Characterization of NDM-5-Producing Strains Isolated from Pediatric Patients with Bloodstream Infections in a Chinese Hospital

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 Feb 25

PMID 36833447

Authors

Lili Huang

Hongye Hu

Chen Xu

Mi Zhou

Yuanyuan Li

Yunbing Li

Shuyan Wu

Ning Dong

Affiliations

Soon will be listed here.

Abstract

() bloodstream infections (BSIs) are among the most predominant causes of death in infants and children worldwide. NDM-5 (New Delhi Metallo-lactamase-5) is responsible for one of the main mechanisms of carbapenem resistance in . To analyze the phenotypic and genomic characteristics of NDM-5-producing from bloodstream infections (BSIs), a total of 114 strains was collected from a children's hospital in Jiangsu province, China. Eight -carrying strains were identified which were all carbapenem-resistant and carried diverse antimicrobial resistance genes apart from . They belonged to six distinct sequence types (STs) and serotypes including one each for ST38/O7:H8, ST58/O?:H37, ST131/O25:H4, ST156/O11:H25 and ST361/O9:H30 and three strains are originating from a single clone belonging to ST410/O?:H9. Apart from , the strains isolated from BSIs also carried other β-lactamase genes, including ( = 4), ( = 2), ( = 3), ( = 1), ( = 4) and ( = 5). The genes were located on three different types of plasmids, which were IncFII/I1 ( = 1), IncX3 ( = 4) and IncFIA/FIB/FII/Q1 ( = 3). The former two types were conjugatively transferable at frequencies of 10 and 10, respectively. The dissemination of NDM-producing strains, which exhibit resistance to the last-line antibiotics, carbapenems, may increase the muti-antimicrobial resistance burden among BSIs and further threaten public health.

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