Direct Detection of Usual and Atypical Alleles on the Human Aldehyde Dehydrogenase-2 (ALDH2) Locus

Overview

Journal Am J Hum Genet

Publisher Cell Press

Specialty Genetics

Date 1987 Dec 1

PMID 3687946

Citations 20

Authors

L C Hsu

R E Bendel

A Yoshida

Affiliations

Soon will be listed here.

Abstract

A method for determining human mitochondrial aldehyde dehydrogenase (ALDH2) genotypes was developed. Two 21-base synthetic oligonucleotides, one complementary to the usual ALDH2(1) gene and the other complementary to the atypical ALDH2(2) gene, were used as specific probes for in-gel hybridization analysis of human genomic DNA from either peripheral blood cells or livers. Under appropriate hybridization conditions, these two probes can hybridize to their specific complementary alleles and thus allow the genotyping of the ALDH2 locus.

Citing Articles

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Effect of ALDH2 polymorphism on cancer risk in Asians: A meta-analysis.

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Clinical significance of ALDH2 rs671 polymorphism in esophageal cancer: evidence from 31 case-control studies.

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References

Blin N, Stafford D . A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976; 3(9):2303-8. PMC: 343085. DOI: 10.1093/nar/3.9.2303. View

Miyada C, Wallace R . Oligonucleotide hybridization techniques. Methods Enzymol. 1987; 154:94-107. DOI: 10.1016/0076-6879(87)54072-1. View

GOEDDE H, Agarwal D, Harada S . Alcohol metabolizing enzymes: studies of isozymes in human biopsies and cultured fibroblasts. Clin Genet. 1979; 16(1):29-33. DOI: 10.1111/j.1399-0004.1979.tb00845.x. View

GOEDDE H, Harada S, Agarwal D . Racial differences in alcohol sensitivity: a new hypothesis. Hum Genet. 1979; 51(3):331-4. DOI: 10.1007/BF00283404. View

Teng Y . Human liver aldehyde dehydrogenase in Chinese and Asiatic Indians: gene deletion and its possible implications in alcohol metabolism. Biochem Genet. 1981; 19(1-2):107-14. DOI: 10.1007/BF00486141. View

Impraim C, Wang G, Yoshida A . Structural mutation in a major human aldehyde dehydrogenase gene results in loss of enzyme activity. Am J Hum Genet. 1982; 34(6):837-41. PMC: 1685722. View

Conner B, Reyes A, Morin C, Itakura K, TEPLITZ R, Wallace R . Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides. Proc Natl Acad Sci U S A. 1983; 80(1):278-82. PMC: 393356. DOI: 10.1073/pnas.80.1.278. View

Ikawa M, Impraim C, Wang G, Yoshida A . Isolation and characterization of aldehyde dehydrogenase isozymes from usual and atypical human livers. J Biol Chem. 1983; 258(10):6282-7. View

Pirastu M, Kan Y, Cao A, Conner B, TEPLITZ R, Wallace R . Prenatal diagnosis of beta-thalassemia. Detection of a single nucleotide mutation in DNA. N Engl J Med. 1983; 309(5):284-7. DOI: 10.1056/NEJM198308043090506. View

10.

Lacy E, Roberts S, Evans E, Burtenshaw M, Costantini F . A foreign beta-globin gene in transgenic mice: integration at abnormal chromosomal positions and expression in inappropriate tissues. Cell. 1983; 34(2):343-58. DOI: 10.1016/0092-8674(83)90369-0. View

11.

Yoshida A, Wang G, Dave V . Determination of genotypes of human aldehyde dehydrogenase ALDH2 locus. Am J Hum Genet. 1983; 35(6):1107-16. PMC: 1685980. View

12.

Yoshida A . Determination of aldehyde dehydrogenase phenotypes using hair roots: re-examination. Hum Genet. 1984; 66(4):296-9. DOI: 10.1007/BF00287631. View

13.

Hsu L, Tani K, Fujiyoshi T, Kurachi K, Yoshida A . Cloning of cDNAs for human aldehyde dehydrogenases 1 and 2. Proc Natl Acad Sci U S A. 1985; 82(11):3771-5. PMC: 397869. DOI: 10.1073/pnas.82.11.3771. View

14.

Saiki R, Scharf S, Faloona F, Mullis K, Horn G, Erlich H . Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985; 230(4732):1350-4. DOI: 10.1126/science.2999980. View

15.

Saiki R, Bugawan T, Horn G, Mullis K, Erlich H . Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes. Nature. 1986; 324(6093):163-6. DOI: 10.1038/324163a0. View

16.

Greenfield N, Pietruszko R . Two aldehyde dehydrogenases from human liver. Isolation via affinity chromatography and characterization of the isozymes. Biochim Biophys Acta. 1977; 483(1):35-45. DOI: 10.1016/0005-2744(77)90005-5. View