Effect of Immunomodulating Extract and Some Isolates from A.D. Poulsen Fruits on Diabetic Patients with Tuberculosis

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Mar 11

PMID 36903646

Authors

Muhammad Ilyas Y

Idin Sahidin

Asriullah Jabbar

Agung W M Yodha

Ajeng Diantini

Ivan Surya Pradipta

Riezki Amalia

Raden Maya Febrianti

Yuni Elsa Hadisaputri

Mohammad Ghozali

Euis Julaeha

Affiliations

Soon will be listed here.

Abstract

Diabetes mellitus (DM) is a disease easily complicated by tuberculosis (TB) due to impaired function of the innate immune response. The successes of the discovery of immunomodulatory compounds needs to be continued to introduce new insights into the innate immune response. In previous studies, plant compounds of A.D. Poulsen () were demonstrated to have potential as an immunomodulators. This study aims to isolate and identify the structure of the compounds of fruit that could effectively improve the function of the innate immune response in individuals with DM infected with TB. The isolation and purification of the compounds of the extract were carried out by radial chromatography (RC) and thin-layer chromatography (TLC). Identification of the isolated compound structures was determined by measuring the proton (H) and carbon (C) nuclear magnetic resonance (NMR). In vitro testing was performed on the immunomodulating activity of the extracts and isolated compounds on DM model macrophages infected with TB antigens. This study succeeded at isolating and identifying the structures of two isolate compounds, namely Sinaphyl alcohol diacetat (BER-1), and Ergosterol peroxide (BER-6). The two isolates were more effective as immunomodulators than the positive controls were, which differed significantly (* < 0.05) at the reducing interleukin-12 (IL-12) levels and Toll-like receptor-2 (TLR-2) protein expression and increasing the human leucocyte antigen-DR (HLA-DR) protein expression in DM infected with TB. The isolated compound was discovered in fruits, which has been reported to have the potential to be developed as an immunomodulatory agent. Follow-up testing to determine the mechanism and effectiveness of these compounds as immunomodulators for DM patients is required so that they are not susceptible to TB infection.

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