Insights into Transcriptomic Differences in Ovaries Between Lambs and Adult Sheep After Superovulation Treatment

Overview

Journal Animals (Basel)

Date 2023 Feb 25

PMID 36830452

Authors

Qingwei Wang

Xiaofei Guo

Dawei Yao

Biao Wang

YuPeng Li

Jinlong Zhang

Xiaosheng Zhang

Affiliations

Soon will be listed here.

Abstract

Superovulation technology shows a great potential for shortening breeding time. Using the juvenile superovulation technology, juvenile animals can generate more follicles than adult animals. By sequencing using high-throughput methods, we studied and described differentially expressed (DE) long non-coding RNA (lncRNAs) and messenger RNAs (mRNAs) in the ovaries of young and adult sheep. Herein, 242 DE lncRNAs and 3150 DE mRNAs were screened. Through GO and KEGG analyses, we obtained genes related to ovarian/follicle development and ovulation in DE mRNAs, including , , , , , and ; through lncRNA-mRNA correlation analysis, we found that genes associated with ovarian/follicle development or ovulation include: XR_003585520.1, MSTRG.15652.1, XR_003588840.1, and their paired genes , , and . We observed a synergistic effect between and . may play a leading role compared with . The induced in lambs is higher than in adult sheep, showing more sensitivity to LH. The release of the oocytes was stimulated. Among the three lncRNAs, we found that XR_003588840.1 was significantly different and might perform a regulatory role in ovarian/follicle growth or ovulation.

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