Microfluidics and Fluorescence Microscopy Protocol to Study the Response of C. elegans to Chemosensory Stimuli
Overview
Biomedical Engineering
Science
Affiliations
Here, we present a protocol to use microfluidics in combination with fluorescence microscopy to expose the C. elegans tail to chemosensory stimuli. We describe steps for the preparation of microfluidic chips and sample preparation through the sedation of C. elegans. We detail flow calibration and imaging of C. elegans through fluorescence microscopy to determine their molecular and/or cellular response to chemosensory stimuli. This protocol can also be applied to amphid neurons by inserting the worm in the chip head-first. For complete details on the use and execution of this protocol, please refer to Bruggeman et al. (2022)..
Multiplexed Microfluidic Platform for Parallel Bacterial Chemotaxis Assays.
Stehnach M, Henshaw R, Floge S, Guasto J Bio Protoc. 2024; 14(17):e5062.
PMID: 39282234 PMC: 11393045. DOI: 10.21769/BioProtoc.5062.