Comparative Analysis of the Response to Polyethylene Glycol-Simulated Drought Stress in Roots from Seedlings of "Modern" and "Ancient" Wheat Varieties

Overview

Journal Plants (Basel)

Date 2023 Feb 11

PMID 36771510

Authors

Ilva Licaj

Maria Chiara Di Meo

Anna Fiorillo

Simone Samperna

Mauro Marra

Mariapina Rocco

Affiliations

Soon will be listed here.

Abstract

Durum wheat is widely cultivated in the Mediterranean, where it is the basis for the production of high added-value food derivatives such as pasta. In the next few years, the detrimental effects of global climate change will represent a serious challenge to crop yields. For durum wheat, the threat of climate change is worsened by the fact that cultivation relies on a few genetically uniform, elite varieties, better suited to intensive cultivation than "traditional" ones but less resistant to environmental stress. Hence, the renewed interest in "ancient" traditional varieties are expected to be more tolerant to environmental stress as a source of genetic resources to be exploited for the selection of useful agronomic traits such as drought tolerance. The aim of this study was to perform a comparative analysis of the effect and response of roots from the seedlings of two durum wheat cultivars: Svevo, a widely cultivated elite variety, and Saragolla, a traditional variety appreciated for its organoleptic characteristics, to Polyethylene glycol-simulated drought stress. The effect of water stress on root growth was analyzed and related to biochemical data such as hydrogen peroxide production, electrolyte leakage, membrane lipid peroxidation, proline synthesis, as well as to molecular data such as qRT-PCR analysis of drought responsive genes and proteomic analysis of changes in the protein repertoire of roots from the two cultivars.

Citing Articles

Effect of Polyethylene Glycol-Simulated Drought Stress on Stomatal Opening in "Modern" and "Ancient" Wheat Varieties.

Licaj I, Fiorillo A, Di Meo M, Varricchio E, Rocco M Plants (Basel). 2024; 13(11).

PMID: 38891383 PMC: 11174684. DOI: 10.3390/plants13111575.

Genetic diversity of durum wheat (Triticum turgidum ssp. durum) to mitigate abiotic stress: Drought, heat, and their combination.

Chaouachi L, Marin-Sanz M, Barro F, Karmous C PLoS One. 2024; 19(4):e0301018.

PMID: 38574054 PMC: 10994418. DOI: 10.1371/journal.pone.0301018.

An artificial intelligence-integrated analysis of the effect of drought stress on root traits of "modern" and "ancient" wheat varieties.

Licaj I, Felice D, Germinario C, Zanotti C, Fiorillo A, Marra M Front Plant Sci. 2023; 14:1241281.

PMID: 37900753 PMC: 10613089. DOI: 10.3389/fpls.2023.1241281.

References

Mori I, Schroeder J . Reactive oxygen species activation of plant Ca2+ channels. A signaling mechanism in polar growth, hormone transduction, stress signaling, and hypothetically mechanotransduction. Plant Physiol. 2004; 135(2):702-8. PMC: 514107. DOI: 10.1104/pp.104.042069. View

Yuan Y, Song T, Yu J, Zhang W, Hou X, Kong Ling Z . Genome-Wide Investigation of the Cysteine Synthase Gene Family Shows That Overexpression of Confers Alkali Tolerance to Alfalfa ( L.). Front Plant Sci. 2022; 12:792862. PMC: 8765340. DOI: 10.3389/fpls.2021.792862. View

Wang F, Wang Q, Kwon S, Kwak S, Su W . Enhanced drought tolerance of transgenic rice plants expressing a pea manganese superoxide dismutase. J Plant Physiol. 2005; 162(4):465-72. DOI: 10.1016/j.jplph.2004.09.009. View

Su P, Yan J, Li W, Wang L, Zhao J, Ma X . A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress. BMC Plant Biol. 2020; 20(1):392. PMC: 7449071. DOI: 10.1186/s12870-020-02602-1. View

Tripathi P, Rabara R, Rushton P . A systems biology perspective on the role of WRKY transcription factors in drought responses in plants. Planta. 2013; 239(2):255-66. DOI: 10.1007/s00425-013-1985-y. View

Nanjo Y, Skultety L, Ashraf Y, Komatsu S . Comparative proteomic analysis of early-stage soybean seedlings responses to flooding by using gel and gel-free techniques. J Proteome Res. 2010; 9(8):3989-4002. DOI: 10.1021/pr100179f. View

