Diversity of Mycoviruses Present in Strains of Binucleate and Multinucleate , Causal Agents for Potato Stem Canker or Black Scurf

Overview

Journal J Fungi (Basel)

Date 2023 Feb 25

PMID 36836328

Authors

Yuting Li

Naibo Yang

Tongyu Mu

Xuehong Wu

Can Zhao

Affiliations

Soon will be listed here.

Abstract

In this study, the diversity of putative mycoviruses present in 66 strains of binucleate (BNR, including anastomosis group (AG)-A, AG-Fa, AG-K, and AG-W) and 192 strains of multinucleate (MNR, including AG-1-IA, AG-2-1, AG-3 PT, AG-4HGI, AG-4HGII, AG-4HGIII, and AG-5), which are the causal agents of potato stem canker or black scurf, was studied using metatranscriptome sequencing. The number of contigs related to mycoviruses identified from BNR and MNR was 173 and 485, respectively. On average, each strain of BNR accommodated 2.62 putative mycoviruses, while each strain of MNR accommodated 2.53 putative mycoviruses. Putative mycoviruses detected in both BNR and MNR contained positive single-stranded RNA (+ssRNA), double-stranded RNA (dsRNA), and negative single-stranded RNA (-ssRNA) genomes, with +ssRNA genome being the prevalent nucleic acid type (82.08% in BNR and 75.46% in MNR). Except for 3 unclassified, 170 putative mycoviruses found in BNR belonged to 13 families; excluding 33 unclassified, 452 putative mycoviruses found in MNR belonged to 19 families. Through genome organization, multiple alignments, and phylogenetic analyses, 4 new parititviruses, 39 novel mitoviruses, and 4 new hypoviruses with nearly whole genome were detected in the 258 strains of BNR and MNR.

Citing Articles

Metatranscriptomic Sequencing of Sheath Blight-Associated Isolates of Revealed Multi-Infection by Diverse Groups of RNA Viruses.

Urzo M, Guinto T, Eusebio-Cope A, Budot B, Yanoria M, Jonson G Viruses. 2024; 16(7).

PMID: 39066314 PMC: 11281561. DOI: 10.3390/v16071152.

Unveiling the Genetic Tapestry: Exploring AG-3 Anastomosis Groups in Potato Crops across Borders.

Naqvi S, Abbas A, Farhan M, Kiran R, Hassan Z, Mehmood Y Plants (Basel). 2024; 13(5).

PMID: 38475561 PMC: 10934047. DOI: 10.3390/plants13050715.

Anastomosis Groups and Mycovirome of Isolates Causing Sugar Beet Root and Crown Rot and Their Sensitivity to Flutolanil, Thifluzamide, and Pencycuron.

Zhao C, Li S, Ma Z, Wang W, Gao L, Han C J Fungi (Basel). 2023; 9(5).

PMID: 37233256 PMC: 10219533. DOI: 10.3390/jof9050545.

References

Tran T, Li H, Nguyen D, Jones M, Wylie S . Co-Infection with Three Mycoviruses Stimulates Growth of a Isolate on Nutrient Medium, but Does Not Induce Hypervirulence in a Natural Host. Viruses. 2019; 11(1). PMC: 6356717. DOI: 10.3390/v11010089. View

Roossinck M . Plant virus metagenomics: biodiversity and ecology. Annu Rev Genet. 2012; 46:359-69. DOI: 10.1146/annurev-genet-110711-155600. View

Zhu J, Zhu H, Gao B, Zhou Q, Zhong J . Diverse, Novel Mycoviruses From the Virome of a Hypovirulent Strain. Front Plant Sci. 2018; 9:1738. PMC: 6277794. DOI: 10.3389/fpls.2018.01738. View

Zhang H, Xie J, Fu Y, Cheng J, Qu Z, Zhao Z . A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement. Mol Plant. 2020; 13(10):1420-1433. DOI: 10.1016/j.molp.2020.08.016. View

Carling D, Brainard K, Virgen-Calleros G, Olalde-Portugal V . First Report of Rhizoctonia solani AG-7 on Potato in Mexico. Plant Dis. 2019; 82(1):127. DOI: 10.1094/PDIS.1998.82.1.127C. View

