Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis

Overview

Journal Int J Dent

Publisher Wiley

Specialty Dentistry

Date 2023 Feb 16

PMID 36794024

Authors

Fumie Shiba

Hisako Furusho

Takashi Takata

Rika Shimizu

Mutsumi Miyauchi

Affiliations

Soon will be listed here.

Abstract

Background And Aims: extract (EA) exerts various biological effects, including anti-inflammatory activity. The effect of EA on alveolar bone destruction has not been reported; therefore, we aimed to determine whether EA could inhibit alveolar bone destruction associated with periodontitis in a rat model in which periodontitis was induced using lipopolysaccharide from (. -LPS).

Methods: Physiological saline or . -LPS or . -LPS/EA mixture was topically administered into the gingival sulcus of the upper molar region of the rats. After 3 days, periodontal tissues of the molar region were collected. Immunohistochemistry was performed for cathepsin K, receptor activator of NF-B ligand (RANKL), and osteoprotegerin (OPG). The cathepsin K-positive osteoclasts along the alveolar bone margin were counted. EA effects on the expression of the factors regulating osteoclastogenesis in osteoblasts with . -LPS-stimulation were also examined .

Results: Treatment with EA significantly reduced the number of osteoclasts by decreasing the RANKL-expression and increasing OPG-expression in the periodontal ligament in the treatment group compared to the . -LPS group. The study showed that the upregulation of p-IB kinase and (p-IKK/), p-NF-B p65, TNF-, interleukin-6, and RANKL and downregulation of semaphorin 3A (Sema3A), -catenin, and OPG in the osteoblasts with . -LPS-stimulation improved with EA-treatment.

Conclusion: These findings demonstrated that topical EA suppressed alveolar bone resorption in the rat model with . -LPS-induced periodontitis by maintaining a balance in RANKL/OPG ratio via the pathways of NF-B, Wnt/-catenin, and Sema3A/Neuropilin-1. Therefore, EA possesses the potential to prevent bone destruction through inhibiting osteoclastogenesis attributed to cytokine burst under plaque accumulation.

Citing Articles

Semaphorin 3A on Osteoporosis: An Overreview of the Literature.

Zhang Y, Shi H, Dai X, Shen J, Yin J, Xu T Calcif Tissue Int. 2025; 116(1):43.

PMID: 39985619 DOI: 10.1007/s00223-025-01350-4.

Antinociceptive effect of Equisetum arvense extract on the stomatitis hamster model.

Shiba F, Maekawara S, Inoue A, Ohta K, Miyauchi M PLoS One. 2024; 19(11):e0313747.

PMID: 39570913 PMC: 11581248. DOI: 10.1371/journal.pone.0313747.

Phytochemical Investigation of and Evaluation of Their Anti-Inflammatory Potential in TNFα/INFγ-Stimulated Keratinocytes.

Jeong S, Yu H, Ra M, Jung S, Yu J, Kim J Pharmaceuticals (Basel). 2023; 16(10).

PMID: 37895949 PMC: 10609812. DOI: 10.3390/ph16101478.

References

Littlewood-Evans A, Kokubo T, Ishibashi O, Inaoka T, Wlodarski B, Gallagher J . Localization of cathepsin K in human osteoclasts by in situ hybridization and immunohistochemistry. Bone. 1997; 20(2):81-6. DOI: 10.1016/s8756-3282(96)00351-1. View

Dai J, Peng L, Fan K, Wang H, Wei R, Ji G . Osteopontin induces angiogenesis through activation of PI3K/AKT and ERK1/2 in endothelial cells. Oncogene. 2009; 28(38):3412-22. DOI: 10.1038/onc.2009.189. View

Tanabe N, Maeno M, Suzuki N, Fujisaki K, Tanaka H, Ogiso B . IL-1 alpha stimulates the formation of osteoclast-like cells by increasing M-CSF and PGE2 production and decreasing OPG production by osteoblasts. Life Sci. 2005; 77(6):615-26. DOI: 10.1016/j.lfs.2004.10.079. View

Wang H, Qin H, Deng H, Sun C, Hu R . [Effects of LPS-stimulated monocyte culture supernatant on the OPG/RANKL of osteoblastic cells]. Shanghai Kou Qiang Yi Xue. 2015; 24(4):428-32. View

Milovanovic V, Radulovic N, Todorovic Z, Stankovic M, Stojanovic G . Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species. Plant Foods Hum Nutr. 2007; 62(3):113-9. DOI: 10.1007/s11130-007-0050-z. View