Klinghammer M, Tenhaken R . Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls. J Exp Bot. 2007; 58(13):3609-21. DOI: 10.1093/jxb/erm209. View

Cooper R . Re-discovering ancient wheat varieties as functional foods. J Tradit Complement Med. 2015; 5(3):138-43. PMC: 4488568. DOI: 10.1016/j.jtcme.2015.02.004. View

Wang R, Ma J, Zhang Q, Wu C, Zhao H, Wu Y . Genome-wide identification and expression profiling of glutathione transferase gene family under multiple stresses and hormone treatments in wheat (Triticum aestivum L.). BMC Genomics. 2019; 20(1):986. PMC: 6916456. DOI: 10.1186/s12864-019-6374-x. View

10.

Cai K, Gao H, Wu X, Zhang S, Han Z, Chen X . The Ability to Regulate Transmembrane Potassium Transport in Root Is Critical for Drought Tolerance in Barley. Int J Mol Sci. 2019; 20(17). PMC: 6747136. DOI: 10.3390/ijms20174111. View

11.

Shilov I, Seymour S, Patel A, Loboda A, Tang W, Keating S . The Paragon Algorithm, a next generation search engine that uses sequence temperature values and feature probabilities to identify peptides from tandem mass spectra. Mol Cell Proteomics. 2007; 6(9):1638-55. DOI: 10.1074/mcp.T600050-MCP200. View

12.

Yan J, He C, Wang J, Mao Z, Holaday S, Allen R . Overexpression of the Arabidopsis 14-3-3 protein GF14 lambda in cotton leads to a "stay-green" phenotype and improves stress tolerance under moderate drought conditions. Plant Cell Physiol. 2004; 45(8):1007-14. DOI: 10.1093/pcp/pch115. View

13.

Shinozaki K, Yamaguchi-Shinozaki K . Gene networks involved in drought stress response and tolerance. J Exp Bot. 2006; 58(2):221-7. DOI: 10.1093/jxb/erl164. View

14.

Laus M, De Santis M, Flagella Z, Soccio M . Changes in Antioxidant Defence System in Durum Wheat under Hyperosmotic Stress: A Concise Overview. Plants (Basel). 2022; 11(1). PMC: 8747421. DOI: 10.3390/plants11010098. View

15.

Luo X, Bai X, Sun X, Zhu D, Liu B, Ji W . Expression of wild soybean WRKY20 in Arabidopsis enhances drought tolerance and regulates ABA signalling. J Exp Bot. 2013; 64(8):2155-69. DOI: 10.1093/jxb/ert073. View

16.

Hassan N, El-Bastawisy Z, El-Sayed A, Ebeed H, Nemat Alla M . Roles of dehydrin genes in wheat tolerance to drought stress. J Adv Res. 2015; 6(2):179-88. PMC: 4348445. DOI: 10.1016/j.jare.2013.11.004. View

17.

Ben Saad R, Harbaoui M, Ben Romdhane W, Zouari N, Giang K, Ben Hsouna A . Overexpression of Triticum durum TdAnn12 gene confers stress tolerance through scavenging reactive oxygen species in transgenic tobacco. Funct Plant Biol. 2019; 46(10):885-895. DOI: 10.1071/FP18316. View

18.

Voll L, Hajirezaei M, Czogalla-Peter C, Lein W, Stitt M, Sonnewald U . Antisense inhibition of enolase strongly limits the metabolism of aromatic amino acids, but has only minor effects on respiration in leaves of transgenic tobacco plants. New Phytol. 2009; 184(3):607-618. DOI: 10.1111/j.1469-8137.2009.02998.x. View

19.

Fu H, Subramanian R, Masters S . 14-3-3 proteins: structure, function, and regulation. Annu Rev Pharmacol Toxicol. 2000; 40:617-47. DOI: 10.1146/annurev.pharmtox.40.1.617. View

20.

Joshi R, Karan R, Singla-Pareek S, Pareek A . Ectopic expression of Pokkali phosphoglycerate kinase-2 (OsPGK2-P) improves yield in tobacco plants under salinity stress. Plant Cell Rep. 2015; 35(1):27-41. DOI: 10.1007/s00299-015-1864-z. View