Anaerobic Storage Completely Removes Suspected Fungal Pathogens but Increases Antibiotic Resistance Gene Levels in Swine Wastewater High in Sulfonamides

Overview

Journal Int J Environ Res Public Health

Publisher MDPI

Specialties Environmental Health
Public Health

Date 2023 Feb 25

PMID 36833839

Authors

Xinyue Zhao

Mengjie Zhang

Zhilin Sun

Huabao Zheng

Qifa Zhou

Affiliations

Soon will be listed here.

Abstract

Wastewater storage before reuse is regulated in some countries. Investigations of pathogens and antibiotic resistance genes (ARGs) during wastewater storage are necessary for lowering the risks for wastewater reuse but are still mostly lacking. This study aimed to investigate pathogens, including harmful plant pathogens, and ARGs during 180 d of swine wastewater (SWW) storage in an anaerobic storage experiment. The contents of total organic carbon and total nitrogen in SWW were found to consistently decrease with the extension of storage time. Bacterial abundance and fungal abundance significantly decreased with storage time, which may be mainly attributed to nutrient loss during storage and the long period of exposure to a high level (4653.2 μg/L) of sulfonamides in the SWW, which have an inhibitory effect. It was found that suspected bacterial pathogens (e.g., spp., spp., spp., spp., and spp.) and sulfonamide-resistant genes , , , and tended to persist and even become enriched during SWW storage. Interestingly, some suspected plant fungal species (e.g., spp., spp. and spp.) were detected in SWW. Fungi in the SWW, including threatening fungal pathogens, were completely removed after 60 d of anaerobic storage, indicating that storage could lower the risk of using SWW in crop production. The results clearly indicate that storage time is crucial for SWW properties, and long periods of anaerobic storage could lead to substantial nutrient loss and enrichment of bacterial pathogens and ARGs in SWW.

References

Kiu R, Hall L . An update on the human and animal enteric pathogen Clostridium perfringens. Emerg Microbes Infect. 2018; 7(1):141. PMC: 6079034. DOI: 10.1038/s41426-018-0144-8. View

Shi F, Zhao X, Cheng Q, Lin H, Zheng H, Zhou Q . High-Energy-Density Organic Amendments Enhance Soil Health. Int J Environ Res Public Health. 2022; 19(19). PMC: 9566092. DOI: 10.3390/ijerph191912212. View

Daigger G . Oxygen and carbon requirements for biological nitrogen removal processes accomplishing nitrification, nitritation, and anammox. Water Environ Res. 2014; 86(3):204-9. DOI: 10.2175/106143013x13807328849459. View

Xinjie W, Xin N, Qilu C, Ligen X, Yuhua Z, Qifa Z . Vetiver and co-culture for the removal of nutrients and ecological inactivation of pathogens in swine wastewater. J Adv Res. 2019; 20:71-78. PMC: 6562367. DOI: 10.1016/j.jare.2019.05.004. View

Ma L, Geiser D, Proctor R, Rooney A, ODonnell K, Trail F . Fusarium pathogenomics. Annu Rev Microbiol. 2013; 67:399-416. DOI: 10.1146/annurev-micro-092412-155650. View

Lu P, Guo L, Wang Z, Li B, Li J, Li Y . A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew. Nat Commun. 2020; 11(1):680. PMC: 6997164. DOI: 10.1038/s41467-020-14294-0. View

Krishnasamy V, Otte J, Silbergeld E . Antimicrobial use in Chinese swine and broiler poultry production. Antimicrob Resist Infect Control. 2015; 4:17. PMC: 4412119. DOI: 10.1186/s13756-015-0050-y. View

Prokhorova A, Kainuma M, Hiyane R, Boerner S, Goryanin I . Concurrent treatment of raw and aerated swine wastewater using an electrotrophic denitrification system. Bioresour Technol. 2020; 322:124508. DOI: 10.1016/j.biortech.2020.124508. View

Zhang M, Liu Y, Zhao J, Liu W, Chen J, Zhang Q . Variations of antibiotic resistome in swine wastewater during full-scale anaerobic digestion treatment. Environ Int. 2021; 155:106694. DOI: 10.1016/j.envint.2021.106694. View

10.

Guo X, Sun C, Lin R, Xia A, Huang Y, Zhu X . Effects of foam nickel supplementation on anaerobic digestion: Direct interspecies electron transfer. J Hazard Mater. 2020; 399:122830. DOI: 10.1016/j.jhazmat.2020.122830. View

11.

Du R, Cao S, Zhang H, Li X, Peng Y . Flexible Nitrite Supply Alternative for Mainstream Anammox: Advances in Enhancing Process Stability. Environ Sci Technol. 2020; 54(10):6353-6364. DOI: 10.1021/acs.est.9b06265. View

12.

Chao A, Bunge J . Estimating the number of species in a stochastic abundance model. Biometrics. 2002; 58(3):531-9. DOI: 10.1111/j.0006-341x.2002.00531.x. View

13.

Lackner S, Gilbert E, Vlaeminck S, Joss A, Horn H, van Loosdrecht M . Full-scale partial nitritation/anammox experiences--an application survey. Water Res. 2014; 55:292-303. DOI: 10.1016/j.watres.2014.02.032. View

14.

Topp E, Scott A, Lapen D, Lyautey E, Duriez P . Livestock waste treatment systems for reducing environmental exposure to hazardous enteric pathogens: some considerations. Bioresour Technol. 2008; 100(22):5395-8. DOI: 10.1016/j.biortech.2008.11.001. View

15.

Tao C, Hsu B, Ji W, Hsu T, Kao P, Hsu C . Evaluation of five antibiotic resistance genes in wastewater treatment systems of swine farms by real-time PCR. Sci Total Environ. 2014; 496:116-121. DOI: 10.1016/j.scitotenv.2014.07.024. View

16.

Hu H, Li X, Wu S, Yang C . Sustainable livestock wastewater treatment via phytoremediation: Current status and future perspectives. Bioresour Technol. 2020; 315:123809. DOI: 10.1016/j.biortech.2020.123809. View

17.

Holmes D, Bond D, ONeil R, Reimers C, Tender L, Lovley D . Microbial communities associated with electrodes harvesting electricity from a variety of aquatic sediments. Microb Ecol. 2004; 48(2):178-90. DOI: 10.1007/s00248-003-0004-4. View

18.

Chu L, Ding P, Ding M . Pilot-scale microaerobic hydrolysis-acidification and anoxic-oxic processes for the treatment of petrochemical wastewater. Environ Sci Pollut Res Int. 2021; 28(41):58677-58687. DOI: 10.1007/s11356-021-14810-9. View

19.

Zhu N, Jin H, Ye X, Liu W, Li D, Shah G . Fate and driving factors of antibiotic resistance genes in an integrated swine wastewater treatment system: From wastewater to soil. Sci Total Environ. 2020; 721:137654. DOI: 10.1016/j.scitotenv.2020.137654. View

20.

He L, He L, Liu Y, Zhang M, Zhao J, Zhang Q . Microbial diversity and antibiotic resistome in swine farm environments. Sci Total Environ. 2019; 685:197-207. DOI: 10.1016/j.scitotenv.2019.05.369. View