Associations Between Oral Human Herpesvirus-6 and -7 and Periodontal Conditions in Older Adults

Overview

Journal Life (Basel)

Specialty Biology

Date 2023 Feb 25

PMID 36836681

Authors

Natsuki Hamada

Hideo Shigeishi

Iori Oka

Mio Sasaki

Honami Kitasaki

Mariko Nakamura

Kanako Yano

Chia-Hsin Wu

Yoshino Kaneyasu

Tomoko Maehara

Masaru Sugiyama

Kouji Ohta

Affiliations

Soon will be listed here.

Abstract

Background: The associations between oral human herpesvirus-6 (HHV-6) and HHV-7, periodontal conditions, and lifestyle-related diseases, such as hypertension, diabetes, and dyslipidemia, have not been fully investigated in older adults.

Methods: Seventy-four older patients who visited Hiroshima University Hospital were enrolled. Tongue swab samples were employed, and a real-time polymerase chain reaction was performed to detect HHV-6 and HHV-7 DNA. Dental plaque accumulation, probing pocket depth, and bleeding on probing (BOP) (i.e., a sign of periodontal inflammation) were examined. The periodontal inflamed surface area (PISA) value (i.e., an indicator of the severity of periodontitis) was also examined.

Results: Of the 74 participants, one participant (1.4%) was HHV-6 DNA-positive and 36 participants (48.6%) were HHV-7 DNA-positive. A significant association between HHV-7 DNA and probing depth was found ( = 0.04). The HHV-7 DNA-positive participants had a higher positive rate of a ≥6-mm periodontal pocket with BOP (25.0%) than the HHV-7 DNA-negative participants (7.9%). Additionally, the HHV-7 DNA-positive participants had a higher PISA value than the HHV-7 DNA-negative participants. However, there was no significant association between HHV-7 and the PISA value ( = 0.82). No significant association was found between HHV-7 and lifestyle-related diseases ( > 0.05).

Conclusions: Oral HHV-7 infection is associated with a deep periodontal pocket.

Citing Articles

The functions of herpesvirus shuttling proteins in the virus lifecycle.

Cao H, Wang M, Cheng A, Tian B, Yang Q, Ou X Front Microbiol. 2025; 16:1515241.

PMID: 39973925 PMC: 11837949. DOI: 10.3389/fmicb.2025.1515241.

Prevalence of Epstein-Barr Virus, Cytomegalovirus, and Periodontopathic Bacteria in Patients With Periodontitis: A Case-Control Study.

Herrero-Sanchez A, Haroyan-Darbinyan E Clin Exp Dent Res. 2025; 11(1):e70084.

PMID: 39916439 PMC: 11803075. DOI: 10.1002/cre2.70084.

Multiple functions of the herpesvirus UL14 gene product in viral infection.

Wan J, Wang M, Cheng A, Zhang W, Yang Q, Tian B Front Microbiol. 2024; 15:1483022.

PMID: 39507342 PMC: 11537914. DOI: 10.3389/fmicb.2024.1483022.

Human herpesvirus 7 meningitis in an adolescent with normal immune function: A case report.

Zhen Y, Yang J, Liao P World J Clin Cases. 2024; 12(18):3636-3643.

PMID: 38983391 PMC: 11229909. DOI: 10.12998/wjcc.v12.i18.3636.

Prevalence of oral species and their association with dental plaque accumulation and periodontal inflammation in middle‑aged and older people.

Shigeishi H, Hamada N, Kaneyasu Y, Niitani Y, Takemoto T, Ohta K Biomed Rep. 2024; 20(6):99.

PMID: 38765863 PMC: 11099601. DOI: 10.3892/br.2024.1787.

References

Frenkel N, Schirmer E, Wyatt L, Katsafanas G, Roffman E, Danovich R . Isolation of a new herpesvirus from human CD4+ T cells. Proc Natl Acad Sci U S A. 1990; 87(2):748-52. PMC: 53343. DOI: 10.1073/pnas.87.2.748. View

Salahuddin S, Ablashi D, Markham P, Josephs S, Sturzenegger S, Kaplan M . Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science. 1986; 234(4776):596-601. DOI: 10.1126/science.2876520. View

Zerr D, Meier A, Selke S, Frenkel L, Huang M, Wald A . A population-based study of primary human herpesvirus 6 infection. N Engl J Med. 2005; 352(8):768-76. DOI: 10.1056/NEJMoa042207. View

Fujisaki H, Tanaka-Taya K, Tanabe H, Hara T, Miyoshi H, Okada S . Detection of human herpesvirus 7 (HHV-7) DNA in breast milk by polymerase chain reaction and prevalence of HHV-7 antibody in breast-fed and bottle-fed children. J Med Virol. 1998; 56(3):275-9. DOI: 10.1002/(sici)1096-9071(199811)56:3<275::aid-jmv17>3.0.co;2-d. View

