Bioactive Synthetic Polymer-Based Polyelectrolyte LbL Coating Assembly on Surface Treated AZ31-Mg Alloys

Overview

Journal J Funct Biomater

Specialty Biomedical Engineering

Date 2023 Feb 24

PMID 36826874

Authors

Sangeetha Kunjukunju

Abhijit Roy

John Ohodnicki

Boeun Lee

Joe E Candiello

Mitali Patil

Prashant N Kumta

Affiliations

Soon will be listed here.

Abstract

Polyelectrolyte layer-by-layer (LbL) films on pretreated Mg containing 3 wt.% Al and 1 wt.% Zn (MgAZ31) alloy surfaces were prepared under physiological conditions offering improved bioresponse and corrosive protection. Pretreatments of the model MgAZ31 substrate surfaces were performed by alkaline and fluoride coating methods. The anti-corrosion and cytocompatibility behavior of pretreated substrates were evaluated. The LbL film assembly consisted of an initial layer of polyethyleneimine (PEI), followed by alternate layers of poly (lactic-co-glycolic acid) (PLGA) and poly (allylamine hydrochloride) (PAH), which self-arrange via electrostatic interactions on the pretreated MgAZ31 alloy substrate surface. The physicochemical characterization, surface morphologies, and microstructures of the LbL films were investigated using Fourier-transformed infrared spectroscopy (FTIR), atomic force microscopy (AFM), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The in vitro stability studies related to the LbL coatings confirmed that the surface treatments are imperative to achieve the lasting stability of PLGA/PAH layers. Electrochemical impedance spectroscopy measurements demonstrated that pretreated and LbL multilayered coated substrates enhanced the corrosion resistance of the bare MgAZ31 alloy. Cytocompatibility studies using human mesenchymal stem cells seeded directly over the substrates showed that the pretreated and LbL-generated surfaces were more cytocompatible, displaying reduced cytotoxicity than the bare MgAZ31. The release of bovine serum albumin protein from the LbL films was also studied. The initial data presented cooperatively demonstrate the promise of creating LbL layers on Mg-related bioresorbable scaffolds to obtain improved surface bio-related activity.

References

Tian J, Xu R, Wang H, Guan Y, Zhang Y . Precise and tunable time-controlled drug release system using layer-by-layer films as erodible coatings. Mater Sci Eng C Mater Biol Appl. 2020; 116:111244. DOI: 10.1016/j.msec.2020.111244. View

Larkin A, Davis R, Rajagopalan P . Biocompatible, detachable, and free-standing polyelectrolyte multilayer films. Biomacromolecules. 2010; 11(10):2788-96. DOI: 10.1021/bm100867h. View

Fan F, Zhou C, Wang X, Szpunar J . Layer-by-Layer Assembly of a Self-Healing Anticorrosion Coating on Magnesium Alloys. ACS Appl Mater Interfaces. 2015; 7(49):27271-8. DOI: 10.1021/acsami.5b08577. View

Gao F, Hu Y, Li G, Liu S, Quan L, Yang Z . Layer-by-layer deposition of bioactive layers on magnesium alloy stent materials to improve corrosion resistance and biocompatibility. Bioact Mater. 2020; 5(3):611-623. PMC: 7212186. DOI: 10.1016/j.bioactmat.2020.04.016. View

Pereda M, Alonso C, Burgos-Asperilla L, del Valle J, Ruano O, Perez P . Corrosion inhibition of powder metallurgy Mg by fluoride treatments. Acta Biomater. 2009; 6(5):1772-82. DOI: 10.1016/j.actbio.2009.11.004. View

Richert L, Boulmedais F, Lavalle P, Mutterer J, Ferreux E, Decher G . Improvement of stability and cell adhesion properties of polyelectrolyte multilayer films by chemical cross-linking. Biomacromolecules. 2004; 5(2):284-94. DOI: 10.1021/bm0342281. View

