Enhancement of Human Epidermal Cell Defense Against UVB Damage by Fermentation of Sims Peel with

Overview

Journal Nutrients

Date 2023 Feb 11

PMID 36771204

Authors

Jiaxuan Fang

Qianru Sun

Ziwen Wang

Zixin Song

Jiman Geng

Changtao Wang

Meng Li

Dongdong Wang

Affiliations

Soon will be listed here.

Abstract

The processing of Sims results in large amounts of wasted peel resources and environmental pollution. In order to improve the utilisation of natural plant resources and economic benefits, this study uses to ferment Sims peel to obtain Sims peel fermentation broth (PF). The content of active substances in unfermented Sims peel water extract (PW) and PF is then determined, as well as their in vitro antioxidant capacity. The protective effects of PF and PW on UVB-induced skin inflammation and skin barrier damage in human immortalised epidermal keratinocytes (HaCaT) cells (including cell viability, ROS, HO-1, NQO1, IL-1β, IL-8, TNF-α, KLK-7, FLG, AQP3 and Caspase 14 levels) are investigated. Studies have shown that PF enhances the content of active substances more effectively compared to PW, showing a superior ability to scavenge free radical scavenging and antioxidants. PW and PF can effectively scavenge excess intracellular ROS, reduce the cellular secretion of pro-inflammatory factors, regulate the content of skin barrier-related proteins and possibly respond to UVB-induced cell damage by inhibiting the activation of the PI3K/AKT/mTOR signalling pathway. Studies have shown that both PW and PF are safe and non-irritating, with PF exploiting the efficacy of Sims peel more significantly, providing a superior process for the utilisation of Sims waste. At the same time, PF can be developed and used as a functional protective agent against ultraviolet damage to the skin, thereby increasing the value of the use of Sims waste.

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References

Kishibe M . Physiological and pathological roles of kallikrein-related peptidases in the epidermis. J Dermatol Sci. 2019; 95(2):50-55. DOI: 10.1016/j.jdermsci.2019.06.007. View

Hu Q, Zheng J, Xu X, Gu C, Li W . Uranium induces kidney cells apoptosis via reactive oxygen species generation, endoplasmic reticulum stress and inhibition of PI3K/AKT/mTOR signaling in culture. Environ Toxicol. 2022; 37(4):899-909. DOI: 10.1002/tox.23453. View

Burger C, Shirsath N, Lang V, Diehl S, Kaufmann R, Weigert A . Blocking mTOR Signalling with Rapamycin Ameliorates Imiquimod-induced Psoriasis in Mice. Acta Derm Venereol. 2017; 97(9):1087-1094. DOI: 10.2340/00015555-2724. View

Oh Y, Lim H, Kim K, Lim C . Ginsenoside Re improves skin barrier function in HaCaT keratinocytes under normal growth conditions. Biosci Biotechnol Biochem. 2016; 80(11):2165-2167. DOI: 10.1080/09168451.2016.1206808. View

Piao M, Choi J, Lee D, Yun S, Lee J, Lee S . Differentiation-dependent expression of NADP(H):quinone oxidoreductase-1 via NF-E2 related factor-2 activation in human epidermal keratinocytes. J Dermatol Sci. 2011; 62(3):147-53. DOI: 10.1016/j.jdermsci.2011.02.003. View

Bridgeman B, Wang P, Ye B, Pelling J, Volpert O, Tong X . Inhibition of mTOR by apigenin in UVB-irradiated keratinocytes: A new implication of skin cancer prevention. Cell Signal. 2016; 28(5):460-468. PMC: 4788564. DOI: 10.1016/j.cellsig.2016.02.008. View

Endoh I, Di Girolamo N, Hampartzoumian T, Cameron B, Geczy C, Tedla N . Ultraviolet B irradiation selectively increases the production of interleukin-8 in human cord blood-derived mast cells. Clin Exp Immunol. 2007; 148(1):161-7. PMC: 1868850. DOI: 10.1111/j.1365-2249.2007.03332.x. View

Dos Reis L, Facco E, Salvador M, Flores S, Rios A . Antioxidant potential and physicochemical characterization of yellow, purple and orange passion fruit. J Food Sci Technol. 2018; 55(7):2679-2691. PMC: 6033812. DOI: 10.1007/s13197-018-3190-2. View

Chittock J, Cooke A, Lavender T, Brown K, Wigley A, Victor S . Development of stratum corneum chymotrypsin-like protease activity and natural moisturizing factors from birth to 4 weeks of age compared with adults. Br J Dermatol. 2016; 175(4):713-20. DOI: 10.1111/bjd.14568. View

10.

