Pulsed Electric Fields (PEF) and Accelerated Solvent Extraction (ASE) for Valorization of Red () and Camarote () Shrimp Side Streams: Antioxidant and HPLC Evaluation of the Carotenoid Astaxanthin Recovery

Overview

Journal Antioxidants (Basel)

Date 2023 Feb 25

PMID 36829965

Authors

Ana Cristina De Aguiar Saldanha Pinheiro

Francisco J Marti-Quijal

Francisco J Barba

Ana M Benitez-Gonzalez

Antonio J Melendez-Martinez

Juan Manuel Castagnini

Silvia Tappi

Pietro Rocculi

Affiliations

Soon will be listed here.

Abstract

Shrimp side streams represent an important natural source of astaxanthin. Optimization of the astaxanthin extraction process from shrimp side streams is of great importance for the valorization of crustacean side streams and the development of astaxanthin-related products. The combined and independent effects of two innovative extraction technologies (pulsed electric fields (PEFs) and accelerated solvent extraction (ASE)) alone and/or combined in a sequential step, using two different solvents on astaxanthin extraction from two shrimp species, were evaluated. Astaxanthin content in the extracts of shrimp side streams was determined by both spectrophotometric and HPLC assays, being the determination of the carotenoid profiles performed by HPLC analysis. Compared to a solvent extraction control procedure, the astaxanthin content was increased after ASE and PEF treatments, for both shrimp species, independently of the solvent used. The highest recovery (585.90 µg/g) was obtained for the species , with the solvent DMSO when PEF and ASE were combined, while the increase in antioxidant capacity varied depending on the solvent used. HPLC analysis of the samples revealed the presence of unesterified (all-E) astaxanthin, four unesterified Z isomers of astaxanthin and many unresolved astaxanthin esters. Both technologies are useful tools to recover antioxidant valuable carotenoids such as astaxanthin from shrimp side streams.

Citing Articles

Anti-Inflammatory, Antithrombotic, and Antioxidant Properties of Amphiphilic Lipid Bioactives from Shrimp.

Tsoupras A, Cholidis P, Kranas D, Galouni E, Ofrydopoulou A, Efthymiopoulos P Pharmaceuticals (Basel). 2025; 18(1.

PMID: 39861088 PMC: 11768413. DOI: 10.3390/ph18010025.

Extraction and Synthesis of Typical Carotenoids: Lycopene, β-Carotene, and Astaxanthin.

Jiang Y, Ye J, Hu Y, Zhang J, Li W, Zhou X Molecules. 2024; 29(19).

PMID: 39407479 PMC: 11478001. DOI: 10.3390/molecules29194549.

Polyphenols: Secondary Metabolites with a Biological Impression.

Bolat E, Saritas S, Duman H, Eker F, Akdasci E, Karav S Nutrients. 2024; 16(15).

PMID: 39125431 PMC: 11314462. DOI: 10.3390/nu16152550.

Sustainable Green Extraction of Carotenoid Pigments: Innovative Technologies and Bio-Based Solvents.

Moron-Ortiz A, Mapelli-Brahm P, Melendez-Martinez A Antioxidants (Basel). 2024; 13(2).

PMID: 38397837 PMC: 10886214. DOI: 10.3390/antiox13020239.

References

Sachindra N, Bhaskar N, Mahendrakar N . Recovery of carotenoids from shrimp waste in organic solvents. Waste Manag. 2005; 26(10):1092-8. DOI: 10.1016/j.wasman.2005.07.002. View

Holtin K, Kuehnle M, Rehbein J, Schuler P, Nicholson G, Albert K . Determination of astaxanthin and astaxanthin esters in the microalgae Haematococcus pluvialis by LC-(APCI)MS and characterization of predominant carotenoid isomers by NMR spectroscopy. Anal Bioanal Chem. 2009; 395(6):1613-22. DOI: 10.1007/s00216-009-2837-2. View

Pinheiro A, Marti-Quijal F, Barba F, Tappi S, Rocculi P . Innovative Non-Thermal Technologies for Recovery and Valorization of Value-Added Products from Crustacean Processing By-Products-An Opportunity for a Circular Economy Approach. Foods. 2021; 10(9). PMC: 8465042. DOI: 10.3390/foods10092030. View

Stinco C, Benitez-Gonzalez A, Melendez-Martinez A, Hernanz D, Vicario I . Simultaneous determination of dietary isoprenoids (carotenoids, chlorophylls and tocopherols) in human faeces by Rapid Resolution Liquid Chromatography. J Chromatogr A. 2018; 1583:63-72. DOI: 10.1016/j.chroma.2018.11.010. View

Breithaupt D . Identification and quantification of astaxanthin esters in shrimp (Pandalus borealis) and in a microalga (Haematococcus pluvialis) by liquid chromatography-mass spectrometry using negative ion atmospheric pressure chemical ionization. J Agric Food Chem. 2004; 52(12):3870-5. DOI: 10.1021/jf049780b. View

