Identification of MiRNAs Involving Potato- Interaction

Overview

Journal Plants (Basel)

Date 2023 Feb 11

PMID 36771544

Authors

Ming Luo

Xinyuan Sun

Meng Xu

Zhendong Tian

Affiliations

Soon will be listed here.

Abstract

sRNAs (small RNAs) play an important role in regulation of plant immunity against a variety of pathogens. In this study, sRNA sequencing analysis was performed to identify miRNAs (microRNAs) during the interaction of potato and . Totally, 171 potato miRNAs were identified, 43 of which were annotated in the miRNA database and 128 were assigned as novel miRNAs in this study. Those potato miRNAs may target 878 potato genes and half of them encode resistance proteins. Fifty-three potato miRNAs may target 194 genes. Three potato miRNAs (novel 72, 133, and 140) were predicted to have targets only in the genome. miRNAs transient expression and inoculation assay showed that miR396, miR166, miR6149-5P, novel133, or novel140 promoted colonization, while miR394 inhibited colonization on leaves. An artificial miRNA target (amiRNA) degradation experiment demonstrated that miR394 could target both potato gene () and genes. miR396 targets the multicystatin gene () and miR6149-5p could shear the galactose oxidase F-box protein gene (). This study provides new information on the aspect of cross-kingdom immune regulation in potato- interaction at the sRNAs regulation level.

Citing Articles

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