Cytotoxic Activity of the Ethyl Acetate Extract of Iraqi Carica Papaya Leaves in Breast and Lung Cancer Cell Lines

Overview

Journal Asian Pac J Cancer Prev

Specialty Oncology

Date 2023 Feb 28

PMID 36853308

Authors

Sura Basim

Ali A Kasim

Affiliations

Soon will be listed here.

Abstract

Methods: The ethyl acetate extract of Iraqi C. papaya leaves was prepared and tested for its phytochemical constitution. The 3-(4,5-dimethylthiazoline-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed in breast (MCF-7) and lung (A549) cells lines that were treated with different concentrations of ethyl acetate extract (3.125,6.25,12.5, 25, 50, and 100μg/ml). After 72 hrs of treatment, cell viability was evaluated.

Results: The ethyl acetate extract of C. papaya showed considerable cytotoxic activity in the MCF-7 and A549 cell lines. The activity was dose-dependent; The half-maximum inhibitory concentration (IC50) values were 22.74μg/ml and 8.674 μg/ml in the MCF-7 and A549 cell lines, respectively.

Conclusion: The ethyl acetate fraction of Iraqi C. papaya leaves has potential anticancer activity in lung and breast cancer.

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References

Mathur P, Sathishkumar K, Chaturvedi M, Das P, Sudarshan K, Santhappan S . Cancer Statistics, 2020: Report From National Cancer Registry Programme, India. JCO Glob Oncol. 2020; 6:1063-1075. PMC: 7392737. DOI: 10.1200/GO.20.00122. View

Kopustinskiene D, Jakstas V, Savickas A, Bernatoniene J . Flavonoids as Anticancer Agents. Nutrients. 2020; 12(2). PMC: 7071196. DOI: 10.3390/nu12020457. View

Abotaleb M, Samuel S, Varghese E, Varghese S, Kubatka P, Liskova A . Flavonoids in Cancer and Apoptosis. Cancers (Basel). 2019; 11(1). PMC: 6357032. DOI: 10.3390/cancers11010028. View

Mohammed M, Al-Taee M, Al-Shammari A . Caspase Dependent and Independent Anti-hematological Malignancy Activity of AMHA1 Attenuated Newcastle Disease Virus. Int J Mol Cell Med. 2020; 8(3):211-223. PMC: 7241843. DOI: 10.22088/IJMCM.BUMS.8.3.211. View

Tolosa L, Donato M, Gomez-Lechon M . General Cytotoxicity Assessment by Means of the MTT Assay. Methods Mol Biol. 2015; 1250:333-48. DOI: 10.1007/978-1-4939-2074-7_26. View

Roleira F, Tavares-da-Silva E, Varela C, Costa S, Silva T, Garrido J . Plant derived and dietary phenolic antioxidants: anticancer properties. Food Chem. 2015; 183:235-58. DOI: 10.1016/j.foodchem.2015.03.039. View

Pandey S, Walpole C, Cabot P, Shaw P, Batra J, Hewavitharana A . Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer. Biomed Pharmacother. 2017; 89:515-523. DOI: 10.1016/j.biopha.2017.02.050. View

Abdullah S, Al-Shammari A, Lateef S . Attenuated measles vaccine strain have potent oncolytic activity against Iraqi patient derived breast cancer cell line. Saudi J Biol Sci. 2020; 27(3):865-872. PMC: 7042618. DOI: 10.1016/j.sjbs.2019.12.015. View

Elekofehinti O, Iwaloye O, Olawale F, Ariyo E . Saponins in Cancer Treatment: Current Progress and Future Prospects. Pathophysiology. 2022; 28(2):250-272. PMC: 8830467. DOI: 10.3390/pathophysiology28020017. View

10.

Greenwell M, Rahman P . Medicinal Plants: Their Use in Anticancer Treatment. Int J Pharm Sci Res. 2015; 6(10):4103-4112. PMC: 4650206. DOI: 10.13040/IJPSR.0975-8232.6(10).4103-12. View

11.

Al-Hussaniy H . The Effect of MicroRNA-409-3p for Treatment and Response to Tumor Proliferation of Lung Cancer Cell Lines (In Vitro). Asian Pac J Cancer Prev. 2022; 23(9):3151-3156. DOI: 10.31557/APJCP.2022.23.9.3151. View

12.

Li H, Wang Z, Liu Y . [Review in the studies on tannins activity of cancer prevention and anticancer]. Zhong Yao Cai. 2003; 26(6):444-8. View

13.

Al-Shammari A, Abdullah A, Allami Z, Yaseen N . 2-Deoxyglucose and Newcastle Disease Virus Synergize to Kill Breast Cancer Cells by Inhibition of Glycolysis Pathway Through Glyceraldehyde3-Phosphate Downregulation. Front Mol Biosci. 2019; 6:90. PMC: 6777003. DOI: 10.3389/fmolb.2019.00090. View

14.

Otsuki N, Dang N, Kumagai E, Kondo A, Iwata S, Morimoto C . Aqueous extract of Carica papaya leaves exhibits anti-tumor activity and immunomodulatory effects. J Ethnopharmacol. 2009; 127(3):760-7. DOI: 10.1016/j.jep.2009.11.024. View

15.

Menger L, Vacchelli E, Adjemian S, Martins I, Ma Y, Shen S . Cardiac glycosides exert anticancer effects by inducing immunogenic cell death. Sci Transl Med. 2012; 4(143):143ra99. DOI: 10.1126/scitranslmed.3003807. View

16.

Nguyen T, Shaw P, Parat M, Hewavitharana A . Anticancer activity of Carica papaya: a review. Mol Nutr Food Res. 2012; 57(1):153-64. DOI: 10.1002/mnfr.201200388. View

17.

Al-Ziaydi A, Al-Shammari A, Hamzah M, Kadhim H, Jabir M . Newcastle disease virus suppress glycolysis pathway and induce breast cancer cells death. Virusdisease. 2020; 31(3):341-348. PMC: 7458979. DOI: 10.1007/s13337-020-00612-z. View

18.

Siegel R, Ma J, Zou Z, Jemal A . Cancer statistics, 2014. CA Cancer J Clin. 2014; 64(1):9-29. DOI: 10.3322/caac.21208. View

19.

Zuhrotun Nisa F, Astuti M, Murdiati A, Haryana S . Anti-proliferation and Apoptosis Induction of Aqueous Leaf Extract of Carica papaya L. on Human Breast Cancer Cells MCF-7. Pak J Biol Sci. 2017; 20(1):36-41. DOI: 10.3923/pjbs.2017.36.41. View

20.

Khan H, Saeedi M, Nabavi S, Mubarak M, Bishayee A . Glycosides from Medicinal Plants as Potential Anticancer Agents: Emerging Trends Towards Future Drugs. Curr Med Chem. 2018; 26(13):2389-2406. DOI: 10.2174/0929867325666180403145137. View