Magalhaes L, Segundo M, Reis S, Lima J . Methodological aspects about in vitro evaluation of antioxidant properties. Anal Chim Acta. 2008; 613(1):1-19. DOI: 10.1016/j.aca.2008.02.047. View

Mezzomo N, Maestri B, dos Santos R, Maraschin M, Ferreira S . Pink shrimp (P. brasiliensis and P. paulensis) residue: influence of extraction method on carotenoid concentration. Talanta. 2011; 85(3):1383-91. DOI: 10.1016/j.talanta.2011.06.018. View

Melendez-Martinez A, Stinco C, Liu C, Wang X . A simple HPLC method for the comprehensive analysis of cis/trans (Z/E) geometrical isomers of carotenoids for nutritional studies. Food Chem. 2013; 138(2-3):1341-50. DOI: 10.1016/j.foodchem.2012.10.067. View

Milde J, Elstner E, Grassmann J . Synergistic effects of phenolics and carotenoids on human low-density lipoprotein oxidation. Mol Nutr Food Res. 2007; 51(8):956-61. DOI: 10.1002/mnfr.200600271. View

10.

Gulzar S, Benjakul S . Impact of pretreatment and atmosphere on quality of lipids extracted from cephalothorax of Pacific white shrimp by ultrasonic assisted process. Food Chem. 2019; 309:125732. DOI: 10.1016/j.foodchem.2019.125732. View

11.

Prameela K, Venkatesh K, Immandi S, Kasturi A, Rama Krishna C, Murali Mohan C . Next generation nutraceutical from shrimp waste: The convergence of applications with extraction methods. Food Chem. 2017; 237:121-132. DOI: 10.1016/j.foodchem.2017.05.097. View

12.

Nowosad K, Sujka M, Pankiewicz U, Kowalski R . The application of PEF technology in food processing and human nutrition. J Food Sci Technol. 2021; 58(2):397-411. PMC: 7847884. DOI: 10.1007/s13197-020-04512-4. View

13.

de la Fuente B, Pallares N, Berrada H, Barba F . Development of Antioxidant Protein Extracts from Gilthead Sea Bream () Side Streams Assisted by Pressurized Liquid Extraction (PLE). Mar Drugs. 2021; 19(4). PMC: 8066512. DOI: 10.3390/md19040199. View

14.

Melendez-Martinez A, Bohm V, Borge G, Cano M, Fikselova M, Gruskiene R . Carotenoids: Considerations for Their Use in Functional Foods, Nutraceuticals, Nutricosmetics, Supplements, Botanicals, and Novel Foods in the Context of Sustainability, Circular Economy, and Climate Change. Annu Rev Food Sci Technol. 2021; 12:433-460. DOI: 10.1146/annurev-food-062220-013218. View

15.

Devesa R, Moldes A, Diaz-Fierros F, Barral M . Extraction study of algal pigments in river bed sediments by applying factorial designs. Talanta. 2008; 72(4):1546-51. DOI: 10.1016/j.talanta.2007.02.006. View

16.

Zuluaga M, Barzegari A, Letourneur D, Gueguen V, Pavon-Djavid G . Oxidative Stress Regulation on Endothelial Cells by Hydrophilic Astaxanthin Complex: Chemical, Biological, and Molecular Antioxidant Activity Evaluation. Oxid Med Cell Longev. 2017; 2017:8073798. PMC: 5635468. DOI: 10.1155/2017/8073798. View

17.

Melendez-Martinez A, Mandic A, Bantis F, Bohm V, Borge G, Brncic M . A comprehensive review on carotenoids in foods and feeds: , applications, patents, and research needs. Crit Rev Food Sci Nutr. 2021; 62(8):1999-2049. DOI: 10.1080/10408398.2020.1867959. View

18.

Gan S, Liang S, Zou Q, Shang C . Optimization of carotenoid extraction of a halophilic microalgae. PLoS One. 2022; 17(8):e0270650. PMC: 9345481. DOI: 10.1371/journal.pone.0270650. View

19.

de la Fuente B, Pallares N, Barba F, Berrada H . An Integrated Approach for the Valorization of Sea Bass () Side Streams: Evaluation of Contaminants and Development of Antioxidant Protein Extracts by Pressurized Liquid Extraction. Foods. 2021; 10(3). PMC: 8000804. DOI: 10.3390/foods10030546. View

20.

Passi S, Cataudella S, Di Marco P, De Simone F, Rastrelli L . Fatty acid composition and antioxidant levels in muscle tissue of different Mediterranean marine species of fish and shellfish. J Agric Food Chem. 2002; 50(25):7314-22. DOI: 10.1021/jf020451y. View