Interdisciplinary Biophysical Studies of Membrane Proteins Bacteriorhodopsin and Rhodopsin

Overview

Journal Biophys Rev

Publisher Springer

Specialty Biophysics

Date 2023 Mar 13

PMID 36909961

Authors

Karim Fahmy

Thomas P Sakmar

Affiliations

Soon will be listed here.

Abstract

The centenary of the birth of H. Gobind Khorana provides an auspicious opportunity to review the origins and evolution of parallel advances in biophysical methodology and molecular genetics technology used to study membrane proteins. Interdisciplinary work in the Khorana laboratory in the late 1970s and for the next three decades led to productive collaborations and fostered three subsequent scientific generations whose biophysical work on membrane proteins has led to detailed elucidation of the molecular mechanisms of energy transduction by the light-driven proton pump bacteriorhodopsin (bR) and signal transduction by the G protein-coupled receptor (GPCR) rhodopsin. This review will highlight the origins and advances of biophysical studies of membrane proteins made possible by the application of molecular genetics approaches to engineer site-specific alterations of membrane protein structures.

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