Chuang F, Tsai C, Chen J, Chen K, Chen Y, Lin Y . Long-term sequential receptor activator of NF-κB ligand (RANKL) and osteoprotegrin (OPG) expression in lipopolysaccharide-induced rat periapical lesions. J Oral Pathol Med. 2011; 41(2):186-93. DOI: 10.1111/j.1600-0714.2011.01065.x. View

Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H . Osteoprotection by semaphorin 3A. Nature. 2012; 485(7396):69-74. DOI: 10.1038/nature11000. View

Pang J, Ricupero D, Huang S, Fatma N, Singh D, Romero J . Differential activity of kaempferol and quercetin in attenuating tumor necrosis factor receptor family signaling in bone cells. Biochem Pharmacol. 2006; 71(6):818-26. DOI: 10.1016/j.bcp.2005.12.023. View

Anandarajah A, Schwarz E, Totterman S, Monu J, Feng C, Shao T . The effect of etanercept on osteoclast precursor frequency and enhancing bone marrow oedema in patients with psoriatic arthritis. Ann Rheum Dis. 2007; 67(3):296-301. DOI: 10.1136/ard.2007.076091. View

10.

Shiba F, Miyauchi M, Chea C, Furusho H, Iwasaki S, Shimizu R . Anti-inflammatory effect of glycyrrhizin with Equisetum arvense extract. Odontology. 2020; 109(2):464-473. DOI: 10.1007/s10266-020-00563-3. View

11.

Bian Q, Liu S, Huang J, Yang Z, Tang D, Zhou Q . Oleanolic acid exerts an osteoprotective effect in ovariectomy-induced osteoporotic rats and stimulates the osteoblastic differentiation of bone mesenchymal stem cells in vitro. Menopause. 2011; 19(2):225-33. DOI: 10.1097/gme.0b013e3182272ef1. View

12.

Mosca L, Grady D, Barrett-Connor E, Collins P, Wenger N, Abramson B . Effect of raloxifene on stroke and venous thromboembolism according to subgroups in postmenopausal women at increased risk of coronary heart disease. Stroke. 2008; 40(1):147-55. PMC: 3559135. DOI: 10.1161/STROKEAHA.108.518621. View

13.

Yu X, Lv L, Zhang J, Zhang T, Xiao C, Li S . Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats. J Mol Histol. 2015; 46(2):195-203. DOI: 10.1007/s10735-015-9611-x. View

14.

Kling J, Clarke B, Sandhu N . Osteoporosis prevention, screening, and treatment: a review. J Womens Health (Larchmt). 2014; 23(7):563-72. PMC: 4089021. DOI: 10.1089/jwh.2013.4611. View

15.

Heitz-Mayfield L, Lang N . Surgical and nonsurgical periodontal therapy. Learned and unlearned concepts. Periodontol 2000. 2013; 62(1):218-31. DOI: 10.1111/prd.12008. View

16.

Khan A, Morrison A, Hanley D, Felsenberg D, McCauley L, ORyan F . Diagnosis and management of osteonecrosis of the jaw: a systematic review and international consensus. J Bone Miner Res. 2014; 30(1):3-23. DOI: 10.1002/jbmr.2405. View

17.

Oka H, Miyauchi M, Furusho H, Nishihara T, Takata T . Oral administration of prostaglandin E(2)-specific receptor 4 antagonist inhibits lipopolysaccharide-induced osteoclastogenesis in rat periodontal tissue. J Periodontol. 2011; 83(4):506-13. DOI: 10.1902/jop.2011.110301. View

18.

Guo C, Hou G, Li X, Xia X, Liu D, Huang D . Quercetin triggers apoptosis of lipopolysaccharide (LPS)-induced osteoclasts and inhibits bone resorption in RAW264.7 cells. Cell Physiol Biochem. 2012; 30(1):123-36. DOI: 10.1159/000339052. View

19.

Grundemann C, Lengen K, Sauer B, Garcia-Kaufer M, Zehl M, Huber R . Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells. BMC Complement Altern Med. 2014; 14:283. PMC: 4132922. DOI: 10.1186/1472-6882-14-283. View

20.

Zhang Q, Guo R, Schwarz E, Boyce B, Xing L . TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood. Arthritis Res Ther. 2008; 10(2):R37. PMC: 2453755. DOI: 10.1186/ar2391. View