Effects of Different Sperm Sources on the Clinical Outcomes of Oocyte Maturation Cycles Combined with Intracytoplasmic Sperm Injection

Overview

Journal Front Endocrinol (Lausanne)

Specialty Endocrinology

Date 2023 Mar 9

PMID 36891059

Authors

Jianhua Li

Jing Chen

Shuang Tian

Tingting Jiao

Jianye Wang

Yan Wei

Yanbin Cheng

Ye Xu

Ri-Cheng Chian

Youzhu Li

Shuiwen Zhang

Affiliations

Soon will be listed here.

Abstract

Objectives: To evaluate the embryonic developments and clinical outcomes of different sperm sources with cycles of intracytoplasmic sperm injection (ICSI) and maturation (IVM).

Methods: This retrospective study was approved by the hospital ethics committee and conducted in the hospital fertilization (IVF) clinic. From January 2005 to December 2018, 239 infertile couples underwent IVM-ICSI cycles and were divided into three groups according to different sperm sources. Group 1 comprised patients with percutaneous epididymal sperm aspiration (PESA; n = 62, 62 cycles), group 2 comprised patients with testicular sperm aspiration (TESA; n = 51, 51 cycles), and group 3 comprised patients with ejaculated sperm (n = 126, 126 cycles). We calculated the following outcomes: 1) outcomes per IVM-ICSI cycle: fertilization rate, cleavage rate, and embryo quality; 2) outcomes per embryo transfer cycle: endometrial thickness, implantation rate, biochemical pregnancy rate, clinical pregnancy rate, and live birth rate.

Results: There was no difference in basic characteristics among the three groups, such as the female partner's age, basal follicle-stimulating hormone (FSH), basal luteinizing hormone (LH), and antral follicle count (p > 0.1). There were no statistically significant differences according to the IVM-ICSI cycle among the three groups in fertilization rate, cleavage rate, and rate of good-quality embryos (p > 0.05). The results were similar among cycles regarding the number of transfer embryos and endometrial thickness per embryo transfer cycle among the three groups (p > 0.05). There were also similar clinical outcomes per embryo transfer cycle among the three groups, such as the biochemical pregnancy rate, clinical pregnancy rate, and live birth rate (p > 0.05).

Conclusions: Different sperm sources, percutaneous epididymal sperm aspiration, testicular sperm aspiration, and ejaculated sperm, do not affect the embryo and clinical outcomes after IVM-ICSI cycles.

Citing Articles

An overview of CFTR mutation profiles and assisted reproductive technology outcomes in Chinese patients with congenital obstructive azoospermia.

Wang M, Zhou J, Long R, Mao R, Gao L, Wang X J Assist Reprod Genet. 2023; 41(2):505-513.

PMID: 38114870 PMC: 10894795. DOI: 10.1007/s10815-023-03004-6.

References

Gonzalez-Ortega C, Pina-Aguilar R, Cancino-Villareal P, Gutierrez-Gutierrez A . Birth after human chorionic gonadotropin-primed oocyte maturation and fertilization with testicular sperm in a normo-ovulatory patient. J Hum Reprod Sci. 2016; 9(3):210-212. PMC: 5070405. DOI: 10.4103/0974-1208.192076. View

Li J, Chen J, Sun T, Zhang S, Jiao T, Chian R . Chromosome aneuploidy analysis in embryos derived from in vivo and in vitro matured human oocytes. J Transl Med. 2021; 19(1):416. PMC: 8501569. DOI: 10.1186/s12967-021-03080-1. View

Suganuma R, Yanagimachi R, Meistrich M . Decline in fertility of mouse sperm with abnormal chromatin during epididymal passage as revealed by ICSI. Hum Reprod. 2005; 20(11):3101-8. DOI: 10.1093/humrep/dei169. View

Bry-Gauillard H, Belin F, Vinolas C, Renoult-Pierre P, Massin N, Young J . Live birth after in-vitro maturation of oocytes in a patient with specific ovarian insufficiency caused by long-term mitotane treatment for adrenocortical carcinoma. Reprod Biomed Online. 2021; 44(2):304-309. DOI: 10.1016/j.rbmo.2021.10.004. View

Sharma U, Conine C, Shea J, Boskovic A, Derr A, Bing X . Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals. Science. 2016; 351(6271):391-396. PMC: 4888079. DOI: 10.1126/science.aad6780. View

Lim J, Yang S, Xu Y, Yoon S, Chian R . Selection of patients for natural cycle in vitro fertilization combined with in vitro maturation of immature oocytes. Fertil Steril. 2008; 91(4):1050-5. DOI: 10.1016/j.fertnstert.2008.01.066. View

