Long-read Assemblies Reveal Structural Diversity in Genomes of Organelles - an Example with

Overview

Journal GigaByte

Date 2023 Feb 24

PMID 36824345

Authors

Anna E Syme

Todd G B McLay

Frank Udovicic

David J Cantrill

Daniel J Murphy

Affiliations

Soon will be listed here.

Abstract

Organelle genomes are typically represented as single, static, circular molecules. However, there is evidence that the chloroplast genome exists in two structural haplotypes and that the mitochondrial genome can display multiple circular, linear or branching forms. We sequenced and assembled chloroplast and mitochondrial genomes of the Golden Wattle, , using long reads, iterative baiting to extract organelle-only reads, and several assembly algorithms to explore genomic structure. Using a assembly approach agnostic to previous hypotheses about structure, we found that different assemblies revealed contrasting arrangements of genomic segments; a hypothesis supported by mapped reads spanning alternate paths.

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