Suga S, Yoshikawa T, Nagai T, Asano Y . Clinical features and virological findings in children with primary human herpesvirus 7 infection. Pediatrics. 1997; 99(3):E4. DOI: 10.1542/peds.99.3.e4. View

Voumvourakis K, Fragkou P, Kitsos D, Foska K, Chondrogianni M, Tsiodras S . Human herpesvirus 6 infection as a trigger of multiple sclerosis: an update of recent literature. BMC Neurol. 2022; 22(1):57. PMC: 8845292. DOI: 10.1186/s12883-022-02568-7. View

Eliassen E, Lum E, Pritchett J, Ongradi J, Krueger G, Crawford J . Human Herpesvirus 6 and Malignancy: A Review. Front Oncol. 2018; 8:512. PMC: 6277865. DOI: 10.3389/fonc.2018.00512. View

Vasques Raposo J, Sarmento D, Barbosa da Silva Pinto R, Lopes A, Gallottini M, Tozetto-Mendoza T . Longitudinal study on oral shedding of human betaherpesviruses 6 and 7 in renal transplant recipients reveals active replication. J Oral Microbiol. 2020; 12(1):1785801. PMC: 7482732. DOI: 10.1080/20002297.2020.1785801. View

Ablashi D, Berneman Z, Kramarsky B, Whitman Jr J, Asano Y, Pearson G . Human herpesvirus-7 (HHV-7): current status. Clin Diagn Virol. 1995; 4(1):1-13. DOI: 10.1016/0928-0197(95)00005-s. View

10.

Curtis M, Diaz P, Van Dyke T . The role of the microbiota in periodontal disease. Periodontol 2000. 2020; 83(1):14-25. DOI: 10.1111/prd.12296. View

11.

Dworzanski J, Drop B, Kliszczewska E, Strycharz-Dudziak M, Polz-Dacewicz M . Prevalence of Epstein-Barr virus, human papillomavirus, cytomegalovirus and herpes simplex virus type 1 in patients with diabetes mellitus type 2 in south-eastern Poland. PLoS One. 2019; 14(9):e0222607. PMC: 6759159. DOI: 10.1371/journal.pone.0222607. View

12.

Shigeishi H, Oka I, Su C, Hamada N, Nakamura M, Nishimura R . Prevalence of oral Epstein-Barr virus and Porphyromonas gingivalis and their association with periodontal inflamed surface area: A cross-sectional study. Medicine (Baltimore). 2022; 101(43):e31282. PMC: 10662808. DOI: 10.1097/MD.0000000000031282. View

13.

Nesse W, Abbas F, van der Ploeg I, Spijkervet F, Dijkstra P, Vissink A . Periodontal inflamed surface area: quantifying inflammatory burden. J Clin Periodontol. 2008; 35(8):668-73. DOI: 10.1111/j.1600-051X.2008.01249.x. View

14.

Imai K, Ogata Y . How Does Epstein-Barr Virus Contribute to Chronic Periodontitis?. Int J Mol Sci. 2020; 21(6). PMC: 7139403. DOI: 10.3390/ijms21061940. View

15.

Miranda-Silva W, Knebel F, Tozetto-Mendozo T, Palmieri M, da Fonseca F, Camargo A . Herpesviruses in the oral cavity of patients subjected to allogeneic hematopoietic stem cell transplantation and its relationship with oral mucositis. Clin Oral Investig. 2020; 24(10):3597-3608. DOI: 10.1007/s00784-020-03234-3. View

16.

Slots J, Slots H . Periodontal herpesvirus morbidity and treatment. Periodontol 2000. 2019; 79(1):210-220. DOI: 10.1111/prd.12241. View

17.

Jauhiainen M, Xu M, Pyoria L, Atula T, Aro K, Markkanen A . The presence of herpesviruses in malignant but not in benign or recurrent pleomorphic adenomas. Tumour Biol. 2021; 43(1):249-259. DOI: 10.3233/TUB-211519. View

18.

Nakamura M, Shigeishi H, Cheng-Yih S, Sugiyama M, Ohta K . Oral human cytomegalovirus prevalence and its relationships with periodontitis and Porphyromonas gingivalis in Japanese adults: a cross-sectional study. J Appl Oral Sci. 2020; 28:e20200501. PMC: 7793530. DOI: 10.1590/1678-7757-2020-00501. View

19.

Yamamoto K, Yoshikawa T, Okamoto S, Yamaki K, Shimokata K, Nishiyama Y . HHV-6 and 7 DNA loads in lung tissues collected from patients with interstitial pneumonia. J Med Virol. 2004; 75(1):70-5. DOI: 10.1002/jmv.20239. View

20.

Rotola A, Cassai E, Farina R, Caselli E, Gentili V, Lazzarotto T . Human herpesvirus 7, Epstein-Barr virus and human cytomegalovirus in periodontal tissues of periodontally diseased and healthy subjects. J Clin Periodontol. 2008; 35(10):831-7. DOI: 10.1111/j.1600-051X.2008.01301.x. View