MiR-141-3p Targeted SIRT1 to Inhibit Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2023 Feb 13

PMID 36777717

Authors

Shuzuo Zhou

Gang Zhang

Kun Wang

Zhong Yang

Yinghui Tan

Affiliations

Soon will be listed here.

Abstract

Purpose: To explore the expression of miR-141-3p during the osteogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs) and its regulatory effect.

Methods: Differentiation of BMSCs was induced by dexamethasone. The mRNA expression of miR-141-3p, ALP, RUNX2, and OCN was measured using RT-qPCR. The protein expression was detected via western blot. The target of miR-141-3p was predicted through the TargetScan website and confirmed using luciferase reporter assay.

Results: miR-141-3p expression declined during osteogenic differentiation. The relative ALP activities and the mRNA expression of ALP, RUNX2, and OCN were markedly reduced in the miR-141-3p mimic group while increased in the inhibitor group. Cell viability was suppressed in the miR-141-3p mimic group and promoted in the inhibitor group. SIRT1 was predicted to be a downstream gene of miR-141-3p, and this prediction was confirmed via the luciferase reporter assay. The results of the western blot assay demonstrated that SIRT1 expression was decreased in the miR-141-3p mimic group. SIRT1 reversed the inhibitory influence of miR-141-3p on the osteogenic differentiation ability of BMSCs.

Conclusion: miR-141-3p targeted SIRT1 to inhibit osteogenic differentiation of BMSCs via the Wnt/-catenin signaling pathway.

Citing Articles

Retracted: miR-141-3p Targeted SIRT1 to Inhibit Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells.

International S Stem Cells Int. 2024; 2024:9870790.

PMID: 38298236 PMC: 10830318. DOI: 10.1155/2024/9870790.

References

Liu G, David B, Trawczynski M, Fessler R . Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications. Stem Cell Rev Rep. 2019; 16(1):3-32. PMC: 6987053. DOI: 10.1007/s12015-019-09935-x. View

Wu F, Huang W, Yang Y, Liu F, Chen J, Wang G . miR-155-5p regulates mesenchymal stem cell osteogenesis and proliferation by targeting GSK3B in steroid-associated osteonecrosis. Cell Biol Int. 2020; 45(1):83-91. DOI: 10.1002/cbin.11470. View

Abed E, Couchourel D, Delalandre A, Duval N, Pelletier J, Martel-Pelletier J . Low sirtuin 1 levels in human osteoarthritis subchondral osteoblasts lead to abnormal sclerostin expression which decreases Wnt/β-catenin activity. Bone. 2013; 59:28-36. DOI: 10.1016/j.bone.2013.10.020. View

Long H, Sun B, Cheng L, Zhao S, Zhu Y, Zhao R . miR-139-5p Represses BMSC Osteogenesis via Targeting Wnt/β-Catenin Signaling Pathway. DNA Cell Biol. 2017; 36(8):715-724. DOI: 10.1089/dna.2017.3657. View

Zhang N, Lyu Y, Pan X, Xu L, Xuan A, He X . miR‑146b‑5p promotes the neural conversion of pluripotent stem cells by targeting Smad4. Int J Mol Med. 2017; 40(3):814-824. PMC: 5548013. DOI: 10.3892/ijmm.2017.3064. View

Kang I, Jeong B, Hur S, Choi H, Choi S, Ryu J . MicroRNA-302a stimulates osteoblastic differentiation by repressing COUP-TFII expression. J Cell Physiol. 2014; 230(4):911-21. DOI: 10.1002/jcp.24822. View

Anderson B, McAlinden A . miR-483 targets SMAD4 to suppress chondrogenic differentiation of human mesenchymal stem cells. J Orthop Res. 2017; 35(11):2369-2377. PMC: 5573664. DOI: 10.1002/jor.23552. View

Almeida M, Porter R . Sirtuins and FoxOs in osteoporosis and osteoarthritis. Bone. 2019; 121:284-292. PMC: 6812652. DOI: 10.1016/j.bone.2019.01.018. View

Lee H, Kim Y, Nirmala F, Kim J, Seo H, Ha T . MiR-141-3p promotes mitochondrial dysfunction in ovariectomy-induced sarcopenia via targeting Fkbp5 and Fibin. Aging (Albany NY). 2021; 13(4):4881-4894. PMC: 7950230. DOI: 10.18632/aging.202617. View

10.

