Heterologous Expression of Recombinant Human Cytochrome P450 (CYP) in : N-Terminal Modification, Expression, Isolation, Purification, and Reconstitution

Overview

Journal BioTech (Basel)

Specialty Biotechnology

Date 2023 Feb 22

PMID 36810444

Authors

Tao Shang

Chee Mun Fang

Chin Eng Ong

Yan Pan

Affiliations

Soon will be listed here.

Abstract

Cytochrome P450 (CYP) enzymes play important roles in metabolising endogenous and xenobiotic substances. Characterisations of human CYP proteins have been advanced with the rapid development of molecular technology that allows heterologous expression of human CYPs. Among several hosts, bacteria systems such as () have been widely used thanks to their ease of use, high level of protein yields, and affordable maintenance costs. However, the levels of expression in reported in the literature sometimes differ significantly. This paper aims to review several contributing factors, including N-terminal modifications, co-expression with a chaperon, selections of vectors and strains, bacteria culture and protein expression conditions, bacteria membrane preparations, CYP protein solubilizations, CYP protein purifications, and reconstitution of CYP catalytic systems. The common factors that would most likely lead to high expression of CYPs were identified and summarised. Nevertheless, each factor may still require careful evaluation for individual CYP isoforms to achieve a maximal expression level and catalytic activity. Recombinant systems have been evidenced as a useful tool in obtaining the ideal level of human CYP proteins, which ultimately allows for subsequent characterisations of structures and functions.

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