[Effect of on the Biological Functions of Acute B Lymphoblastic Leukemia Cell Line REH]

Objective: To discover the relationship between matrix remodeling associated 7 () and acute B lymphoblastic leukemia (B-ALL), and explore the effect of on the biological functions of B-ALL cell line REH.

Methods: The expression of in blood diseases was searched and analyzed through BloodSpot database. Real-time qPCR was used to detect the expression level of in B-ALL cell line 697 and REH cells. Lentivirus-mediated shRNA interference technology was utilized to knock down the expression of in REH cells. The effects of on the biological functions of REH cells were studied by in vitro experiments. Cell proliferation was detected by CCK-8 assay, cell cycle was detected by PI staining, cell apoptosis was detected by Annexin V and 7-AAD staining, and the expression of apoptosis pathway related proteins was detected by Western blot.

Results: Database analysis showed that was highly expressed in B-ALL patients, and real-time qPCR results showed that was also highly expressed in cell lines 697 and REH cells. Knockdown of in REH cells inhibited the cell proliferation and increased the percentage of G/G phase cells. After treatment with cytarabine, the apoptotic ratio was increased in -impaired REH cells, and the activation of caspase-3 and caspase-9 were also increased.

Conclusion: Knockdown of can reduce the malignancy of REH cells by inhibiting the cell proliferation and increasing the sensitivity of REH cells to cytarabine. These results indicate may be as a novel target for the treatment of B-ALL, and the potential usefulness of in B-ALL deserves further investigation.