Conidium Specific Polysaccharides in

Overview

Journal J Fungi (Basel)

Date 2023 Feb 25

PMID 36836270

Authors

Zhonghua Liu

Isabel Valsecchi

Remy A Le Meur

Catherine Simenel

J Inaki Guijarro

Catherine Comte

Laetitia Muszkieta

Isabelle Mouyna

Bernard Henrissat

Vishukumar Aimanianda

Jean-Paul Latge

Thierry Fontaine

Affiliations

Soon will be listed here.

Abstract

Earlier studies have shown that the outer layers of the conidial and mycelial cell walls of are different. In this work, we analyzed the polysaccharidome of the resting conidial cell wall and observed major differences within the mycelium cell wall. Mainly, the conidia cell wall was characterized by (i) a smaller amount of α-(1,3)-glucan and chitin; (ii) a larger amount of β-(1,3)-glucan, which was divided into alkali-insoluble and water-soluble fractions, and (iii) the existence of a specific mannan with side chains containing galactopyranose, glucose, and N-acetylglucosamine residues. An analysis of cell wall gene mutants suggested that members of the fungal GH-72 transglycosylase family play a crucial role in the conidia cell wall β-(1,3)-glucan organization and that α-(1,6)-mannosyltransferases of GT-32 and GT-62 families are essential to the polymerization of the conidium-associated cell wall mannan. This specific mannan and the well-known galactomannan follow two independent biosynthetic pathways.

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