Transcriptome, Intestinal Microbiome and Histomorphology Profiling of Differences in the Response of Chinese Sea Bass () to Infection

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Mar 13

PMID 36910190

Authors

Chao Pan

Yanran Zhu

Kaixin Cao

Juexian Li

Siyu Wang

Jiahua Zhu

Xiaoman Zeng

Heqian Zhang

Zhiwei Qin

Affiliations

Soon will be listed here.

Abstract

The Chinese sea bass () is an important aquaculture fish, but diseases caused by have led to severe economic losses to the aquaculture industry in recent years. To date, only a few studies have focused on the relationship between the intestinal immune response and changes in intestinal microbes by infection. Here, we report the transcriptome and intestinal changes in infected sea bass. Histopathological results showed that severe steatosis and vacuolation occurred in the liver and that the intestinal villi and mesentery were seriously affected after infection. By extracting total RNA from intestinal tissue and studying the transcriptome profile, 1,678 genes (1,013 upregulated and 665 downregulated) were identified as significantly differentially expressed genes (DEGs). These genes are involved in many immune-related signalling pathways, such as the NOD-like receptor, C-type lectin receptor, and Toll-like receptor signalling pathways. Moreover, the intestinal microbes of sea bass changed significantly after infection. Interestingly, at the genus level, there was an increase in , and as well as a decrease in and after infection. The results also indicated that some of the DEGs involved in the immune response were related to the genus level of intestinal microbiota. Finally, there was a relationship between gene expression patterns and the bacterial structure in the host intestine. Our study provides a reference for the study of the immune response and particular functions of intestinal microbes of sea bass after pathogen infection.

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