Genome-Wide Characterization and Analysis of BHLH Transcription Factors Related to Anthocyanin Biosynthesis in ('Gantong 1')

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Feb 25

PMID 36834907

Authors

Xue Gong

Tengfei Shen

XiuQi Li

Hanbin Lin

Caihui Chen

Huihu Li

Zhaoxiang Wu

Qiaoli Liu

Meng Xu

Bo Zhang

Yongda Zhong

Affiliations

Soon will be listed here.

Abstract

is one of the most commonly used tree species in landscaping. Improving its ornamental traits, particularly bark and leaf colors, is one of the key breeding goals. The basic helix-loop-helix (bHLH) transcription factors (TFs) are crucial in controlling anthocyanin biosynthesis in many plants. However, their role in remains largely unknown. In this study, we identified 150 bHLH TFs () using natural mutant 'Gantong 1', which has unusual bark and leaf colors. Phylogenetic analysis revealed that 150 CcbHLHs were divided into 26 subfamilies which shared similar gene structures and conserved motifs. According to the protein homology analysis, we identified four candidate CcbHLHs that were highly conserved compared to the TT8 protein in . These TFs are potentially involved in anthocyanin biosynthesis in . RNA-seq analysis revealed specific expression patterns of in different tissue types. Furthermore, we verified expression patterns of seven (, , , , , , and ) in various tissue types at different growth stages using qRT-PCR. This study opens a new avenue for subsequent research on anthocyanin biosynthesis regulated by TFs in .

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