Ulvophyte Green Algae : Metabolites Profile and Antioxidant, Anticancer, Anti-Obesity, and In Vitro Cytotoxicity Properties

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Feb 11

PMID 36771032

Authors

Fahrul Nurkolis

Nurpudji Astuti Taslim

Faqrizal Ria Qhabibi

Sojin Kang

Myunghan Moon

Jinwon Choi

Min Choi

Moon Nyeo Park

Nelly Mayulu

Bonglee Kim

Affiliations

Soon will be listed here.

Abstract

Marine algae have excellent bioresource properties with potential nutritional and bioactive therapeutic benefits, but studies regarding are limited. This study aims to explore the metabolites profile and the antioxidant, anticancer, anti-obesity, and in vitro cytotoxicity properties of fractionated ethanolic extract of using two maceration and soxhlet extraction methods. Dried simplicia of was mashed and extracted in ethanol solvent, concentrated and evaporated, then sequentially partitioned with equal volumes of ethyl acetate and -Hexane. Six samples were used in this study, consisting of ME (Maceration-Ethanol), MEA (Maceration-Ethyl Acetate), MH (Maceration--Hexane), SE (Soxhletation-Ethanol), SEA (Soxhletation-Ethyl Acetate), and SH (Soxhletation--Hexane). Non-targeted metabolomic profiling was determined using LC-HRMS, while antioxidant, anti-obesity, and anticancer cytotoxicity were determined using DPPH and ABTS, lipase inhibition, and MTT assay, respectively. This study demonstrates that has several functional metabolites, antioxidant capacity (EC MH is very close to EC of Trolox), as well as anti-obesity properties (EC MH < EC orlistat, an inhibitor of lipid hydrolyzing enzymes), which are useful as precursors for new therapeutic approaches in improving obesity-related diseases. More interestingly, ME, MH, and SE are novel bioresource agents for anticancer drugs, especially for hepatoma, breast, colorectal, and leukemia cancers. Finally, can be a nutraceutical with great therapeutic benefits.

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