Protects Rats Against CCl-Induced Hepatic Injuries Through Modulation of an Unfolded Protein Response Pathway and Pro-Inflammatory Intermediates

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Mar 11

PMID 36903503

Authors

Riffat Batool

Muhammad Rashid Khan

Muhammad Umar Ijaz

Irum Naz

Afsheen Batool

Saima Ali

Zartash Zahra

Safia Gul

Mohammad N Uddin

Mohsin Kazi

Raees Khan

Affiliations

Soon will be listed here.

Abstract

Liver fibrosis is a major pathological feature of chronic liver disease and effective therapies are limited at present. The present study focuses on the hepatoprotective potential of . against carbon tetrachloride (CCl)-induced liver damage in rats. Analysis of methanol extract (LCM) using high-performance liquid chromatography (HPLC) revealed the presence of rutin, apigenin, catechin, caffeic acid and myricetin. CCl administration lowered ( < 0.01) the activities of antioxidant enzymes and reduced glutathione (GSH) content as well as soluble proteins, whereas the concentration of HO, nitrite and thiobarbituric acid reactive substances was higher in hepatic samples. In serum, the level of hepatic markers and total bilirubin was elevated followed by CCl administration. The expression of glucose-regulated protein (GRP78), x-box binding protein-1 total (XBP-1 t), x-box binding protein-1 spliced (XBP-1 s), x-box binding protein-1 unspliced (XBP-1 u) and glutamate-cysteine ligase catalytic subunit (GCLC) was enhanced in CCl-administered rats. Similarly, the expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemo attractant protein-1 (MCP-1) was strongly increased with CCl administration to rats. Co-administration of LCM along with CCl to rats lowered ( < 0.05) the expression of the above genes. Histopathology of the liver showed hepatocyte injury, leukocyte infiltration and damaged central lobules in CCl-treated rats. However, LCM administration to CCl-intoxicated rats restored the altered parameters towards the levels of control rats. These outcomes indicate the existence of antioxidant and anti-inflammatory constituents in the methanol extract of .

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