RNA Sequencing-based Transcriptome Analysis of Granulosa Cells from Follicular Fluid: Genes Involved in Embryo Quality During in Vitro Fertilization and Embryo Transfer

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2023 Mar 1

PMID 36857405

Authors

Eun Jeong Yu

Won Yun Choi

Mi Seon Park

Jin Hee Eum

Dong Ryul Lee

Woo Sik Lee

Sang Woo Lyu

Sook Young Yoon

Affiliations

Soon will be listed here.

Abstract

Background: Granulosa cells play an important role in folliculogenesis, however, the role of RNA transcripts of granulosa cells in assessing embryo quality remains unclear. Therefore, we aims to investigate that RNA transcripts of granulosa cells be used to assess the probability of the embryonic developmental capacity.

Methods: This prospective cohort study was attempted to figure out the probability of the embryonic developmental capacity using RNA sequencing of granulosa cells. Granulosa cells were collected from 48 samples in good-quality embryo group and 79 in only poor- quality embryo group from women undergoing in vitro fertilization and embryo transfer treatment. Three samples from each group were used for RNA sequencing.

Results: 226 differentially expressed genes (DEGs) were related to high developmental competence of embryos. Gene Ontology enrichment analysis indicated that these DEGs were primarily involved in biological processes, molecular functions, and cellular components. Additionally, pathway analysis revealed that these DEGs were enriched in 13 Kyoto Encyclopedia of Genes and Genomes pathways. Reverse transcription quantitative polymerase chain reaction verified the differential expression of the 13 selected DEGs. Among them,10 genes were differently expressed in the poor-quality embryo group compared to good-quality embryo group, including CSF1R, CTSH, SERPINA1, CYP27A1, ITGB2, IL1β, TNF, TAB1, BCL2A1, and CCL4.

Conclusions: RNA sequencing data provide the support or confute granulosa expressed genes as non-invasive biomarkers for identifying the embryonic developmental capacity.

Citing Articles

Identification and Preliminary Analysis of Granulosa Cell Biomarkers to Predict Oocyte In Vitro Maturation Outcome in the Southern White Rhinoceros ().

Ruggeri E, Klohonatz K, Durrant B, Sirard M Animals (Basel). 2024; 14(23).

PMID: 39682503 PMC: 11640850. DOI: 10.3390/ani14233538.

Gene expression analysis of ovarian follicles and stromal cells in girls with Turner syndrome.

Peek R, van der Coelen S, Dolmans M Mol Hum Reprod. 2024; 30(12).

PMID: 39673521 PMC: 11655625. DOI: 10.1093/molehr/gaae043.

Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid in vitro.

Meng J, Chen X, Wang H, Mi Y, Zhou R, Zhang H Anim Biosci. 2024; 38(2):267-277.

PMID: 39210795 PMC: 11725750. DOI: 10.5713/ab.24.0363.

Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology.

Muraoka A, Yokoi A, Yoshida K, Kitagawa M, Asano-Inami E, Murakami M Commun Med (Lond). 2024; 4(1):33.

PMID: 38418565 PMC: 10902298. DOI: 10.1038/s43856-024-00460-8.

References

Young M, Wakefield M, Smyth G, Oshlack A . Gene ontology analysis for RNA-seq: accounting for selection bias. Genome Biol. 2010; 11(2):R14. PMC: 2872874. DOI: 10.1186/gb-2010-11-2-r14. View

Reinblatt S, Ishai L, Shehata F, Son W, Tulandi T, Almog B . Effects of ovarian endometrioma on embryo quality. Fertil Steril. 2011; 95(8):2700-2. DOI: 10.1016/j.fertnstert.2011.03.002. View

Cohen P, Nishimura K, Zhu L, Pollard J . Macrophages: important accessory cells for reproductive function. J Leukoc Biol. 1999; 66(5):765-72. DOI: 10.1002/jlb.66.5.765. View

Ratts V, Flaws J, Kolp R, Sorenson C, Tilly J . Ablation of bcl-2 gene expression decreases the numbers of oocytes and primordial follicles established in the post-natal female mouse gonad. Endocrinology. 1995; 136(8):3665-8. DOI: 10.1210/endo.136.8.7628407. View

Alfarawati S, Fragouli E, Colls P, Stevens J, Gutierrez-Mateo C, Schoolcraft W . The relationship between blastocyst morphology, chromosomal abnormality, and embryo gender. Fertil Steril. 2010; 95(2):520-4. DOI: 10.1016/j.fertnstert.2010.04.003. View

Duffy D, Seachord C, Dozier B . An ovulatory gonadotropin stimulus increases cytosolic phospholipase A2 expression and activity in granulosa cells of primate periovulatory follicles. J Clin Endocrinol Metab. 2005; 90(10):5858-65. DOI: 10.1210/jc.2005-0980. View

