In Vivo and in Vitro Studies of a Propolis-enriched Silk Fibroin-gelatin Composite Nanofiber Wound Dressing

Overview

Journal Heliyon

Specialty Social Sciences

Date 2023 Mar 10

PMID 36895376

Authors

Pan Du

Xue Chen

Yang Chen

Jin Li

Yichi Lu

Xiaoxiao Li

Kai Hu

Junfeng Chen

Guozhong Lv

Affiliations

Soon will be listed here.

Abstract

In this study, electrospun nanofibers (NFs) used in trauma dressings were prepared using silk fibroin (SF) and gelatin (GT) as materials and highly volatile formic acid as the solvent, with three different concentrations of propolis extracts (EP), which were loaded through a simple process. The resulting samples were characterized by surface morphology, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), contact angle meter, water absorption, degradation rate, and mechanical property tests. The incorporation of propolis improved its antibacterial properties against , and , compared to those of the silk gelatin nanofiber material (SF/GT) alone. In vitro biocompatibility assays showed that SF/GT-1%EP had good cytocompatibility and hemocompatibility. In addition, it can also significantly promote the migration of L929 cells. SF/GT-1%EP was applied to a mouse model of full thickness skin defects, and it was found to significantly promote wound healing. These results indicate that the SF/GT-EP nanofiber material has good biocompatibility, migrating-promoting capability, antibacterial properties, and healing-promoting ability, providing a new idea for the treatment of full thickness skin defects.

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