QsvR and OpaR Coordinately Repress Biofilm Formation by

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Feb 27

PMID 36846774

Authors

Miaomiao Zhang

Xingfan Xue

Xue Li

Qimin Wu

Tingting Zhang

Wenhui Yang

Lingfei Hu

Dongsheng Zhou

Renfei Lu

Yiquan Zhang

Affiliations

Soon will be listed here.

Abstract

Mature biofilm formation by requires exopolysaccharide (EPS), type IV pili, and capsular polysaccharide (CPS). Production of each is strictly regulated by various control pathways including quorum sensing (QS) and bis-(3'-5')-cyclic di-GMP (c-di-GMP). QsvR, an AraC-type regulator, integrates into the QS regulatory cascade direct control of the transcription of the master QS regulators, AphA and OpaR. Deletion of in wild-type or mutant backgrounds altered the biofilm formation by , suggesting that QsvR may coordinate with OpaR to control biofilm formation. Herein, we demonstrated both QsvR and OpaR repressed biofilm-associated phenotypes, c-di-GMP metabolism, and the formation of translucent (TR) colonies. QsvR restored the biofilm-associated phenotypic changes caused by mutation, and vice versa. In addition, QsvR and OpaR worked coordinately to regulate the transcription of EPS-associated genes, type IV pili genes, CPS genes and c-di-GMP metabolism-related genes. These results demonstrated how QsvR works with the QS system to regulate biofilm formation by precisely controlling the transcription of multiple biofilm formation-associated genes in .

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