Lu C, Yan Z, Song X, Xu Y, Zheng X, Li R . Effect of exogenous gonadotropin on the transcriptome of human granulosa cells and follicular fluid hormone profiles. Reprod Biol Endocrinol. 2019; 17(1):49. PMC: 6591892. DOI: 10.1186/s12958-019-0489-4. View

Xu Y, Hutchison S, Hernandez-Ledezma J, Bogan R . Increased 27-hydroxycholesterol production during luteolysis may mediate the progressive decline in progesterone secretion. Mol Hum Reprod. 2017; 24(1):2-13. PMC: 6093402. DOI: 10.1093/molehr/gax061. View

Owerbach D, Pina L, Gabbay K . A 212-kb region on chromosome 6q25 containing the TAB2 gene is associated with susceptibility to type 1 diabetes. Diabetes. 2004; 53(7):1890-3. DOI: 10.2337/diabetes.53.7.1890. View

10.

Sirard M, Richard F, Blondin P, Robert C . Contribution of the oocyte to embryo quality. Theriogenology. 2005; 65(1):126-36. DOI: 10.1016/j.theriogenology.2005.09.020. View

11.

Takaesu G, Kishida S, Hiyama A, Yamaguchi K, Shibuya H, Irie K . TAB2, a novel adaptor protein, mediates activation of TAK1 MAPKKK by linking TAK1 to TRAF6 in the IL-1 signal transduction pathway. Mol Cell. 2000; 5(4):649-58. DOI: 10.1016/s1097-2765(00)80244-0. View

12.

Kim M, Park J, Jeon Y, Choe S, Lee H, Kim J . Correlation between Morphologic Grading and Euploidy Rates of Blastocysts, and Clinical Outcomes in In Vitro Fertilization Preimplantation Genetic Screening. J Korean Med Sci. 2019; 34(4):e27. PMC: 6345636. DOI: 10.3346/jkms.2019.34.e27. View

13.

Wyse B, Weizman N, Kadish S, Balakier H, Sangaralingam M, Librach C . Transcriptomics of cumulus cells - a window into oocyte maturation in humans. J Ovarian Res. 2020; 13(1):93. PMC: 7425158. DOI: 10.1186/s13048-020-00696-7. View

14.

Vassiliadis S, Relakis K, Papageorgiou A, Athanassakis I . Endometriosis and infertility: a multi-cytokine imbalance versus ovulation, fertilization and early embryo development. Clin Dev Immunol. 2005; 12(2):125-9. PMC: 2270735. DOI: 10.1080/17402520500125484. View

15.

Cummins J, Breen T, Harrison K, Shaw J, Wilson L, Hennessey J . A formula for scoring human embryo growth rates in in vitro fertilization: its value in predicting pregnancy and in comparison with visual estimates of embryo quality. J In Vitro Fert Embryo Transf. 1986; 3(5):284-95. DOI: 10.1007/BF01133388. View

16.

Aouacheria A, Brunet F, Gouy M . Phylogenomics of life-or-death switches in multicellular animals: Bcl-2, BH3-Only, and BNip families of apoptotic regulators. Mol Biol Evol. 2005; 22(12):2395-416. DOI: 10.1093/molbev/msi234. View

17.

Burnik Papler T, Vrtacnik Bokal E, Lovrecic L, Kopitar A, Maver A . No specific gene expression signature in human granulosa and cumulus cells for prediction of oocyte fertilisation and embryo implantation. PLoS One. 2015; 10(3):e0115865. PMC: 4359149. DOI: 10.1371/journal.pone.0115865. View

18.

Hamel M, Dufort I, Robert C, Leveille M, Leader A, Sirard M . Genomic assessment of follicular marker genes as pregnancy predictors for human IVF. Mol Hum Reprod. 2009; 16(2):87-96. DOI: 10.1093/molehr/gap079. View

19.

Koks S, Velthut A, Sarapik A, Altmae S, Reinmaa E, Schalkwyk L . The differential transcriptome and ontology profiles of floating and cumulus granulosa cells in stimulated human antral follicles. Mol Hum Reprod. 2009; 16(4):229-40. DOI: 10.1093/molehr/gap103. View

20.

Yamamoto Y, Kuwahara A, Taniguchi Y, Yamasaki M, Tanaka Y, Mukai Y . Tumor necrosis factor alpha inhibits ovulation and induces granulosa cell death in rat ovaries. Reprod Med Biol. 2015; 14(3):107-115. PMC: 4490172. DOI: 10.1007/s12522-014-0201-5. View