Immobilization of Bacterial Mixture of FH-1 and Sp. NJ-1 Enhances the Bioremediation of Atrazine-polluted Soil Environments

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Feb 23

PMID 36819060

Authors

Zequn Pan

Yulin Wu

Qianhang Zhai

Yanan Tang

Xuewei Liu

Xuanwei Xu

Shuang Liang

Hao Zhang

Affiliations

Soon will be listed here.

Abstract

In this study, the effects of the immobilized bacterial mixture (IM-FN) of sp. NJ-1 and strain FH-1 using sodium alginate-CaCl on the degradation of atrazine were investigated. The results showed that the optimal ratio of three types of carrier materials (i.e., rice straw powder, rice husk, and wheat bran) was 1:1:1 with the highest adsorption capacity for atrazine (i.e., 3774.47 mg/kg) obtained at 30°C. On day 9, the degradation efficiency of atrazine (50 mg/L) reached 98.23% with cell concentration of 1.6 × 10 cfu/ml at pH 9 and 30°C. The Box-Behnken method was used to further optimize the culture conditions for the degradation of atrazine by the immobilized bacterial mixture. The IM-FN could be reused for 2-3 times with the degradation efficiency of atrazine maintained at 73.0% after being stored for 80 days at 25°C. The population dynamics of IM-FN was explored with the total soil DNA samples specifically analyzed by real-time PCR. In 7 days, the copy numbers of both and genes in the IM-FN were significantly higher than those of bacterial suspensions in the soil. Compared with bacterial suspensions, the IM-FN significantly accelerated the degradation of atrazine (20 mg/kg) in soil with the half-life shortened from 19.80 to 7.96 days. The plant heights of two atrazine-sensitive crops (wheat and soybean) were increased by 14.99 and 64.74%, respectively, in the soil restored by immobilized bacterial mixture, indicating that the IM-FN significantly reduced the phytotoxicity of atrazine on the plants. Our study evidently demonstrated that the IM-FN could significantly increase the degradation of atrazine, providing a potentially effective bioremediation technique for the treatment of atrazine-polluted soil environment and providing experimental support for the wide application of immobilized microorganism technology in agriculture.

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References

Duran-Bedolla J, Garza-Ramos U, Rodriguez-Medina N, Aguilar Vera A, Barrios-Camacho H . Exploring the environmental traits and applications of Klebsiella variicola. Braz J Microbiol. 2021; 52(4):2233-2245. PMC: 8578232. DOI: 10.1007/s42770-021-00630-z. View

Zhang J, Lu Y, Zhang J, Tan L, Yang H . Accumulation and toxicological response of atrazine in rice crops. Ecotoxicol Environ Saf. 2014; 102:105-12. DOI: 10.1016/j.ecoenv.2013.12.034. View

Lin Z, Zhen Z, Liang Y, Li J, Yang J, Zhong L . Changes in atrazine speciation and the degradation pathway in red soil during the vermiremediation process. J Hazard Mater. 2018; 364:710-719. DOI: 10.1016/j.jhazmat.2018.04.037. View

Ali N, Jabbar N, Alardhi S, Majdi H, Albayati T . Adsorption of methyl violet dye onto a prepared bio-adsorbent from date seeds: isotherm, kinetics, and thermodynamic studies. Heliyon. 2022; 8(8):e10276. PMC: 9420514. DOI: 10.1016/j.heliyon.2022.e10276. View

Ma L, Zhang N, Liu J, Yan Zhai X, Lv Y, Lu F . Uptake of atrazine in a paddy crop activates an epigenetic mechanism for degrading the pesticide in plants and environment. Environ Int. 2019; 131:105014. DOI: 10.1016/j.envint.2019.105014. View

Wolf D, Cryder Z, Gan J . Soil bacterial community dynamics following surfactant addition and bioaugmentation in pyrene-contaminated soils. Chemosphere. 2019; 231:93-102. DOI: 10.1016/j.chemosphere.2019.05.145. View

Miller J, Techtmann S, Fortney J, Mahmoudi N, Joyner D, Liu J . Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities. Front Microbiol. 2019; 10:995. PMC: 6521576. DOI: 10.3389/fmicb.2019.00995. View

