Subtype Distribution of Sp. Isolated from Humans in Iran: a Systematic Review and Meta-analysis

Overview

Journal Gastroenterol Hepatol Bed Bench

Specialty Gastroenterology

Date 2023 Feb 10

PMID 36762220

Authors

Bahman Maleki

Meysam Olfatifar

Samira Dodangeh

Nayebali Ahmadi

Mohammad Gorgipour

Ehsan Javanmard

Affiliations

Soon will be listed here.

Abstract

Aim: This systematic review and meta-analysis evaluated the subtyped sp. isolated from humans in Iran.

Background: sp. is an anaerobic intestinal protozoan that infects humans as well as domestic and wild animals, i.e. mammals, amphibians, reptiles, and arthropods.

Methods: A comprehensive search for papers published before April 2022 was undertaken utilizing English and Persian databases. The following MeSH keywords were used in the electronic search: ( sp.) AND (molecular OR subtype) AND (prevalence OR epidemiology) AND Iran. The quality of the included studies was evaluated. Thereafter, a random-effects meta-analysis was conducted to estimate the pooled prevalence and odds ratios regarding the included studies.

Results: A total of 32 studies comprised of five case-control studies and 27 cross-sectional studies met the eligibility criteria. The overall pooled prevalence of subtyped sp. in Iran was estimated to be 10% (95% confidence interval: 6 to 15%). Eight subtypes of sp. (ST1- ST7 and ST9) were identified in our study, of which ST3 was the most common subtype (0.04); 0.02-0.07). The difference in subtypes between two case and control groups in reported studies was not significant, but the odds ratio of infection by ST3 (0.98; 95% CI, 0.30 to 3.20) was higher in cases.

Conclusion: The current systematic review showed that with the exception of ST8 and ST12, all human sp. subtypes reported in the world are found in different parts of Iran.

Citing Articles

Molecular identification and subtypes analysis of Blastocystis in South Khorasan province, Eastern Iran.

Behravan M, Haghighi A, Javanmard E, Mohebi M, Nemati S Mol Biol Rep. 2024; 52(1):80.

PMID: 39718658 DOI: 10.1007/s11033-024-10135-1.

Prevalence and subtyping of Blastocystis sp. in ruminants in Southwestern, Iran.

Heydarian M, Manouchehri Naeini K, Kheiri S, Abdizadeh R Sci Rep. 2024; 14(1):20254.

PMID: 39215121 PMC: 11364837. DOI: 10.1038/s41598-024-70907-4.

References

Stensvold C, Alfellani M, Clark C . Levels of genetic diversity vary dramatically between Blastocystis subtypes. Infect Genet Evol. 2011; 12(2):263-73. DOI: 10.1016/j.meegid.2011.11.002. View

Piranshahi A, Tavalla M, Khademvatan S . Genomic analysis of isolates in patients with HIV-positive using locus SSU-rDNA. J Parasit Dis. 2018; 42(1):28-33. PMC: 5825359. DOI: 10.1007/s12639-017-0957-8. View

Tan K, Mirza H, Teo J, Wu B, Macary P . Current Views on the Clinical Relevance of Blastocystis spp. Curr Infect Dis Rep. 2011; 12(1):28-35. DOI: 10.1007/s11908-009-0073-8. View

Alinaghizade A, Mirjalali H, Mohebali M, Stensvold C, Rezaeian M . Inter- and intra-subtype variation of Blastocystis subtypes isolated from diarrheic and non-diarrheic patients in Iran. Infect Genet Evol. 2017; 50:77-82. DOI: 10.1016/j.meegid.2017.02.016. View

Khoshnood S, Rafiei A, Saki J, Alizadeh K . Prevalence and Genotype Characterization of Blastocystis hominis Among the Baghmalek People in Southwestern Iran in 2013 - 2014. Jundishapur J Microbiol. 2015; 8(10):e23930. PMC: 4644266. DOI: 10.5812/jjm.23930. View

Scicluna S, Tawari B, Clark C . DNA barcoding of blastocystis. Protist. 2006; 157(1):77-85. DOI: 10.1016/j.protis.2005.12.001. View

