Postbiotics Derived from ET-22 Inhibit the Formation of Biofilms and Bioactive Substances: An Analysis

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Feb 11

PMID 36770903

Authors

Zhi Zhao

Jianmin Wu

Zhe Sun

Jinbo Fan

Fudong Liu

Wen Zhao

Wei-Hsien Liu

Ming Zhang

Wei-Lian Hung

Affiliations

Soon will be listed here.

Abstract

Globally, dental caries is one of the most common non-communicable diseases for patients of all ages; () is its principal pathogen. () shows excellent anti-pathogens and immune-regulation functions in the host. The aim of this study is to evaluate the effects of ET-22 on the formation of biofilms. The living bacteria, heat-killed bacteria, and secretions of ET-22 were prepared using the same number of bacteria. In vitro, they were added into artificial-saliva medium, and used to coculture with the . Results showed that the living bacteria and secretions of ET-22 inhibited biofilm-growth, the synthesis of water-soluble polysaccharide and water-insoluble polysaccharide, and virulence-gene-expression levels related to the formation of biofilms. Surprisingly, the heat-killed ET-22, which is a postbiotic, also showed a similar regulation function. Non-targeted metabonomics technology was used to identify multiple potential active-substances in the postbiotics of ET-22 that inhibit the formation of biofilms, including phenyllactic acid, zidovudine monophosphate, and citrulline. In conclusion, live bacteria and its postbiotics of ET-22 all have inhibitory effects on the formation of biofilm. The postbiotics of ET-22 may be a promising biological anticariogenic-agent.

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References

Liu Y, Zhao Q . Influence of surface energy of modified surfaces on bacterial adhesion. Biophys Chem. 2005; 117(1):39-45. DOI: 10.1016/j.bpc.2005.04.015. View

Al-Ansari M, Al-Dahmash N, Ranjitsingh A . Synthesis of silver nanoparticles using gum Arabic: Evaluation of its inhibitory action on Streptococcus mutans causing dental caries and endocarditis. J Infect Public Health. 2021; 14(3):324-330. PMC: 7895472. DOI: 10.1016/j.jiph.2020.12.016. View

Bidossi A, De Grandi R, Toscano M, Bottagisio M, De Vecchi E, Gelardi M . Probiotics Streptococcus salivarius 24SMB and Streptococcus oralis 89a interfere with biofilm formation of pathogens of the upper respiratory tract. BMC Infect Dis. 2018; 18(1):653. PMC: 6292094. DOI: 10.1186/s12879-018-3576-9. View

Xiong Z, Zhu X, Geng J, Xu Y, Wu R, Li C . Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine. Immunity. 2022; 55(4):686-700.e7. DOI: 10.1016/j.immuni.2022.03.001. View

Gong T, He X, Chen J, Tang B, Zheng T, Jing M . Transcriptional Profiling Reveals the Importance of RcrR in the Regulation of Multiple Sugar Transportation and Biofilm Formation in Streptococcus mutans. mSystems. 2021; 6(4):e0078821. PMC: 8407328. DOI: 10.1128/mSystems.00788-21. View

Heydorn A, Nielsen A, Hentzer M, Sternberg C, Givskov M, Ersboll B . Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology (Reading). 2000; 146 ( Pt 10):2395-2407. DOI: 10.1099/00221287-146-10-2395. View

Sun Y, Jiang W, Zhang M, Zhang L, Shen Y, Huang S . The Inhibitory Effects of Ficin on Biofilm Formation. Biomed Res Int. 2021; 2021:6692328. PMC: 8009705. DOI: 10.1155/2021/6692328. View

Shakya S, Danshiitsoodol N, Noda M, Inoue Y, Sugiyama M . 3-Phenyllactic acid generated in medicinal plant extracts fermented with plant-derived lactic acid bacteria inhibits the biofilm synthesis of . Front Microbiol. 2022; 13:991144. PMC: 9539679. DOI: 10.3389/fmicb.2022.991144. View

Pertusati F, Pileggi E, Richards J, Wootton M, Van Leemputte T, Persoons L . Drug repurposing: phosphate prodrugs of anticancer and antiviral FDA-approved nucleosides as novel antimicrobials. J Antimicrob Chemother. 2020; 75(10):2864-2878. DOI: 10.1093/jac/dkaa268. View

10.

Chen H, Zhang B, Weir M, Homayounfar N, Fay G, Martinho F . S. mutans gene-modification and antibacterial resin composite as dual strategy to suppress biofilm acid production and inhibit caries. J Dent. 2020; 93:103278. DOI: 10.1016/j.jdent.2020.103278. View

11.

Cao L, Zhang Z, Xu S, Ma M, Wei X . Farnesol inhibits development of caries by augmenting oxygen sensitivity and suppressing virulence-associated gene expression inStreptococcus mutans. J Biomed Res. 2017; 31(4):333-343. PMC: 5548994. DOI: 10.7555/JBR.31.20150151. View

12.

Zhang J, Wang D, Sun J, Sun Z, Liu F, Du L . Synergistic Antibiofilm Effects of Ultrasound and Phenyllactic Acid against and . Foods. 2021; 10(9). PMC: 8466041. DOI: 10.3390/foods10092171. View

13.

Bowen W, Koo H . Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res. 2011; 45(1):69-86. PMC: 3068567. DOI: 10.1159/000324598. View

14.

Teughels W, Van Assche N, Sliepen I, Quirynen M . Effect of material characteristics and/or surface topography on biofilm development. Clin Oral Implants Res. 2006; 17 Suppl 2:68-81. DOI: 10.1111/j.1600-0501.2006.01353.x. View

15.

Depommier C, Everard A, Druart C, Plovier H, Van Hul M, Vieira-Silva S . Supplementation with Akkermansia muciniphila in overweight and obese human volunteers: a proof-of-concept exploratory study. Nat Med. 2019; 25(7):1096-1103. PMC: 6699990. DOI: 10.1038/s41591-019-0495-2. View

16.

Maehata H, Arai S, Iwabuchi N, Abe F . Immuno-modulation by heat-killed MCC1849 and its application to food products. Int J Immunopathol Pharmacol. 2021; 35:20587384211008291. PMC: 8020404. DOI: 10.1177/20587384211008291. View

17.

Hannig C, Hannig M . The oral cavity--a key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin Oral Investig. 2009; 13(2):123-39. DOI: 10.1007/s00784-008-0243-3. View

18.

Wasfi R, Abd El-Rahman O, Zafer M, Ashour H . Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans. J Cell Mol Med. 2018; 22(3):1972-1983. PMC: 5824418. DOI: 10.1111/jcmm.13496. View

19.

Jiang Q, Stamatova I, Kainulainen V, Korpela R, Meurman J . Interactions between Lactobacillus rhamnosus GG and oral micro-organisms in an in vitro biofilm model. BMC Microbiol. 2016; 16(1):149. PMC: 4942979. DOI: 10.1186/s12866-016-0759-7. View

20.

Lu J, Cheng L, Huang Y, Jiang Y, Chu C, Peng X . Resumptive Persisters Induced From Dimethylaminododecyl Methacrylate Elevated the Cariogenic Virulence by Up-Regulating the Quorum-Sensing and VicRK Pathway Genes. Front Microbiol. 2020; 10:3102. PMC: 6985435. DOI: 10.3389/fmicb.2019.03102. View