Wild Mushroom Extract As Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2023 Feb 25

PMID 36831990

Authors

Andre O Santos

Vanessa E Abrantes-Coutinho

Simone Morais

Thiago M B F Oliveira

Affiliations

Soon will be listed here.

Abstract

mushroom biomass contains a lectin, ABL, with remarkable specificity for lactose biorecognition; in this work, this feature was explored to develop a photoelectrochemical biosensor. The high lectin activity found in saline extracts of this macrofungus (640 HU mL), even at critical pH values (4-10) and temperatures (20-100 °C), allowed its direct use as an ABL source. Theoretical and experimental evidence revealed favorable electrostatic and biocompatible conditions to immobilize ABL on a poly(methylene blue)/fluorine-doped tin oxide-coated glass platform, giving rise to the ABL/PMB/FTO biosensor. The conducting polymer added further photoactivity to the device, allowing the identification of lectin-carbohydrate interactions with even greater sensitivity. The dose-response curves studied by electrochemical impedance spectroscopy showed a sigmoidal profile that was well-fitted by Hill's equation, expanding the working dynamic range (15-540 nmol L lactose; 20.2 pmol L detection limit) and avoiding undesirable sample dilution or preconcentration procedures. Under the optimized photoelectrochemical conditions, the ABL/PMB/FTO biosensor showed remarkable signal stability, accuracy, specificity, and selectivity to analyze lactose in commercial food products. This research raises interest in ABL-based biosensors and the added value of the crude extract toward the development of greener and more sustainable biotechnological approaches.

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