Cellular H MR Relaxation Times in Healthy and Cancer Three-Dimensional (3D) Breast Cell Culture

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Mar 11

PMID 36902163

Authors

Zuzanna Bober

Rafal Podgorski

David Aebisher

Grzegorz Cieslar

Aleksandra Kawczyk-Krupka

Dorota Bartusik-Aebisher

Affiliations

Soon will be listed here.

Abstract

Noninvasive measurements of H Magnetic Resonance Imaging (MR) relaxation times in a three-dimensional (3D) cell culture construct are presented. Trastuzumab was used as a pharmacological component delivered to the cells in vitro. The purpose of this study was to evaluate the Trastuzumab delivery by relaxation times in 3D cell cultures. The bioreactor has been designed and used for 3D cell cultures. Four bioreactors were prepared, two with normal cells and two with breast cancer cells. The relaxation times of HTB-125 and CRL 2314 cell cultures were determined. An immunohistochemistry (IHC) test was performed before MRI measurements to confirm the amount of HER2 protein in the CRL-2314 cancer cells. The results showed that the relaxation time of CRL2314 cells is lower than normal HTB-125 cells in both cases, before and after treatment. An analysis of the results showed that 3D culture studies have potential in evaluating treatment efficacy using relaxation times measurements with a field of 1.5 Tesla. The use H MRI relaxation times allows for the visualization of cell viability in response to treatment.

References

Koygun G, Demirel Kars M, Emsen A, Artac H, Aksoy F, Cakir M . Response to trastuzumab and investigation of expression profiles of matrix metalloproteinase-related proteins in primary breast cancer stem cells. Clin Exp Med. 2021; 21(3):447-456. DOI: 10.1007/s10238-021-00685-0. View

Zhang H, Peng Y . Current Biological, Pathological and Clinical Landscape of HER2-Low Breast Cancer. Cancers (Basel). 2023; 15(1). PMC: 9817919. DOI: 10.3390/cancers15010126. View

Chen B, Guo Z, Guo C, Mao Y, Qin Z, Ye D . Moderate cooling coprecipitation for extremely small iron oxide as a pH dependent T-MRI contrast agent. Nanoscale. 2020; 12(9):5521-5532. DOI: 10.1039/c9nr10397j. View

Arbab A, Liu W, Frank J . Cellular magnetic resonance imaging: current status and future prospects. Expert Rev Med Devices. 2006; 3(4):427-39. DOI: 10.1586/17434440.3.4.427. View

Luo X, Li J, Zhou C . [Preclinical application of MR and fluorescent dual-modality imaging combined with photothermal therapy in HER-2 positive breast cancer]. Zhonghua Zhong Liu Za Zhi. 2018; 40(8):587-593. DOI: 10.3760/cma.j.issn.0253-3766.2018.08.005. View

Perez A, Zhou J, Leach B, Xu H, Lister D, Adams S . Click-Ready Perfluorocarbon Nanoemulsion for F MRI and Multimodal Cellular Detection. ACS Nanosci Au. 2022; 2(2):102-110. PMC: 9026270. DOI: 10.1021/acsnanoscienceau.1c00016. View

Straathof R, Strijkers G, Nicolay K . Target-specific paramagnetic and superparamagnetic micelles for molecular MR imaging. Methods Mol Biol. 2011; 771:691-715. DOI: 10.1007/978-1-61779-219-9_34. View

Masthoff M, Freppon F, Zondler L, Wilken E, Wachsmuth L, Niemann S . Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking. EBioMedicine. 2021; 73:103670. PMC: 8581510. DOI: 10.1016/j.ebiom.2021.103670. View

Arbab A, Bashaw L, Miller B, Jordan E, Lewis B, Kalish H . Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging. Radiology. 2003; 229(3):838-46. DOI: 10.1148/radiol.2293021215. View

10.

Kim H, Lee G, Chang Y . Gadolinium as an MRI contrast agent. Future Med Chem. 2018; 10(6):639-661. DOI: 10.4155/fmc-2017-0215. View

11.

Yang H, Zhuang Y, Sun Y, Dai A, Shi X, Wu D . Targeted dual-contrast T1- and T2-weighted magnetic resonance imaging of tumors using multifunctional gadolinium-labeled superparamagnetic iron oxide nanoparticles. Biomaterials. 2011; 32(20):4584-93. DOI: 10.1016/j.biomaterials.2011.03.018. View

12.

Oude Engberink R, Blezer E, Hoff E, van der Pol S, van der Toorn A, Dijkhuizen R . MRI of monocyte infiltration in an animal model of neuroinflammation using SPIO-labeled monocytes or free USPIO. J Cereb Blood Flow Metab. 2007; 28(4):841-51. DOI: 10.1038/sj.jcbfm.9600580. View

13.

Hong H, Yang Y, Zhang Y, Cai W . Non-invasive cell tracking in cancer and cancer therapy. Curr Top Med Chem. 2010; 10(12):1237-48. PMC: 2916057. DOI: 10.2174/156802610791384234. View

14.

Wen L, Xia L, Guo X, Huang H, Wang F, Yang X . Multimodal Imaging Technology Effectively Monitors HER2 Expression in Tumors Using Trastuzumab-Coupled Organic Nanoparticles in Patient-Derived Xenograft Mice Models. Front Oncol. 2021; 11:778728. PMC: 8637767. DOI: 10.3389/fonc.2021.778728. View

15.

Liu L, Yin B, Shek K, Geng D, Lu Y, Wen J . Role of quantitative analysis of T2 relaxation time in differentiating benign from malignant breast lesions. J Int Med Res. 2018; 46(5):1928-1935. PMC: 5991255. DOI: 10.1177/0300060517721071. View

16.

Bartusik D, Tomanek B, Lattova E, Perreault H, Fallone G . Combined treatment of human MCF-7 breast carcinoma with antibody, cationic lipid and hyaluronic acid using ex vivo assays. J Pharm Biomed Anal. 2009; 51(1):192-201. DOI: 10.1016/j.jpba.2009.07.032. View

17.

Shapiro E, Skrtic S, Sharer K, Hill J, Dunbar C, Koretsky A . MRI detection of single particles for cellular imaging. Proc Natl Acad Sci U S A. 2004; 101(30):10901-6. PMC: 503717. DOI: 10.1073/pnas.0403918101. View

18.

Mouraviev V, Venkatraman T, Tovmasyan A, Kimura M, Tsivian M, Mouravieva V . Mn-porphyrins as novel molecular magnetic resonance imaging contrast agents. J Endourol. 2011; :1420-4. DOI: 10.1089/end.2011.0133. View

19.

Modo M, Ghuman H, Azar R, Krafty R, Badylak S, Hitchens T . Mapping the acute time course of immune cell infiltration into an ECM hydrogel in a rat model of stroke using F MRI. Biomaterials. 2022; 282:121386. DOI: 10.1016/j.biomaterials.2022.121386. View

20.

Brookes J, Redpath T, Gilbert F, Needham G, Murray A . Measurement of spin-lattice relaxation times with FLASH for dynamic MRI of the breast. Br J Radiol. 1996; 69(819):206-14. DOI: 10.1259/0007-1285-69-819-206. View