ATP Synthase Interactome Analysis Identifies a New Subunit l as a Modulator of Permeability Transition Pore in Yeast

Overview

Journal Sci Rep

Specialty Science

Date 2023 Mar 7

PMID 36882574

Authors

Chiranjit Panja

Aneta Wiesyk

Katarzyna Niedzwiecka

Emilia Baranowska

Roza Kucharczyk

Affiliations

Soon will be listed here.

Abstract

The mitochondrial ATP synthase, an enzyme that synthesizes ATP and is involved in the formation of the mitochondrial mega-channel and permeability transition, is a multi-subunit complex. In S. cerevisiae, the uncharacterized protein Mco10 was previously found to be associated with ATP synthase and referred as a new 'subunit l'. However, recent cryo-EM structures could not ascertain Mco10 with the enzyme making questionable its role as a structural subunit. The N-terminal part of Mco10 is very similar to k/Atp19 subunit, which along with subunits g/Atp20 and e/Atp21 plays a major role in stabilization of the ATP synthase dimers. In our effort to confidently define the small protein interactome of ATP synthase we found Mco10. We herein investigate the impact of Mco10 on ATP synthase functioning. Biochemical analysis reveal in spite of similarity in sequence and evolutionary lineage, that Mco10 and Atp19 differ significantly in function. The Mco10 is an auxiliary ATP synthase subunit that only functions in permeability transition.

Citing Articles

The Haves and Have-Nots: The Mitochondrial Permeability Transition Pore across Species.

Frigo E, Tommasin L, Lippe G, Carraro M, Bernardi P Cells. 2023; 12(10).

PMID: 37408243 PMC: 10216546. DOI: 10.3390/cells12101409.

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