Maleki B, Sadraei J, Dalimi Asl A, Pirestani M . High occurrence of sp. subtype 3 in individuals referred to medical laboratories in Kermanshah, Iran. Gastroenterol Hepatol Bed Bench. 2022; 15(2):164-171. PMC: 9275734. View

Leder K, Hellard M, Sinclair M, Fairley C, Wolfe R . No correlation between clinical symptoms and Blastocystis hominis in immunocompetent individuals. J Gastroenterol Hepatol. 2005; 20(9):1390-4. DOI: 10.1111/j.1440-1746.2005.03868.x. View

Ramirez J, Sanchez L, Bautista D, Corredor A, Florez A, Stensvold C . Blastocystis subtypes detected in humans and animals from Colombia. Infect Genet Evol. 2013; 22:223-8. DOI: 10.1016/j.meegid.2013.07.020. View

10.

Sohail M, Fischer P . Blastocystis hominis and travelers. Travel Med Infect Dis. 2007; 3(1):33-8. DOI: 10.1016/j.tmaid.2004.06.001. View

11.

Alfellani M, Taner-Mulla D, Jacob A, Imeede C, Yoshikawa H, Stensvold C . Genetic diversity of blastocystis in livestock and zoo animals. Protist. 2013; 164(4):497-509. DOI: 10.1016/j.protis.2013.05.003. View

12.

Khademvatan S, Masjedizadeh R, Yousefi-Razin E, Mahbodfar H, Rahim F, Yousefi E . PCR-based molecular characterization of Blastocystis hominis subtypes in southwest of Iran. J Infect Public Health. 2017; 11(1):43-47. DOI: 10.1016/j.jiph.2017.03.009. View

13.

Mattiucci S, Crisafi B, Gabrielli S, Paoletti M, Cancrini G . Molecular epidemiology and genetic diversity of Blastocystis infection in humans in Italy. Epidemiol Infect. 2015; 144(3):635-46. DOI: 10.1017/S0950268815001697. View

14.

Sharifi Y, Abbasi F, Shahabi S, Zaraei A, Mikaeili F, Sarkari B . Comparative genotyping of Blastocystis infecting cattle and human in the south of Iran. Comp Immunol Microbiol Infect Dis. 2020; 72:101529. DOI: 10.1016/j.cimid.2020.101529. View

15.

Ramirez J, Sanchez A, Hernandez C, Florez C, Bernal M, Giraldo J . Geographic distribution of human Blastocystis subtypes in South America. Infect Genet Evol. 2016; 41:32-35. DOI: 10.1016/j.meegid.2016.03.017. View

16.

Bahrami F, Babaei E, Badirzadeh A, Riabi T, Abdoli A . Blastocystis, urticaria, and skin disorders: review of the current evidences. Eur J Clin Microbiol Infect Dis. 2019; 39(6):1027-1042. DOI: 10.1007/s10096-019-03793-8. View

17.

Maloney J, Lombard J, Urie N, Shivley C, Santin M . Zoonotic and genetically diverse subtypes of Blastocystis in US pre-weaned dairy heifer calves. Parasitol Res. 2018; 118(2):575-582. DOI: 10.1007/s00436-018-6149-3. View

18.

Taghipour A, Javanmard E, Mirjalali H, Haghighi A, Tabarsi P, Sohrabi M . Blastocystis subtype 1 (allele 4); Predominant subtype among tuberculosis patients in Iran. Comp Immunol Microbiol Infect Dis. 2019; 65:201-206. DOI: 10.1016/j.cimid.2019.06.005. View

19.

Mirjalali H, Abbasi M, Naderi N, Hasani Z, Mirsamadi E, Stensvold C . Distribution and phylogenetic analysis of Blastocystis sp. subtypes isolated from IBD patients and healthy individuals in Iran. Eur J Clin Microbiol Infect Dis. 2017; 36(12):2335-2342. DOI: 10.1007/s10096-017-3065-x. View

20.

Asghari A, Zare M, Hatam G, Shahabi S, Gholizadeh F, Motazedian M . Molecular identification and subtypes distribution of Blastocystis sp. isolated from children and adolescent with cancer in Iran: evaluation of possible risk factors and clinical features. Acta Parasitol. 2020; 65(2):462-473. DOI: 10.2478/s11686-020-00186-2. View