO electrocatalytic reduction to ethylene and its application outlook in food science.

Ding Y, Dong Y, Ma M, Luo L, Wang X, Fang B iScience. 2023; 26(12):108434.

PMID: 38125022 PMC: 10730755. DOI: 10.1016/j.isci.2023.108434.

Lignin-derived carbon nanosheets boost electrochemical reductive amination of pyruvate to alanine.

Jia S, Tan X, Wu L, Zhao Z, Song X, Feng J iScience. 2023; 26(10):107776.

PMID: 37720096 PMC: 10502407. DOI: 10.1016/j.isci.2023.107776.

References

McCuskey S, Su Y, Leifert D, Moreland A, Bazan G . Living Bioelectrochemical Composites. Adv Mater. 2020; 32(24):e1908178. DOI: 10.1002/adma.201908178. View

Zhu C, Liu L, Yang Q, Lv F, Wang S . Water-soluble conjugated polymers for imaging, diagnosis, and therapy. Chem Rev. 2012; 112(8):4687-735. DOI: 10.1021/cr200263w. View

Wang T, Guo J, Peng Y, Lyu X, Liu B, Sun S . Light-induced mobile factors from shoots regulate rhizobium-triggered soybean root nodulation. Science. 2021; 374(6563):65-71. DOI: 10.1126/science.abh2890. View

Lewis N, Nocera D . Powering the planet: chemical challenges in solar energy utilization. Proc Natl Acad Sci U S A. 2006; 103(43):15729-35. PMC: 1635072. DOI: 10.1073/pnas.0603395103. View

Wegener G, Krukenberg V, Riedel D, Tegetmeyer H, Boetius A . Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria. Nature. 2015; 526(7574):587-90. DOI: 10.1038/nature15733. View

Sakimoto K, Wong A, Yang P . Self-photosensitization of nonphotosynthetic bacteria for solar-to-chemical production. Science. 2016; 351(6268):74-7. DOI: 10.1126/science.aad3317. View

Rode A, Carleton T, Delgado M, Greenstone M, Houser T, Hsiang S . Estimating a social cost of carbon for global energy consumption. Nature. 2021; 598(7880):308-314. DOI: 10.1038/s41586-021-03883-8. View

Alstrum-Acevedo J, Brennaman M, Meyer T . Chemical approaches to artificial photosynthesis. 2. Inorg Chem. 2005; 44(20):6802-27. DOI: 10.1021/ic050904r. View

Summers Z, Fogarty H, Leang C, Franks A, Malvankar N, Lovley D . Direct exchange of electrons within aggregates of an evolved syntrophic coculture of anaerobic bacteria. Science. 2010; 330(6009):1413-5. DOI: 10.1126/science.1196526. View

10.

Ha P, Lindemann S, Shi L, Dohnalkova A, Fredrickson J, Madigan M . Syntrophic anaerobic photosynthesis via direct interspecies electron transfer. Nat Commun. 2017; 8:13924. PMC: 5227917. DOI: 10.1038/ncomms13924. View

11.

Cai T, Sun H, Qiao J, Zhu L, Zhang F, Zhang J . Cell-free chemoenzymatic starch synthesis from carbon dioxide. Science. 2021; 373(6562):1523-1527. DOI: 10.1126/science.abh4049. View

12.

Lovley D . Syntrophy Goes Electric: Direct Interspecies Electron Transfer. Annu Rev Microbiol. 2017; 71:643-664. DOI: 10.1146/annurev-micro-030117-020420. View

13.

Tian G, Wang Q, Wei X, Ma X, Chen S . Glutamate dehydrogenase (RocG) in Bacillus licheniformis WX-02: Enzymatic properties and specific functions in glutamic acid synthesis for poly-γ-glutamic acid production. Enzyme Microb Technol. 2017; 99:9-15. DOI: 10.1016/j.enzmictec.2017.01.002. View

14.

Etschmann M, Bluemke W, Sell D, Schrader J . Biotechnological production of 2-phenylethanol. Appl Microbiol Biotechnol. 2002; 59(1):1-8. DOI: 10.1007/s00253-002-0992-x. View

15.

Zhou C, Wang F, Chen H, Li M, Qiao F, Liu Z . Selective Antimicrobial Activities and Action Mechanism of Micelles Self-Assembled by Cationic Oligomeric Surfactants. ACS Appl Mater Interfaces. 2016; 8(6):4242-9. DOI: 10.1021/acsami.5b12688. View

16.

Rodler A, Ueberbacher R, Beyer B, Jungbauer A . Calorimetry for studying the adsorption of proteins in hydrophobic interaction chromatography. Prep Biochem Biotechnol. 2019; 49(1):1-20. DOI: 10.1080/10826068.2018.1487852. View

17.

Lai B, Schneider H, Tschortner J, Schmid A, Kromer J . Technical-scale biophotovoltaics for long-term photo-current generation from Synechocystis sp. PCC6803. Biotechnol Bioeng. 2021; 118(7):2637-2648. DOI: 10.1002/bit.27784. View

18.

Miller T, Beneyton T, Schwander T, Diehl C, Girault M, McLean R . Light-powered CO fixation in a chloroplast mimic with natural and synthetic parts. Science. 2020; 368(6491):649-654. PMC: 7610767. DOI: 10.1126/science.aaz6802. View

19.

Gray H . Powering the planet with solar fuel. Nat Chem. 2011; 1(1):7. DOI: 10.1038/nchem.141. View

20.

Gai P, Yu W, Zhao H, Qi R, Li F, Liu L . Solar-Powered Organic Semiconductor-Bacteria Biohybrids for CO Reduction into Acetic Acid. Angew Chem Int Ed Engl. 2020; 59(18):7224-7229. DOI: 10.1002/anie.202001047. View