Liang Z, Li X, Liu S, Li C, Wang X, Xing J . MiR-141-3p inhibits cell proliferation, migration and invasion by targeting TRAF5 in colorectal cancer. Biochem Biophys Res Commun. 2019; 514(3):699-705. DOI: 10.1016/j.bbrc.2019.05.002. View

11.

Pino A, Rosen C, Rodriguez J . In osteoporosis, differentiation of mesenchymal stem cells (MSCs) improves bone marrow adipogenesis. Biol Res. 2013; 45(3):279-87. PMC: 8262098. DOI: 10.4067/S0716-97602012000300009. View

12.

Lin Z, He H, Wang M, Liang J . MicroRNA-130a controls bone marrow mesenchymal stem cell differentiation towards the osteoblastic and adipogenic fate. Cell Prolif. 2019; 52(6):e12688. PMC: 6869834. DOI: 10.1111/cpr.12688. View

13.

Li D, Yuan Q, Xiong L, Li A, Xia Y . The miR-4739/DLX3 Axis Modulates Bone Marrow-Derived Mesenchymal Stem Cell (BMSC) Osteogenesis Affecting Osteoporosis Progression. Front Endocrinol (Lausanne). 2021; 12:703167. PMC: 8678599. DOI: 10.3389/fendo.2021.703167. View

14.

Zhao P, Xiao L, Peng J, Qian Y, Huang C . Exosomes derived from bone marrow mesenchymal stem cells improve osteoporosis through promoting osteoblast proliferation via MAPK pathway. Eur Rev Med Pharmacol Sci. 2018; 22(12):3962-3970. DOI: 10.26355/eurrev_201806_15280. View

15.

Feng G, Zheng K, Song D, Xu K, Huang D, Zhang Y . SIRT1 was involved in TNF-α-promoted osteogenic differentiation of human DPSCs through Wnt/β-catenin signal. In Vitro Cell Dev Biol Anim. 2016; 52(10):1001-1011. DOI: 10.1007/s11626-016-0070-9. View

16.

Kinane D, Stathopoulou P, Papapanou P . Periodontal diseases. Nat Rev Dis Primers. 2017; 3:17038. DOI: 10.1038/nrdp.2017.38. View

17.

Lee K, Kim H, Li Q, Chi X, Ueta C, Komori T . Runx2 is a common target of transforming growth factor beta1 and bone morphogenetic protein 2, and cooperation between Runx2 and Smad5 induces osteoblast-specific gene expression in the pluripotent mesenchymal precursor cell line C2C12. Mol Cell Biol. 2000; 20(23):8783-92. PMC: 86511. DOI: 10.1128/MCB.20.23.8783-8792.2000. View

18.

Scannapieco F, Gershovich E . The prevention of periodontal disease-An overview. Periodontol 2000. 2020; 84(1):9-13. DOI: 10.1111/prd.12330. View

19.

Zhang X, Wang Y, Zhao H, Han X, Zhao T, Qu P . Extracellular vesicle-encapsulated miR-22-3p from bone marrow mesenchymal stem cell promotes osteogenic differentiation via FTO inhibition. Stem Cell Res Ther. 2020; 11(1):227. PMC: 7285613. DOI: 10.1186/s13287-020-01707-6. View

20.

Shen Z, Kuang S, Zhang Y, Yang M, Qin W, Shi X . Chitosan hydrogel incorporated with dental pulp stem cell-derived exosomes alleviates periodontitis in mice via a macrophage-dependent mechanism. Bioact Mater. 2020; 5(4):1113-1126. PMC: 7371600. DOI: 10.1016/j.bioactmat.2020.07.002. View