Interaction of the Zika Virus with the Cytoplasmic Dynein-1

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2023 Mar 6

PMID 36879270

Authors

Dan Israel Zavala-Vargas

Giovani Visoso-Carbajal

Leticia Cedillo-Barron

Jessica Georgina Filisola-Villasenor

Romel Rosales-Ramirez

Juan E Ludert

Edgar Morales-Rios

Affiliations

Soon will be listed here.

Abstract

Zika virus (ZIKV) infection is a major public health threat, making the study of its biology a matter of great importance. By analyzing the viral-host protein interactions, new drug targets may be proposed. In this work, we showed that human cytoplasmic dynein-1 (Dyn) interacts with the envelope protein (E) of ZIKV. Biochemical evidence indicates that the E protein and the dimerization domain of the heavy chain of Dyn binds directly without dynactin or any cargo adaptor. Analysis of this interactions in infected Vero cells by proximity ligation assay suggest that the E-Dyn interaction is dynamic and finely tuned along the replication cycle. Altogether, our results suggest new steps in the replication cycle of the ZIKV for virion transport and indicate a suitable molecular target to modulate infection by ZIKV.

Citing Articles

The expanding clinical and genetic spectrum of DYNC1H1-related disorders.

Moller B, Becker L, Saffari A, Afenjar A, Coci E, Williamson R Brain. 2024; 148(2):597-612.

PMID: 38848546 PMC: 11788221. DOI: 10.1093/brain/awae183.

References

Marzo M, Griswold J, Ruff K, Buchmeier R, Fees C, Markus S . Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunction. Elife. 2019; 8. PMC: 6733598. DOI: 10.7554/eLife.47246. View

Barrows N, Campos R, Liao K, Prasanth K, Soto-Acosta R, Yeh S . Biochemistry and Molecular Biology of Flaviviruses. Chem Rev. 2018; 118(8):4448-4482. PMC: 5937540. DOI: 10.1021/acs.chemrev.7b00719. View

Kon T, Oyama T, Shimo-Kon R, Imamula K, Shima T, Sutoh K . The 2.8 Å crystal structure of the dynein motor domain. Nature. 2012; 484(7394):345-50. DOI: 10.1038/nature10955. View

Simmonds P, Becher P, Bukh J, Gould E, Meyers G, Monath T . ICTV Virus Taxonomy Profile: Flaviviridae. J Gen Virol. 2017; 98(1):2-3. PMC: 5370391. DOI: 10.1099/jgv.0.000672. View

Prange R, Streeck R . Novel transmembrane topology of the hepatitis B virus envelope proteins. EMBO J. 1995; 14(2):247-56. PMC: 398078. DOI: 10.1002/j.1460-2075.1995.tb06998.x. View

Krishnan M, Sukumaran B, Pal U, Agaisse H, Murray J, Hodge T . Rab 5 is required for the cellular entry of dengue and West Nile viruses. J Virol. 2007; 81(9):4881-5. PMC: 1900185. DOI: 10.1128/JVI.02210-06. View

Shrivastava N, Sripada S, Kaur J, Shah P, Cecilia D . Insights into the internalization and retrograde trafficking of Dengue 2 virus in BHK-21 cells. PLoS One. 2011; 6(10):e25229. PMC: 3186794. DOI: 10.1371/journal.pone.0025229. View

Jumper J, Evans R, Pritzel A, Green T, Figurnov M, Ronneberger O . Highly accurate protein structure prediction with AlphaFold. Nature. 2021; 596(7873):583-589. PMC: 8371605. DOI: 10.1038/s41586-021-03819-2. View

Mo Y, Salada B, Tambyah P . Zika virus-a review for clinicians. Br Med Bull. 2016; 119(1):25-36. DOI: 10.1093/bmb/ldw023. View

10.

Cortese M, Goellner S, Acosta E, Neufeldt C, Oleksiuk O, Lampe M . Ultrastructural Characterization of Zika Virus Replication Factories. Cell Rep. 2017; 18(9):2113-2123. PMC: 5340982. DOI: 10.1016/j.celrep.2017.02.014. View

11.

Brault J, Kudelko M, Vidalain P, Tangy F, Despres P, Pardigon N . The interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replication. Virology. 2011; 417(2):369-78. DOI: 10.1016/j.virol.2011.06.022. View

12.

Dai L, Song J, Lu X, Deng Y, Musyoki A, Cheng H . Structures of the Zika Virus Envelope Protein and Its Complex with a Flavivirus Broadly Protective Antibody. Cell Host Microbe. 2016; 19(5):696-704. DOI: 10.1016/j.chom.2016.04.013. View

13.

Xiang X, Qiu R, Yao X, Arst Jr H, Penalva M, Zhang J . Cytoplasmic dynein and early endosome transport. Cell Mol Life Sci. 2015; 72(17):3267-80. PMC: 4534323. DOI: 10.1007/s00018-015-1926-y. View

14.

Fernandez-Garcia M, Mazzon M, Jacobs M, Amara A . Pathogenesis of flavivirus infections: using and abusing the host cell. Cell Host Microbe. 2009; 5(4):318-28. DOI: 10.1016/j.chom.2009.04.001. View

15.

Schmidt H, Carter A . Review: Structure and mechanism of the dynein motor ATPase. Biopolymers. 2016; 105(8):557-67. PMC: 4879348. DOI: 10.1002/bip.22856. View

16.

Bos S, Viranaicken W, Turpin J, El-Kalamouni C, Roche M, Krejbich-Trotot P . The structural proteins of epidemic and historical strains of Zika virus differ in their ability to initiate viral infection in human host cells. Virology. 2018; 516:265-273. DOI: 10.1016/j.virol.2017.12.003. View

17.

Gullberg M, Gustafsdottir S, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C . Cytokine detection by antibody-based proximity ligation. Proc Natl Acad Sci U S A. 2004; 101(22):8420-4. PMC: 420409. DOI: 10.1073/pnas.0400552101. View

18.

Urnavicius L, Lau C, Elshenawy M, Morales-Rios E, Motz C, Yildiz A . Cryo-EM shows how dynactin recruits two dyneins for faster movement. Nature. 2018; 554(7691):202-206. PMC: 5988349. DOI: 10.1038/nature25462. View

19.

Wang X, Olberding K, White C, Li C . Bcl-2 proteins regulate ER membrane permeability to luminal proteins during ER stress-induced apoptosis. Cell Death Differ. 2010; 18(1):38-47. PMC: 2947581. DOI: 10.1038/cdd.2010.68. View

20.

Dodding M, Way M . Coupling viruses to dynein and kinesin-1. EMBO J. 2011; 30(17):3527-39. PMC: 3181490. DOI: 10.1038/emboj.2011.283. View