Jewell C, Lynn D . Multilayered polyelectrolyte assemblies as platforms for the delivery of DNA and other nucleic acid-based therapeutics. Adv Drug Deliv Rev. 2008; 60(9):979-99. PMC: 2476211. DOI: 10.1016/j.addr.2008.02.010. View

Zhao Y, Chen X, Li S, Zeng R, Zhang F, Wang Z . Corrosion resistance and drug release profile of gentamicin-loaded polyelectrolyte multilayers on magnesium alloys: Effects of heat treatment. J Colloid Interface Sci. 2019; 547:309-317. DOI: 10.1016/j.jcis.2019.04.017. View

Kim S, Kim Y, Kim K, Lee K, Lee M . Enhancement of bone formation on LBL-coated Mg alloy depending on the different concentration of BMP-2. Colloids Surf B Biointerfaces. 2018; 173:437-446. DOI: 10.1016/j.colsurfb.2018.09.061. View

10.

von Burkersroda F, Schedl L, Gopferich A . Why degradable polymers undergo surface erosion or bulk erosion. Biomaterials. 2002; 23(21):4221-31. DOI: 10.1016/s0142-9612(02)00170-9. View

11.

Hornberger H, Virtanen S, Boccaccini A . Biomedical coatings on magnesium alloys - a review. Acta Biomater. 2012; 8(7):2442-55. DOI: 10.1016/j.actbio.2012.04.012. View

12.

Schneider A, Vodouhe C, Richert L, Francius G, Le Guen E, Schaaf P . Multifunctional polyelectrolyte multilayer films: combining mechanical resistance, biodegradability, and bioactivity. Biomacromolecules. 2007; 8(1):139-45. PMC: 2535908. DOI: 10.1021/bm060765k. View

13.

Lu P, Fan H, Liu Y, Cao L, Wu X, Xu X . Controllable biodegradability, drug release behavior and hemocompatibility of PTX-eluting magnesium stents. Colloids Surf B Biointerfaces. 2010; 83(1):23-8. DOI: 10.1016/j.colsurfb.2010.10.038. View

14.

Kunjukunju S, Roy A, Ramanathan M, Lee B, Candiello J, Kumta P . A layer-by-layer approach to natural polymer-derived bioactive coatings on magnesium alloys. Acta Biomater. 2013; 9(10):8690-703. DOI: 10.1016/j.actbio.2013.05.013. View

15.

Ostrowski N, Lee B, Enick N, Carlson B, Kunjukunju S, Roy A . Corrosion protection and improved cytocompatibility of biodegradable polymeric layer-by-layer coatings on AZ31 magnesium alloys. Acta Biomater. 2013; 9(10):8704-13. DOI: 10.1016/j.actbio.2013.05.010. View

16.

Witte F . The history of biodegradable magnesium implants: a review. Acta Biomater. 2010; 6(5):1680-92. DOI: 10.1016/j.actbio.2010.02.028. View

17.

Janning C, Willbold E, Vogt C, Nellesen J, Meyer-Lindenberg A, Windhagen H . Magnesium hydroxide temporarily enhancing osteoblast activity and decreasing the osteoclast number in peri-implant bone remodelling. Acta Biomater. 2009; 6(5):1861-8. DOI: 10.1016/j.actbio.2009.12.037. View

18.

Wang J, Tang J, Zhang P, Li Y, Wang J, Lai Y . Surface modification of magnesium alloys developed for bioabsorbable orthopedic implants: a general review. J Biomed Mater Res B Appl Biomater. 2012; 100(6):1691-701. DOI: 10.1002/jbm.b.32707. View

19.

Tian P, Liu X . Surface modification of biodegradable magnesium and its alloys for biomedical applications. Regen Biomater. 2016; 2(2):135-51. PMC: 4669019. DOI: 10.1093/rb/rbu013. View

20.

Mendelsohn J, Yang S, Hiller J, Hochbaum A, Rubner M . Rational design of cytophilic and cytophobic polyelectrolyte multilayer thin films. Biomacromolecules. 2003; 4(1):96-106. DOI: 10.1021/bm0256101. View