Chondromodulin is Necessary for Cartilage Callus Distraction in Mice

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2023 Feb 16

PMID 36795722

Authors

Kiminori Yukata

Chisa Shukunami

Yoshito Matsui

Aki Takimoto

Tomohiro Goto

Mitsuhiko Takahashi

Atsushi Mihara

Tetsuya Seto

Takashi Sakai

Yuji Hiraki

Natsuo Yasui

Affiliations

Soon will be listed here.

Abstract

Chondromodulin (Cnmd) is a glycoprotein known to stimulate chondrocyte growth. We examined in this study the expression and functional role of Cnmd during distraction osteogenesis that is modulated by mechanical forces. The right tibiae of the mice were separated by osteotomy and subjected to slow progressive distraction using an external fixator. In situ hybridization and immunohistochemical analyses of the lengthened segment revealed that Cnmd mRNA and its protein in wild-type mice were localized in the cartilage callus, which was initially generated in the lag phase and was lengthened gradually during the distraction phase. In Cnmd null (Cnmd-/-) mice, less cartilage callus was observed, and the distraction gap was filled by fibrous tissues. Additionally, radiological and histological investigations demonstrated delayed bone consolidation and remodeling of the lengthened segment in Cnmd-/- mice. Eventually, Cnmd deficiency caused a one-week delay in the peak expression of VEGF, MMP2, and MMP9 genes and the subsequent angiogenesis and osteoclastogenesis. We conclude that Cnmd is necessary for cartilage callus distraction.

Citing Articles

A Narrative Review of the Roles of Chondromodulin-I (Cnmd) in Adult Cartilage Tissue.

Reyes Alcaraz V, Pattappa G, Miura S, Angele P, Blunk T, Rudert M Int J Mol Sci. 2024; 25(11).

PMID: 38892027 PMC: 11173128. DOI: 10.3390/ijms25115839.

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