From Prediction to Function: Current Practices and Challenges Towards the Functional Characterization of Type III Effectors

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Feb 27

PMID 36846751

Authors

Joren De Ryck

Petra Van Damme

Sofie Goormachtig

Affiliations

Soon will be listed here.

Abstract

The type III secretion system (T3SS) is a well-studied pathogenicity determinant of many bacteria through which effectors (T3Es) are translocated into the host cell, where they exercise a wide range of functions to deceive the host cell's immunity and to establish a niche. Here we look at the different approaches that are used to functionally characterize a T3E. Such approaches include host localization studies, virulence screenings, biochemical activity assays, and large-scale omics, such as transcriptomics, interactomics, and metabolomics, among others. By means of the phytopathogenic species complex (RSSC) as a case study, the current advances of these methods will be explored, alongside the progress made in understanding effector biology. Data obtained by such complementary methods provide crucial information to comprehend the entire function of the effectome and will eventually lead to a better understanding of the phytopathogen, opening opportunities to tackle it.

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