Zhao X, Wang L, Ma F, Bai S, Yang J, Qi S . Pseudomonas sp. ZXY-1, a newly isolated and highly efficient atrazine-degrading bacterium, and optimization of biodegradation using response surface methodology. J Environ Sci (China). 2017; 54:152-159. DOI: 10.1016/j.jes.2016.06.010. View

Ren W, Liu H, Mao T, Teng Y, Zhao R, Luo Y . Enhanced remediation of PAHs-contaminated site soil by bioaugmentation with graphene oxide immobilized bacterial pellets. J Hazard Mater. 2022; 433:128793. DOI: 10.1016/j.jhazmat.2022.128793. View

10.

Luo S, Ren L, Wu W, Chen Y, Li G, Zhang W . Impacts of earthworm casts on atrazine catabolism and bacterial community structure in laterite soil. J Hazard Mater. 2021; 425:127778. DOI: 10.1016/j.jhazmat.2021.127778. View

11.

Guo Z, Ouyang W, Xavier Supe T, Lin C, He M, Wang B . Gradient of suspended particulate matter hastens the multi-interface partition dynamics of atrazine and its degradation products. Environ Pollut. 2022; 315:120432. DOI: 10.1016/j.envpol.2022.120432. View

12.

Zhu S, Zhang T, Wang Y, Zhou X, Wang S, Wang Z . Meta-analysis and experimental validation identified atrazine as a toxicant in the male reproductive system. Environ Sci Pollut Res Int. 2021; 28(28):37482-37497. DOI: 10.1007/s11356-021-13396-6. View

13.

Wang J, Zhu L, Liu A, Ma T, Wang Q, Xie H . Isolation and characterization of an Arthrobacter sp. strain HB-5 that transforms atrazine. Environ Geochem Health. 2010; 33(3):259-66. DOI: 10.1007/s10653-010-9337-3. View

14.

Yu J, He H, Yang W, Yang C, Zeng G, Wu X . Magnetic bionanoparticles of Penicillium sp. yz11-22N2 doped with FeO and encapsulated within PVA-SA gel beads for atrazine removal. Bioresour Technol. 2018; 260:196-203. DOI: 10.1016/j.biortech.2018.03.103. View

15.

Getenga Z, Dorfler U, Iwobi A, Schmid M, Schroll R . Atrazine and terbuthylazine mineralization by an Arthrobacter sp. isolated from a sugarcane-cultivated soil in Kenya. Chemosphere. 2009; 77(4):534-9. DOI: 10.1016/j.chemosphere.2009.07.031. View

16.

Liu Y, Gan L, Chen Z, Megharaj M, Naidu R . Removal of nitrate using Paracoccus sp. YF1 immobilized on bamboo carbon. J Hazard Mater. 2012; 229-230:419-25. DOI: 10.1016/j.jhazmat.2012.06.029. View

17.

Zhang B, Ni Y, Liu J, Yan T, Zhu X, Li Q . Bead-immobilized Pseudomonas stutzeri Y2 prolongs functions to degrade s-triazine herbicides in industrial wastewater and maize fields. Sci Total Environ. 2020; 731:139183. DOI: 10.1016/j.scitotenv.2020.139183. View

18.

Hansen S, Messer T, Mittelstet A . Mitigating the risk of atrazine exposure: Identifying hot spots and hot times in surface waters across Nebraska, USA. J Environ Manage. 2019; 250:109424. DOI: 10.1016/j.jenvman.2019.109424. View

19.

Zaya R, Amini Z, Whitaker A, Kohler S, Ide C . Atrazine exposure affects growth, body condition and liver health in Xenopus laevis tadpoles. Aquat Toxicol. 2011; 104(3-4):243-53. DOI: 10.1016/j.aquatox.2011.04.021. View

20.

Pan X, Wang S, Shi N, Fang H, Yu Y . Biodegradation and detoxification of chlorimuron-ethyl by Enterobacter ludwigii sp. CE-1. Ecotoxicol Environ Saf. 2017; 150:34-39. DOI: 10.1016/j.ecoenv.2017.12.023. View