Differential Proteomic Patterns of Plasma Extracellular Vesicles Show Potential to Discriminate β-thalassemia Subtypes

Overview

Journal iScience

Publisher Cell Press

Date 2023 Feb 24

PMID 36824279

Authors

Na Li

Bowen Wu

Jifeng Wang

Yumeng Yan

Peng An

Yuezhen Li

Yuning Liu

Yanfei Hou

Xiaoqing Qing

Lili Niu

Xiang Ding

Zhensheng Xie

Mengmeng Zhang

Xiaojing Guo

Xiulan Chen

Tanxi Cai

Jianming Luo

Fudi Wang

Fuquan Yang

Affiliations

Soon will be listed here.

Abstract

The observed specificity of β-thalassemia-subtype phenotypes makes new diagnostic strategies that complement current screening methods necessary to determine each subtype and facilitate therapeutic regimens for different patients. Here, we performed quantitative proteomics of plasma-derived extracellular vesicles (EVs) of β-thalassemia major (TM) patients, β-thalassemia intermedia (TI) patients, and healthy controls to explore subgroup characteristics and potential biomarkers. Plasma quantitative proteomics among the same cohorts were analyzed in parallel to compare the biomarker potential of both specimens. EV proteomics showed significantly more abnormalities in immunity and lipid metabolism in TI and TM, respectively. The differential proteomic patterns of EVs were consistent with but more striking than those of plasma. Notably, we also found EV proteins to have a superior performance for discriminating β-thalassemia subtypes. These findings allowed us to propose a diagnostic model consisting of five proteins in EVs with subtyping potential, demonstrating the ability of plasma-derived EVs for the diagnosis of β-thalassemia patients.

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