Kawwass J, Chang J, Boulet S, Nangia A, Mehta A, Kissin D . Surgically acquired sperm use for assisted reproductive technology: trends and perinatal outcomes, USA, 2004-2015. J Assist Reprod Genet. 2018; 35(7):1229-1237. PMC: 6063834. DOI: 10.1007/s10815-018-1178-5. View

Sharma U, Sun F, Conine C, Reichholf B, Kukreja S, Herzog V . Small RNAs Are Trafficked from the Epididymis to Developing Mammalian Sperm. Dev Cell. 2018; 46(4):481-494.e6. PMC: 6103849. DOI: 10.1016/j.devcel.2018.06.023. View

Fernandes B, Dos Santos F, Bezerra L, de Brito P, de Macedo L, Silva A . Development of a microfluidic system structured on a modified polydimethylsiloxane device for the selection of bovine epididymal spermatozoa. Reprod Toxicol. 2022; 110:1-8. DOI: 10.1016/j.reprotox.2022.03.005. View

10.

Zhang S, Li J, Zhou G, Qin S, Xin N, Guo J . Infertility treatment for azoospermic patients with a history of infectious parotitis (mumps) using combined techniques. Hum Fertil (Camb). 2013; 16(4):266-8. DOI: 10.3109/14647273.2013.840392. View

11.

Akbari H, Mohammadi M . Effect of ovarian growth factors on ultra-structural maturation in frozen human immature oocytes after in vitro maturation: a comparative study. Reprod Health. 2022; 19(1):215. PMC: 9714011. DOI: 10.1186/s12978-022-01521-8. View

12.

Yu X, Lu S, Yuan M, Ma G, Li X, Zhang T . Does ICSI outcome in obstructive azoospermia differ according to the origin of retrieved spermatozoa or the cause of epididymal obstruction? A comparative study. Int Urol Nephrol. 2022; 54(12):3087-3095. PMC: 9606059. DOI: 10.1007/s11255-022-03350-x. View

13.

Li L, Liao H, Li M, Xiao J, Wu L . Comparative Clinical Study of Percutaneous Epididymal Sperm Aspiration and Testicular Biopsy in the Outcome of ICSI-Assisted Fertility Treatment in Patients with Obstructive Azoospermia. Front Surg. 2022; 9:901601. PMC: 9407240. DOI: 10.3389/fsurg.2022.901601. View

14.

Li J, Sun T, Zhang S, Jiao T, Cheng Y, Dong P . Effect of dominant follicle status at the time of retrieval on the clinical outcomes in natural cycle IVF combined with immature oocyte treatment. Aging (Albany NY). 2022; 14(11):4728-4738. PMC: 9217702. DOI: 10.18632/aging.204106. View

15.

Zhou D, Suzuki T, Asami M, Perry A . Caput Epididymidal Mouse Sperm Support Full Development. Dev Cell. 2019; 50(1):5-6. DOI: 10.1016/j.devcel.2019.05.012. View

16.

Morin S, Hanson B, Juneau C, Neal S, Landis J, Scott R . A comparison of the relative efficiency of ICSI and extended culture with epididymal sperm versus testicular sperm in patients with obstructive azoospermia. Asian J Androl. 2019; 22(2):222-226. PMC: 7155787. DOI: 10.4103/aja.aja_58_19. View

17.

Esteves S, Roque M, Bradley C, Garrido N . Reproductive outcomes of testicular versus ejaculated sperm for intracytoplasmic sperm injection among men with high levels of DNA fragmentation in semen: systematic review and meta-analysis. Fertil Steril. 2017; 108(3):456-467.e1. DOI: 10.1016/j.fertnstert.2017.06.018. View

18.

Ghazzawi I, Sarraf M, Taher M, Khalifa F . Comparison of the fertilizing capability of spermatozoa from ejaculates, epididymal aspirates and testicular biopsies using intracytoplasmic sperm injection. Hum Reprod. 1998; 13(2):348-52. DOI: 10.1093/humrep/13.2.348. View

19.

Dozortsev D, Neme R, Diamond M, Abdelmassih S, Abdelmassih V, Oliveira F . Embryos generated using testicular spermatozoa have higher developmental potential than those obtained using epididymal spermatozoa in men with obstructive azoospermia. Fertil Steril. 2006; 86(3):606-11. DOI: 10.1016/j.fertnstert.2006.01.036. View

20.

Javed A, Ramaiah M, Talkad M . ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively. Obstet Gynecol Sci. 2019; 62(6):429-437. PMC: 6856474. DOI: 10.5468/ogs.2019.62.6.429. View