Optimization of Sperm Cryopreservation Formulation in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Mar 11

PMID 36901789

Authors

Le Chang

Chengpeng Lu

Junquan Zhu

Yiner Chen

Chunlin Wang

Changkao Mu

Congcong Hou

Affiliations

Soon will be listed here.

Abstract

is a very important marine economic species, and its aquaculture industry has been developing rapidly. However, the phenomenon of marine wild capture of and germplasm degradation has become increasingly serious. It is necessary to develop the artificial farming industry and carry out germplasm resource protection, for which sperm cryopreservation technology is an effective method. This research compared three methods (mesh-rubbing, trypsin digestion, and mechanical grinding) for acquiring free sperm, and the best method was mesh-rubbing. Then, the optimal cryopreservation conditions were selected, and the optimal formulation was sterile calcium-free artificial seawater, the optimal cryoprotectant was 20% glycerol, and the best equilibrium time was 15 min at 4 °C. The optimal cooling program was suspending the straws at 3.5 cm on the liquid nitrogen surface for 5 min and then storing them in liquid nitrogen. Finally, the sperm were thawed at 42 °C. However, the expression of sperm-related genes and the total enzymatic activities of frozen sperm were significantly decreased ( < 0.05), which showed that sperm cryopreservation damaged the sperm. Our study improves the sperm cryopreservation technology and the yield of aquaculture in Additionally, the study provides a certain technical basis for the establishment of a sperm cryopreservation library of crustaceans.

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