Lee S, Takagi Y, Nishizaka T, Baek J, Kim H, Lee S . Subclinical cutaneous inflammation remained after permeability barrier disruption enhances UV sensitivity by altering ER stress responses and topical pseudoceramide prevents them. Arch Dermatol Res. 2017; 309(7):541-550. DOI: 10.1007/s00403-017-1753-0. View

11.

Koundouros N, Poulogiannis G . Phosphoinositide 3-Kinase/Akt Signaling and Redox Metabolism in Cancer. Front Oncol. 2018; 8:160. PMC: 5968394. DOI: 10.3389/fonc.2018.00160. View

12.

McGovern J, Meinert C, de Veer S, Hollier B, Parker T, Upton Z . Attenuated kallikrein-related peptidase activity disrupts desquamation and leads to stratum corneum thickening in human skin equivalent models. Br J Dermatol. 2016; 176(1):145-158. DOI: 10.1111/bjd.14879. View

13.

Hoste E, Kemperman P, Devos M, Denecker G, Kezic S, Yau N . Caspase-14 is required for filaggrin degradation to natural moisturizing factors in the skin. J Invest Dermatol. 2011; 131(11):2233-41. DOI: 10.1038/jid.2011.153. View

14.

Hsu W, Lu J, Noda M, Wu C, Liu J, Sakakibara M . Derinat Protects Skin against Ultraviolet-B (UVB)-Induced Cellular Damage. Molecules. 2015; 20(11):20297-311. PMC: 6331914. DOI: 10.3390/molecules201119693. View

15.

Gao S, Guo K, Chen Y, Zhao J, Jing R, Wang L . Keratinocyte Growth Factor 2 Ameliorates UVB-Induced Skin Damage Activating the AhR/Nrf2 Signaling Pathway. Front Pharmacol. 2021; 12:655281. PMC: 8215442. DOI: 10.3389/fphar.2021.655281. View

16.

Wagener F, Carels C, Lundvig D . Targeting the redox balance in inflammatory skin conditions. Int J Mol Sci. 2013; 14(5):9126-67. PMC: 3676777. DOI: 10.3390/ijms14059126. View

17.

Numata I, Okuyama R, Memezawa A, Ito Y, Takeda K, Furuyama K . Functional expression of heme oxygenase-1 in human differentiated epidermis and its regulation by cytokines. J Invest Dermatol. 2009; 129(11):2594-603. DOI: 10.1038/jid.2009.119. View

18.

Chen H, Wang C, Tang B, Yu J, Lu Y, Zhang J . Peel Polysaccharides Ameliorate Imiquimod-Induced Psoriasis-Like Dermatitis in Mice Suppression of NF-κB and STAT3 Pathways. Front Pharmacol. 2022; 12:806844. PMC: 8831316. DOI: 10.3389/fphar.2021.806844. View

19.

Lee Y, Je Y, Lee S, Li Z, Choi D, Kwon Y . Changes in transepidermal water loss and skin hydration according to expression of aquaporin-3 in psoriasis. Ann Dermatol. 2012; 24(2):168-74. PMC: 3346907. DOI: 10.5021/ad.2012.24.2.168. View

20.

Takagi Y, Nakagawa H, Kondo H, Takema Y, Imokawa G . Decreased levels of covalently bound ceramide are associated with ultraviolet B-induced perturbation of the skin barrier. J Invest Dermatol. 2004; 123(6):1102-9. DOI: 10.1111/j.0022-202X.2004.23491.x. View