Overexpression Negatively Regulates Proanthocyanin Biosynthesis

Overview

Journal Front Plant Sci

Date 2023 Mar 6

PMID 36875575

Authors

Jing Guo

Yaqiong Wu

Tongli Wang

Yue Xin

Guibin Wang

Qi Zhou

Li-An Xu

Affiliations

Soon will be listed here.

Abstract

Flavonoids are important secondary metabolites with extensive pharmacological functions. L. (ginkgo) has attracted extensive attention because of its high flavonoid medicinal value. However, little is understood about ginkgo flavonol biosynthesis. Herein, we cloned the full-length gingko gene (1314 bp), which encodes a 363 amino acid protein that has a typical 2-oxoglutarate (2OG)-Fe(II) oxygenase region. Recombinant GbFLSa protein with a molecular mass of 41 kDa was expressed in BL21(DE3). The protein was localized to the cytoplasm. Moreover, proanthocyanins, including catechin, epicatechin, epigallocatechin and gallocatechin, were significantly less abundant in transgenic poplar than in nontransgenic (CK) plants. In addition, , and expression levels were significantly lower than those of their CK counterparts. GbFLSa thus encodes a functional protein that might negatively regulate proanthocyanin biosynthesis. This study helps elucidate the role of GbFLSa in plant metabolism and the potential molecular mechanism of flavonoid biosynthesis.

Citing Articles

Overview and Recent Progress on the Biosynthesis and Regulation of Flavonoids in L.

Guo J, Wang Y, Li J, Zhang J, Wu Y, Wang G Int J Mol Sci. 2023; 24(19).

PMID: 37834050 PMC: 10572177. DOI: 10.3390/ijms241914604.

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