Distinct Aurora B Pools at the Inner Centromere and Kinetochore Have Different Contributions to Meiotic and Mitotic Chromosome Segregation

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2023 Mar 15

PMID 36920098

Authors

Gisela Cairo

Cora Greiwe

Gyu Ik Jung

Cecilia Blengini

Karen Schindler

Soni Lacefield

Affiliations

Soon will be listed here.

Abstract

Proper chromosome segregation depends on the establishment of bioriented kinetochore-microtubule attachments, which often requires multiple rounds of release and reattachment. Aurora B and C kinases phosphorylate kinetochore proteins to release tensionless attachments. Multiple pathways recruit Aurora B/C to the centromere and kinetochore. We studied how these pathways contribute to anaphase onset timing and correction of kinetochore-microtubule attachments in budding yeast meiosis and mitosis. We find that the pool localized by the Bub1/Bub3 pathway sets the normal duration of meiosis and mitosis, in differing ways. Our meiosis data suggests a model that disruption of this pathway leads to PP1 kinetochore localization, which dephosphorylates Cdc20 for premature anaphase onset. For error correction, the Bub1/Bub3 and COMA pathways are individually important in meiosis but compensatory in mitosis. Finally, we find that the haspin and Bub1/3 pathways function together to ensure error correction in mouse oogenesis. Our results suggest that each recruitment pathway localizes spatially distinct kinetochore-localized Aurora B/C pools that function differently between meiosis and mitosis.

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