Shi M, Lin X, Tian J, Chen L, Chen X, Li C . Redefining the invertebrate RNA virosphere. Nature. 2016; 540(7634):539-543. DOI: 10.1038/nature20167. View

Li S, Li Y, Hu C, Han C, Zhou T, Zhao C . Full genome sequence of a new mitovirus from the phytopathogenic fungus Rhizoctonia solani. Arch Virol. 2020; 165(7):1719-1723. DOI: 10.1007/s00705-020-04664-w. View

Hao F, Ding T, Wu M, Zhang J, Yang L, Chen W . Two Novel Hypovirulence-Associated Mycoviruses in the Phytopathogenic Fungus : Molecular Characterization and Suppression of Infection Cushion Formation. Viruses. 2018; 10(5). PMC: 5977247. DOI: 10.3390/v10050254. View

Linder-Basso D, Dynek J, Hillman B . Genome analysis of Cryphonectria hypovirus 4, the most common hypovirus species in North America. Virology. 2005; 337(1):192-203. DOI: 10.1016/j.virol.2005.03.038. View

10.

Kotta-Loizou I . Mycoviruses and their role in fungal pathogenesis. Curr Opin Microbiol. 2021; 63:10-18. DOI: 10.1016/j.mib.2021.05.007. View

11.

Li Y, Xu P, Zhang L, Chen W, Ren Z, Yang G . Complete nucleotide sequence of a novel mycovirus infecting Rhizoctonia fumigata AG-Ba isolate C-314 Baishi. Arch Virol. 2022; 167(3):959-963. DOI: 10.1007/s00705-021-05269-7. View

12.

Vainio E, Chiba S, Ghabrial S, Maiss E, Roossinck M, Sabanadzovic S . ICTV Virus Taxonomy Profile: Partitiviridae. J Gen Virol. 2017; 99(1):17-18. PMC: 5882087. DOI: 10.1099/jgv.0.000985. View

13.

Li Y, Sun Y, Xu P, Zhang L, Chen Z, Niu Y . A novel alphapartitivirus from binucleate Rhizoctonia fumigata AG-Ba isolate C-314 Baishi. Arch Virol. 2021; 167(1):255-259. DOI: 10.1007/s00705-021-05270-0. View

14.

Yang Y, Wu X . First Report of Potato Stem Canker Caused by Binucleate Rhizoctonia AG-A in Jilin Province, China. Plant Dis. 2019; 97(9):1246. DOI: 10.1094/PDIS-10-12-0912-PDN. View

15.

Milgroom M, Cortesi P . Biological control of chestnut blight with hypovirulence: a critical analysis. Annu Rev Phytopathol. 2004; 42:311-38. DOI: 10.1146/annurev.phyto.42.040803.140325. View

16.

Neang S, Bincader S, Rangsuwan S, Keawmanee P, Rin S, Salaipeth L . Omnipresence of Partitiviruses in Rice Aggregate Sheath Spot Symptom-Associated Fungal Isolates from Paddies in Thailand. Viruses. 2021; 13(11). PMC: 8625198. DOI: 10.3390/v13112269. View

17.

Zhong J, Chen D, Lei X, Zhu H, Zhu J, Gao B . Detection and characterization of a novel Gammapartitivirus in the phytopathogenic fungus Colletotrichum acutatum strain HNZJ001. Virus Res. 2014; 190:104-9. DOI: 10.1016/j.virusres.2014.05.028. View

18.

Hu Z, Wu S, Cheng J, Fu Y, Jiang D, Xie J . Molecular characterization of two positive-strand RNA viruses co-infecting a hypovirulent strain of Sclerotinia sclerotiorum. Virology. 2014; 464-465:450-459. DOI: 10.1016/j.virol.2014.07.007. View

19.

Lyu R, Zhang Y, Tang Q, Li Y, Cheng J, Fu Y . Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani. Arch Virol. 2017; 163(2):515-520. DOI: 10.1007/s00705-017-3627-3. View

20.

Li P, Zhang H, Chen X, Qiu D, Guo L . Molecular characterization of a novel hypovirus from the plant pathogenic fungus Fusarium graminearum. Virology. 2015; 481:151-60. DOI: 10.1016/j.virol.2015.02.047. View