Removal of the Endoplasma Membrane Upon Sperm Cell Activation After Pollen Tube Discharge

Overview

Journal Front Plant Sci

Date 2023 Feb 13

PMID 36778680

Authors

Naoya Sugi

Rie Izumi

Shun Tomomi

Daichi Susaki

Tetsu Kinoshita

Daisuke Maruyama

Affiliations

Soon will be listed here.

Abstract

In pollen and pollen tubes, immotile sperm cells are enclosed by an inner vegetative plasma membrane (IVPM), a single endomembrane originating from the vegetative-cell plasma membrane. It is widely believed that sperm cells must be removed from the IVPM prior to gamete associations and fusions; however, details of the timing and morphological changes upon IVPM dissociation remain elusive. Here, we report a rapid IVPM breakdown immediately before double fertilization in . The IVPM was stably observed in coiling pollen tubes when pollen tube discharge was prevented using mutant ovules. In contrast, a semi- fertilization assay in wild-type ovules demonstrated fragmented IVPM around sperm nuclei 1 min after pollen tube discharge. These observations revealed the dynamic alteration of released sperm cells and provided new insights into double fertilization in flowering plants. With a summary of recent findings on IVPM lipid composition, we discussed the possible physiological signals controlling IVPM breakdown.

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